Genome analysis of Bifidobacterium adolescentis and investigation of its effects on inflammation and intestinal barrier function
Numerous studies have confirmed that gut microbiota is a key driver in the occurrence and progression of inflammatory bowel disease (IBD). Based on the bacterial collection constructed in our previous studies, we founded that Bifidobacterium adolescentis AF91-08b2A has the potential beneficial funct...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1496280/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832591680435388416 |
|---|---|
| author | Bo Li Bo Li Haoyu Wang Haoyu Wang Haoyu Wang Mengmeng Wang Mengmeng Wang Hewei Liang Hewei Liang Hewei Liang Tongyuan Hu Tongyuan Hu Tongyuan Hu Jinlong Yang Jinlong Yang Jinlong Yang Shangyong Li Xinbi You Xinbi You Xinbi You Binbin Xia Binbin Xia Binbin Xia Yue Yuan Yue Yuan Yuanqiang Zou Yuanqiang Zou Yinglei Miao Yinglei Miao Yang Sun Yang Sun Yang Sun |
| author_facet | Bo Li Bo Li Haoyu Wang Haoyu Wang Haoyu Wang Mengmeng Wang Mengmeng Wang Hewei Liang Hewei Liang Hewei Liang Tongyuan Hu Tongyuan Hu Tongyuan Hu Jinlong Yang Jinlong Yang Jinlong Yang Shangyong Li Xinbi You Xinbi You Xinbi You Binbin Xia Binbin Xia Binbin Xia Yue Yuan Yue Yuan Yuanqiang Zou Yuanqiang Zou Yinglei Miao Yinglei Miao Yang Sun Yang Sun Yang Sun |
| author_sort | Bo Li |
| collection | DOAJ |
| description | Numerous studies have confirmed that gut microbiota is a key driver in the occurrence and progression of inflammatory bowel disease (IBD). Based on the bacterial collection constructed in our previous studies, we founded that Bifidobacterium adolescentis AF91-08b2A has the potential beneficial function. We designed cohort studies, genomic studies and animal experiments to further explore the probiotic function of Bifidobacterium adolescentis AF91-08b2A and its therapeutic effect on IBD. The depletion of B. adolescentis in individuals with IBD suggested its significance for intestinal health. Genomic analysis highlighted the probiotic attributes of B. adolescentis AF91-08b2A, including resistance to antibiotics and stress, and metabolic pathways related to energy and carbohydrate metabolism, which are likely to enhance its therapeutic efficacy. In DSS-induced mice colitis model, the strain significantly enhanced the disease activity index (DAI), curbed weight loss, and attenuated colonic damage. It effectively modulated the immune response by reducing the levels of pro-inflammatory cytokines such as IL-6, IL-1β, IL-17A, IFN-γ, and TNF-α, while promoting the secretion of anti-inflammatory cytokines like IL-4, IL-10, and TGF-β1. The restoration of tight junction proteins ZO-1, occludin, and claudin-2 by B. adolescentis AF91-08b2A demonstrated its capacity to safeguard the intestinal epithelial barrier. Collectively, our findings indicate B. adolescentis AF91-08b2A as a valuable therapeutic option for UC, with its multifaceted approach to reducing inflammation and fortifying the intestinal barrier. |
| format | Article |
| id | doaj-art-1ebc22b6d878443d82e3839faaf65f0e |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-1ebc22b6d878443d82e3839faaf65f0e2025-01-22T07:10:59ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011510.3389/fmicb.2024.14962801496280Genome analysis of Bifidobacterium adolescentis and investigation of its effects on inflammation and intestinal barrier functionBo Li0Bo Li1Haoyu Wang2Haoyu Wang3Haoyu Wang4Mengmeng Wang5Mengmeng Wang6Hewei Liang7Hewei Liang8Hewei Liang9Tongyuan Hu10Tongyuan Hu11Tongyuan Hu12Jinlong Yang13Jinlong Yang14Jinlong Yang15Shangyong Li16Xinbi You17Xinbi You18Xinbi You19Binbin Xia20Binbin Xia21Binbin Xia22Yue Yuan23Yue Yuan24Yuanqiang Zou25Yuanqiang Zou26Yinglei Miao27Yinglei Miao28Yang Sun29Yang Sun30Yang Sun31Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaYunnan Province Clinical Research Center for Digestive Diseases, Kunming, ChinaBGI Research, Shenzhen, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaSchool of Basic Medicine, Qingdao Medical College, Qingdao University, Qingdao, ChinaBGI Research, Shenzhen, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaBGI Research, Shenzhen, ChinaBGI Research, Wuhan, ChinaShenzhen Engineering Laboratory of Detection and Intervention of Human Intestinal Microbiome, BGI Research, Shenzhen, ChinaBGI Research, Shenzhen, ChinaBGI Research, Wuhan, ChinaShenzhen Engineering Laboratory of Detection and Intervention of Human Intestinal Microbiome, BGI Research, Shenzhen, ChinaBGI Research, Shenzhen, ChinaBGI Research, Kunming, ChinaCollege of Forensic Science, Xi’an Jiaotong University, Xi’an, ChinaSchool of Basic Medicine, Qingdao Medical College, Qingdao University, Qingdao, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaYunnan Province Clinical Research Center for Digestive Diseases, Kunming, China0Yunnan Geriatric Medical Center, Kunming, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaYunnan Province Clinical Research Center for Digestive Diseases, Kunming, China0Yunnan Geriatric Medical Center, Kunming, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaYunnan Province Clinical Research Center for Digestive Diseases, Kunming, ChinaBGI Research, Shenzhen, ChinaShenzhen Engineering Laboratory of Detection and Intervention of Human Intestinal Microbiome, BGI Research, Shenzhen, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaYunnan Province Clinical Research Center for Digestive Diseases, Kunming, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaYunnan Province Clinical Research Center for Digestive Diseases, Kunming, China0Yunnan Geriatric Medical Center, Kunming, ChinaNumerous studies have confirmed that gut microbiota is a key driver in the