Indirect Electrochemical Analysis of Crocin in Phytochemical Sample
A new electroanalytical method has been developed for the quantitative determination of crocin in a sample of stigmas of saffron (Crocus sativus L.). Crocin is polarographically inactive. However, cysteine in 0.02 M NaCl, pH=5.2±0.01 produces a well defined wave/peak with E1/2/Ep= –0.47/–0.45 V vs....
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2012/967079 |
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| author | Riyaz Ahmad Dar Pradeep Kumar Brahaman Sweety Tiwari Krishna Sadashiv Pitre |
| author_facet | Riyaz Ahmad Dar Pradeep Kumar Brahaman Sweety Tiwari Krishna Sadashiv Pitre |
| author_sort | Riyaz Ahmad Dar |
| collection | DOAJ |
| description | A new electroanalytical method has been developed for the quantitative determination of crocin in a sample of stigmas of saffron (Crocus sativus L.). Crocin is polarographically inactive. However, cysteine in 0.02 M NaCl, pH=5.2±0.01 produces a well defined wave/peak with E1/2/Ep= –0.47/–0.45 V vs. Ag/AgCl. On recording polarograms of a set of solution containing a fixed concentration of cysteine and varying concentrations of crocin under aforesaid experimental conditions a gradual decrease in peak height/diffusion current and a negative shift in peak potential was observed. Thus, indicating cysteine-crocin interaction. Amperometric titration indicated crocin to cysteine ratio of 1:2. The above amperometric titration procedure has been used to determine the concentration of crocin in a sample of saffron. Crystallization process was carried out for the extraction of crocin from dried powder of saffron stigmas. The crystallized crocin was identified by UV-Visible spectrophotometry(at 255 nm and 442 nm) and the quantitative analysis by the developed amperometric method. The concentration of crocin in saffron was found to be 2.13% and purity of isolated crocin 96.81%. The percent recovery varied from 98.56–100.31% and RSD (n=5) of 2.17%.The validation of the proposed procedure for the quantitative assay of crocin was examined via an evaluation of the repeatability, recovery, selectivity and relative standard deviation. |
| format | Article |
| id | doaj-art-1e513458823d4b4e930aca69764cf14c |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-1e513458823d4b4e930aca69764cf14c2025-02-03T01:02:33ZengWileyE-Journal of Chemistry0973-49452090-98102012-01-019291892510.1155/2012/967079Indirect Electrochemical Analysis of Crocin in Phytochemical SampleRiyaz Ahmad Dar0Pradeep Kumar Brahaman1Sweety Tiwari2Krishna Sadashiv Pitre3Chemical Technology Laboratory, Department of Chemistry, Dr. Hari Singh Gour University, Sagar-470003, Madhya Pradesh, IndiaChemical Technology Laboratory, Department of Chemistry, Dr. Hari Singh Gour University, Sagar-470003, Madhya Pradesh, IndiaChemical Technology Laboratory, Department of Chemistry, Dr. Hari Singh Gour University, Sagar-470003, Madhya Pradesh, IndiaChemical Technology Laboratory, Department of Chemistry, Dr. Hari Singh Gour University, Sagar-470003, Madhya Pradesh, IndiaA new electroanalytical method has been developed for the quantitative determination of crocin in a sample of stigmas of saffron (Crocus sativus L.). Crocin is polarographically inactive. However, cysteine in 0.02 M NaCl, pH=5.2±0.01 produces a well defined wave/peak with E1/2/Ep= –0.47/–0.45 V vs. Ag/AgCl. On recording polarograms of a set of solution containing a fixed concentration of cysteine and varying concentrations of crocin under aforesaid experimental conditions a gradual decrease in peak height/diffusion current and a negative shift in peak potential was observed. Thus, indicating cysteine-crocin interaction. Amperometric titration indicated crocin to cysteine ratio of 1:2. The above amperometric titration procedure has been used to determine the concentration of crocin in a sample of saffron. Crystallization process was carried out for the extraction of crocin from dried powder of saffron stigmas. The crystallized crocin was identified by UV-Visible spectrophotometry(at 255 nm and 442 nm) and the quantitative analysis by the developed amperometric method. The concentration of crocin in saffron was found to be 2.13% and purity of isolated crocin 96.81%. The percent recovery varied from 98.56–100.31% and RSD (n=5) of 2.17%.The validation of the proposed procedure for the quantitative assay of crocin was examined via an evaluation of the repeatability, recovery, selectivity and relative standard deviation.http://dx.doi.org/10.1155/2012/967079 |
| spellingShingle | Riyaz Ahmad Dar Pradeep Kumar Brahaman Sweety Tiwari Krishna Sadashiv Pitre Indirect Electrochemical Analysis of Crocin in Phytochemical Sample E-Journal of Chemistry |
| title | Indirect Electrochemical Analysis of Crocin in Phytochemical Sample |
| title_full | Indirect Electrochemical Analysis of Crocin in Phytochemical Sample |
| title_fullStr | Indirect Electrochemical Analysis of Crocin in Phytochemical Sample |
| title_full_unstemmed | Indirect Electrochemical Analysis of Crocin in Phytochemical Sample |
| title_short | Indirect Electrochemical Analysis of Crocin in Phytochemical Sample |
| title_sort | indirect electrochemical analysis of crocin in phytochemical sample |
| url | http://dx.doi.org/10.1155/2012/967079 |
| work_keys_str_mv | AT riyazahmaddar indirectelectrochemicalanalysisofcrocininphytochemicalsample AT pradeepkumarbrahaman indirectelectrochemicalanalysisofcrocininphytochemicalsample AT sweetytiwari indirectelectrochemicalanalysisofcrocininphytochemicalsample AT krishnasadashivpitre indirectelectrochemicalanalysisofcrocininphytochemicalsample |