In Vivo Antiplasmodial Activity of Terminalia mantaly Stem Bark Aqueous Extract in Mice Infected by Plasmodium berghei
Background. Terminalia mantaly is used in Cameroon traditional medicine to treat malaria and related symptoms. However, its antiplasmodial efficacy is still to be established. Objectives. The present study is aimed at evaluating the in vitro and in vivo antiplasmodial activity and the oral acute tox...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/4580526 |
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| author | Mariscal Brice Tchatat Tali Cedric Derick Jiatsa Mbouna Lauve Rachel Yamthe Tchokouaha Patrick Valere Tsouh Fokou Jaures Marius Tsakem Nangap Rodrigue Keumoe Alvine Ngoutane Mfopa Issakou Bakarnga-via Raceline Gounoue Kamkumo Fabrice Fekam Boyom |
| author_facet | Mariscal Brice Tchatat Tali Cedric Derick Jiatsa Mbouna Lauve Rachel Yamthe Tchokouaha Patrick Valere Tsouh Fokou Jaures Marius Tsakem Nangap Rodrigue Keumoe Alvine Ngoutane Mfopa Issakou Bakarnga-via Raceline Gounoue Kamkumo Fabrice Fekam Boyom |
| author_sort | Mariscal Brice Tchatat Tali |
| collection | DOAJ |
| description | Background. Terminalia mantaly is used in Cameroon traditional medicine to treat malaria and related symptoms. However, its antiplasmodial efficacy is still to be established. Objectives. The present study is aimed at evaluating the in vitro and in vivo antiplasmodial activity and the oral acute toxicity of the Terminalia mantaly extracts. Materials and Methods. Extracts were prepared from leaves and stem bark of T. mantaly, by maceration in distilled water, methanol, ethanol, dichloromethane (DCM), and hexane. All extracts were initially screened in vitro against the chloroquine-resistant strain W2 of P. falciparum to confirm its in vitro activity, and the most potent one was assessed in malaria mouse model at three concentrations (100, 200, and 400 mg/kg/bw). Biochemical, hematological, and histological parameters were also determined. Results. Overall, 7 extracts showed in vitro antiplasmodial activity with IC50 ranging from 0.809 μg/mL to 5.886 μg/mL. The aqueous extract from the stem bark of T. mantaly (Tmsbw) was the most potent (IC50=0.809 μg/mL) and was further assessed for acute toxicity and efficacy in Plasmodium berghei-infected mice. Tmsbw was safe in mice with a median lethal dose (LD50) higher than 2000 mg/kg of body weight. It also exerted a good antimalarial efficacy in vivo with ED50 of 69.50 mg/kg and had no significant effect on biochemical, hematological, and histological parameters. Conclusion. The results suggest that the stem bark extract of T. mantaly possesses antimalarial activity. |
| format | Article |
| id | doaj-art-1e574fe062974e17b44a870dd17a6d82 |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-1e574fe062974e17b44a870dd17a6d822025-02-03T05:44:11ZengWileyJournal of Parasitology Research2090-00232090-00312020-01-01202010.1155/2020/45805264580526In Vivo Antiplasmodial Activity of Terminalia mantaly Stem Bark Aqueous Extract in Mice Infected by Plasmodium bergheiMariscal Brice Tchatat Tali0Cedric Derick Jiatsa Mbouna1Lauve Rachel Yamthe Tchokouaha2Patrick Valere Tsouh Fokou3Jaures Marius Tsakem Nangap4Rodrigue Keumoe5Alvine Ngoutane Mfopa6Issakou Bakarnga-via7Raceline Gounoue Kamkumo8Fabrice Fekam Boyom9Antimicrobial & Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Study, Faculty of Science, University of Yaoundé 1, PO Box 812, Yaoundé, CameroonAntimicrobial & Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Study, Faculty of Science, University of Yaoundé 1, PO Box 812, Yaoundé, CameroonAntimicrobial & Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Study, Faculty of Science, University of Yaoundé 1, PO Box 812, Yaoundé, CameroonAntimicrobial & Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Study, Faculty