occurrence and progression of inflammatory bowel disease (IBD). Based on the bacterial collection constructed in our previous studies, we founded that Bifidobacterium adolescentis AF91-08b2A has the potential beneficial function. We designed cohort studies, genomic studies and animal experiments to further explore the probiotic function of Bifidobacterium adolescentis AF91-08b2A and its therapeutic effect on IBD. The depletion of B. adolescentis in individuals with IBD suggested its significance for intestinal health. Genomic analysis highlighted the probiotic attributes of B. adolescentis AF91-08b2A, including resistance to antibiotics and stress, and metabolic pathways related to energy and carbohydrate metabolism, which are likely to enhance its therapeutic efficacy. In DSS-induced mice colitis model, the strain significantly enhanced the disease activity index (DAI), curbed weight loss, and attenuated colonic damage. It effectively modulated the immune response by reducing the levels of pro-inflammatory cytokines such as IL-6, IL-1β, IL-17A, IFN-γ, and TNF-α, while promoting the secretion of anti-inflammatory cytokines like IL-4, IL-10, and TGF-β1. The restoration of tight junction proteins ZO-1, occludin, and claudin-2 by B. adolescentis AF91-08b2A demonstrated its capacity to safeguard the intestinal epithelial barrier. Collectively, our findings indicate B. adolescentis AF91-08b2A as a valuable therapeutic option for UC, with its multifaceted approach to reducing inflammation and fortifying the intestinal barrier.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1496280/fullBifidobacterium adolescentisinflammatory bowel diseaseprobiotic functionintestinal barrier functiongenomic analysis |
| spellingShingle | Bo Li Bo Li Haoyu Wang Haoyu Wang Haoyu Wang Mengmeng Wang Mengmeng Wang Hewei Liang Hewei Liang Hewei Liang Tongyuan Hu Tongyuan Hu Tongyuan Hu Jinlong Yang Jinlong Yang Jinlong Yang Shangyong Li Xinbi You Xinbi You Xinbi You Binbin Xia Binbin Xia Binbin Xia Yue Yuan Yue Yuan Yuanqiang Zou Yuanqiang Zou Yinglei Miao Yinglei Miao Yang Sun Yang Sun Yang Sun Genome analysis of Bifidobacterium adolescentis and investigation of its effects on inflammation and intestinal barrier function Frontiers in Microbiology Bifidobacterium adolescentis inflammatory bowel disease probiotic function intestinal barrier function genomic analysis |
| title | Genome analysis of Bifidobacterium adolescentis and investigation of its effects on inflammation and intestinal barrier function |
| title_full | Genome analysis of Bifidobacterium adolescentis and investigation of its effects on inflammation and intestinal barrier function |
| title_fullStr | Genome analysis of Bifidobacterium adolescentis and investigation of its effects on inflammation and intestinal barrier function |
| title_full_unstemmed | Genome analysis of Bifidobacterium adolescentis and investigation of its effects on inflammation and intestinal barrier function |
| title_short | Genome analysis of Bifidobacterium adolescentis and investigation of its effects on inflammation and intestinal barrier function |
| title_sort | genome analysis of bifidobacterium adolescentis and investigation of its effects on inflammation and intestinal barrier function |
| topic | Bifidobacterium adolescentis inflammatory bowel disease probiotic function intestinal barrier function genomic analysis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1496280/full |
| work_keys_str_mv | AT boli genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT boli genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT haoyuwang genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT haoyuwang genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT haoyuwang genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT mengmengwang genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT mengmengwang genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT heweiliang genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT heweiliang genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT heweiliang genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT tongyuanhu genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT tongyuanhu genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT tongyuanhu genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT jinlongyang genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT jinlongyang genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT jinlongyang genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT shangyongli genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT xinbiyou genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT xinbiyou genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT xinbiyou genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT binbinxia genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT binbinxia genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT binbinxia genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT yueyuan genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT yueyuan genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT yuanqiangzou genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT yuanqiangzou genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT yingleimiao genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT yingleimiao genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT yangsun genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT yangsun genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction AT yangsun genomeanalysisofbifidobacteriumadolescentisandinvestigationofitseffectsoninflammationandintestinalbarrierfunction |