Glucomannan engineering highlights roles of galactosyl modification in fine-tuning cellulose-glucomannan interaction in Arabidopsis cell walls
Abstract Widely found in most plant lineages, β-mannans are structurally diverse polysaccharides that can bind to cellulose fibrils to form the complex polysaccharide architecture of the cell wall. How changes in polysaccharide structure influence its cell wall solubility or promote appropriate inte...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-56626-y |
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| author | Yoshihisa Yoshimi Li Yu Rosalie Cresswell Xinyu Guo Alberto Echevarría-Poza Jan J. Lyczakowski Ray Dupree Toshihisa Kotake Paul Dupree |
| author_facet | Yoshihisa Yoshimi Li Yu Rosalie Cresswell Xinyu Guo Alberto Echevarría-Poza Jan J. Lyczakowski Ray Dupree Toshihisa Kotake Paul Dupree |
| author_sort | Yoshihisa Yoshimi |
| collection | DOAJ |
| description | Abstract Widely found in most plant lineages, β-mannans are structurally diverse polysaccharides that can bind to cellulose fibrils to form the complex polysaccharide architecture of the cell wall. How changes in polysaccharide structure influence its cell wall solubility or promote appropriate interaction with cellulose fibrils is poorly understood. Glucomannan backbones acquire variable patterns of galactosyl substitutions, depending on plant developmental stage and species. Here, we show that fine-tuning of galactosyl modification on glucomannans is achieved by the differing acceptor recognition of mannan α-galactosyltransferases (MAGTs). Biochemical analysis and 13C solid-state nuclear magnetic resonance spectroscopy of Arabidopsis with cell wall glucomannan engineered by MAGTs reveal that the degree of galactosylation strongly affects the interaction with cellulose. The findings indicate that plants tailor galactosyl modification on glucomannans for constructing an appropriate cell wall architecture, paving the way to convert properties of lignocellulosic biomass for better use. |
| format | Article |
| id | doaj-art-1e7643a43648435c806762d9decec979 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-1e7643a43648435c806762d9decec9792025-02-02T12:33:15ZengNature PortfolioNature Communications2041-17232025-01-0116111410.1038/s41467-025-56626-yGlucomannan engineering highlights roles of galactosyl modification in fine-tuning cellulose-glucomannan interaction in Arabidopsis cell wallsYoshihisa Yoshimi0Li Yu1Rosalie Cresswell2Xinyu Guo3Alberto Echevarría-Poza4Jan J. Lyczakowski5Ray Dupree6Toshihisa Kotake7Paul Dupree8Department of Biochemistry, University of Cambridge, Hopkins Building, The Downing Site, Tennis Court RoadDepartment of Biochemistry, University of Cambridge, Hopkins Building, The Downing Site, Tennis Court RoadDepartment of Physics, University of WarwickDepartment of Biochemistry, University of Cambridge, Hopkins Building, The Downing Site, Tennis Court RoadDepartment of Biochemistry, University of Cambridge, Hopkins Building, The Downing Site, Tennis Court RoadDepartment of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian UniversityDepartment of Physics, University of WarwickDivision of Life Science, Graduate School of Science and Engineering, Saitama UniversityDepartment of Biochemistry, University of Cambridge, Hopkins Building, The Downing Site, Tennis Court RoadAbstract Widely found in most plant lineages, β-mannans are structurally diverse polysaccharides that can bind to cellulose fibrils to form the complex polysaccharide architecture of the cell wall. How changes in polysaccharide structure influence its cell wall solubility or promote appropriate interaction with cellulose fibrils is poorly understood. Glucomannan backbones acquire variable patterns of galactosyl substitutions, depending on plant developmental stage and species. Here, we show that fine-tuning of galactosyl modification on glucomannans is achieved by the differing acceptor recognition of mannan α-galactosyltransferases (MAGTs). Biochemical analysis and 13C solid-state nuclear magnetic resonance spectroscopy of Arabidopsis with cell wall glucomannan engineered by MAGTs reveal that the degree of galactosylation strongly affects the interaction with cellulose. The findings indicate that plants tailor galactosyl modification on glucomannans for constructing an appropriate cell wall architecture, paving the way to convert properties of lignocellulosic biomass for better use.https://doi.org/10.1038/s41467-025-56626-y |
| spellingShingle | Yoshihisa Yoshimi Li Yu Rosalie Cresswell Xinyu Guo Alberto Echevarría-Poza Jan J. Lyczakowski Ray Dupree Toshihisa Kotake Paul Dupree Glucomannan engineering highlights roles of galactosyl modification in fine-tuning cellulose-glucomannan interaction in Arabidopsis cell walls Nature Communications |
| title | Glucomannan engineering highlights roles of galactosyl modification in fine-tuning cellulose-glucomannan interaction in Arabidopsis cell walls |
| title_full | Glucomannan engineering highlights roles of galactosyl modification in fine-tuning cellulose-glucomannan interaction in Arabidopsis cell walls |
| title_fullStr | Glucomannan engineering highlights roles of galactosyl modification in fine-tuning cellulose-glucomannan interaction in Arabidopsis cell walls |
| title_full_unstemmed | Glucomannan engineering highlights roles of galactosyl modification in fine-tuning cellulose-glucomannan interaction in Arabidopsis cell walls |
| title_short | Glucomannan engineering highlights roles of galactosyl modification in fine-tuning cellulose-glucomannan interaction in Arabidopsis cell walls |
| title_sort | glucomannan engineering highlights roles of galactosyl modification in fine tuning cellulose glucomannan interaction in arabidopsis cell walls |
| url | https://doi.org/10.1038/s41467-025-56626-y |
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