of Science, University of Yaoundé 1, PO Box 812, Yaoundé, CameroonLaboratory of Animal Physiology, Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé 1, PO Box 812, Yaoundé, CameroonAntimicrobial & Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Study, Faculty of Science, University of Yaoundé 1, PO Box 812, Yaoundé, CameroonAntimicrobial & Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Study, Faculty of Science, University of Yaoundé 1, PO Box 812, Yaoundé, CameroonAntimicrobial & Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Study, Faculty of Science, University of Yaoundé 1, PO Box 812, Yaoundé, CameroonLaboratory of Animal Physiology, Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé 1, PO Box 812, Yaoundé, CameroonAntimicrobial & Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Study, Faculty of Science, University of Yaoundé 1, PO Box 812, Yaoundé, CameroonBackground. Terminalia mantaly is used in Cameroon traditional medicine to treat malaria and related symptoms. However, its antiplasmodial efficacy is still to be established. Objectives. The present study is aimed at evaluating the in vitro and in vivo antiplasmodial activity and the oral acute toxicity of the Terminalia mantaly extracts. Materials and Methods. Extracts were prepared from leaves and stem bark of T. mantaly, by maceration in distilled water, methanol, ethanol, dichloromethane (DCM), and hexane. All extracts were initially screened in vitro against the chloroquine-resistant strain W2 of P. falciparum to confirm its in vitro activity, and the most potent one was assessed in malaria mouse model at three concentrations (100, 200, and 400 mg/kg/bw). Biochemical, hematological, and histological parameters were also determined. Results. Overall, 7 extracts showed in vitro antiplasmodial activity with IC50 ranging from 0.809 μg/mL to 5.886 μg/mL. The aqueous extract from the stem bark of T. mantaly (Tmsbw) was the most potent (IC50=0.809 μg/mL) and was further assessed for acute toxicity and efficacy in Plasmodium berghei-infected mice. Tmsbw was safe in mice with a median lethal dose (LD50) higher than 2000 mg/kg of body weight. It also exerted a good antimalarial efficacy in vivo with ED50 of 69.50 mg/kg and had no significant effect on biochemical, hematological, and histological parameters. Conclusion. The results suggest that the stem bark extract of T. mantaly possesses antimalarial activity.http://dx.doi.org/10.1155/2020/4580526 |
| spellingShingle | Mariscal Brice Tchatat Tali Cedric Derick Jiatsa Mbouna Lauve Rachel Yamthe Tchokouaha Patrick Valere Tsouh Fokou Jaures Marius Tsakem Nangap Rodrigue Keumoe Alvine Ngoutane Mfopa Issakou Bakarnga-via Raceline Gounoue Kamkumo Fabrice Fekam Boyom In Vivo Antiplasmodial Activity of Terminalia mantaly Stem Bark Aqueous Extract in Mice Infected by Plasmodium berghei Journal of Parasitology Research |
| title | In Vivo Antiplasmodial Activity of Terminalia mantaly Stem Bark Aqueous Extract in Mice Infected by Plasmodium berghei |
| title_full | In Vivo Antiplasmodial Activity of Terminalia mantaly Stem Bark Aqueous Extract in Mice Infected by Plasmodium berghei |
| title_fullStr | In Vivo Antiplasmodial Activity of Terminalia mantaly Stem Bark Aqueous Extract in Mice Infected by Plasmodium berghei |
| title_full_unstemmed | In Vivo Antiplasmodial Activity of Terminalia mantaly Stem Bark Aqueous Extract in Mice Infected by Plasmodium berghei |
| title_short | In Vivo Antiplasmodial Activity of Terminalia mantaly Stem Bark Aqueous Extract in Mice Infected by Plasmodium berghei |
| title_sort | in vivo antiplasmodial activity of terminalia mantaly stem bark aqueous extract in mice infected by plasmodium berghei |
| url | http://dx.doi.org/10.1155/2020/4580526 |
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