Anti-inflammatory effect of Plantago asiatica crude extract in rat gout arthritis model
Plantago asiatica L., a perennial herb in the family Plantaginaceae, has been shown to impart several pharmacologic activities, including anti-oxidative, anti-inflammatory, and diuretic effects. In the study here, the anti-gout(y) arthritis (GA) effects of a crude extract from P. asiatica L. (PAE) w...
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Taylor & Francis Group
2025-12-01
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| Series: | Journal of Immunotoxicology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/1547691X.2025.2453156 |
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| author | Bingjun Qian Jun Hu Li Dai Yue Zhou Haixia Xu |
| author_facet | Bingjun Qian Jun Hu Li Dai Yue Zhou Haixia Xu |
| author_sort | Bingjun Qian |
| collection | DOAJ |
| description | Plantago asiatica L., a perennial herb in the family Plantaginaceae, has been shown to impart several pharmacologic activities, including anti-oxidative, anti-inflammatory, and diuretic effects. In the study here, the anti-gout(y) arthritis (GA) effects of a crude extract from P. asiatica L. (PAE) were investigated in a rat GA model. For this, PAE was prepared by ethanol extraction and analyzed for phytochemicals by RP-HPLC and Q-TOF-MS. Thereafter, potential therapeutic effects of the PAE were investigated in rats; Wistar rats (male, 8 wk-of-age) were randomly allocated into four groups (n = 9/group) and intra-articularly injected with 3 mg monosodium urate (MSU) in saline solution to establish a GA model. For the study, rats received oral dosings of 0.3 mg colchicine/kg or 1 g PAE/kg (w/w) before and after gout was established. At fixed times after the treatments, assessment of joint swelling ratios and pathological changes in the joints, as well as of select cytokine expression in the blood, was done. RP-HPLC results showed the PAE contained at least 8 ‘active’ ingredients, with plantamajoside, verbascoside, and cymaroside being the most abundant. In comparison to in control rats, MSU induced joint space narrowing, ankle joint swelling, and increased levels of pro-inflammatory interleukin (IL)-1β, IL-17a, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ, and reductions in anti-inflammatory IL-10 in the blood. PAE treatment significantly reversed patho- genic joint space narrowing and swelling, reversed the MSU-induced changes in inflammatory factors, and in general imparted effects very similar to those seen with colchicine (COL; known non-steroidal anti-inflammatory drug for clinical treatment of GA). Collectively, these findings provide experimental evidence supporting the potential applicability of PAE to treat gouty arthritis. |
| format | Article |
| id | doaj-art-1e4202debdec471087c1072798949a3c |
| institution | Kabale University |
| issn | 1547-691X 1547-6901 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
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| series | Journal of Immunotoxicology |
| spelling | doaj-art-1e4202debdec471087c1072798949a3c2025-02-03T07:53:59ZengTaylor & Francis GroupJournal of Immunotoxicology1547-691X1547-69012025-12-0122110.1080/1547691X.2025.2453156Anti-inflammatory effect of Plantago asiatica crude extract in rat gout arthritis modelBingjun Qian0Jun Hu1Li Dai2Yue Zhou3Haixia Xu4Department of Preventive Medicine, Institute of Biomedical Technology, Jiangsu Medical College, Yancheng, Jiangsu, ChinaDepartment of Preventive Medicine, Institute of Biomedical Technology, Jiangsu Medical College, Yancheng, Jiangsu, ChinaDepartment of Preventive Medicine, Institute of Biomedical Technology, Jiangsu Medical College, Yancheng, Jiangsu, ChinaDepartment of Nephrology, Nanjing First Hospital, Nanjing Medical University, Nanjing, ChinaSuzhou Catch Bio-Science and Technology Co. Ltd, Zhangjiagang, Jiangsu, ChinaPlantago asiatica L., a perennial herb in the family Plantaginaceae, has been shown to impart several pharmacologic activities, including anti-oxidative, anti-inflammatory, and diuretic effects. In the study here, the anti-gout(y) arthritis (GA) effects of a crude extract from P. asiatica L. (PAE) were investigated in a rat GA model. For this, PAE was prepared by ethanol extraction and analyzed for phytochemicals by RP-HPLC and Q-TOF-MS. Thereafter, potential therapeutic effects of the PAE were investigated in rats; Wistar rats (male, 8 wk-of-age) were randomly allocated into four groups (n = 9/group) and intra-articularly injected with 3 mg monosodium urate (MSU) in saline solution to establish a GA model. For the study, rats received oral dosings of 0.3 mg colchicine/kg or 1 g PAE/kg (w/w) before and after gout was established. At fixed times after the treatments, assessment of joint swelling ratios and pathological changes in the joints, as well as of select cytokine expression in the blood, was done. RP-HPLC results showed the PAE contained at least 8 ‘active’ ingredients, with plantamajoside, verbascoside, and cymaroside being the most abundant. In comparison to in control rats, MSU induced joint space narrowing, ankle joint swelling, and increased levels of pro-inflammatory interleukin (IL)-1β, IL-17a, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ, and reductions in anti-inflammatory IL-10 in the blood. PAE treatment significantly reversed patho- genic joint space narrowing and swelling, reversed the MSU-induced changes in inflammatory factors, and in general imparted effects very similar to those seen with colchicine (COL; known non-steroidal anti-inflammatory drug for clinical treatment of GA). Collectively, these findings provide experimental evidence supporting the potential applicability of PAE to treat gouty arthritis.https://www.tandfonline.com/doi/10.1080/1547691X.2025.2453156Plantain extractphytochemicalsacute gout arthritisanti-inflammatoryIL-1β |
| spellingShingle | Bingjun Qian Jun Hu Li Dai Yue Zhou Haixia Xu Anti-inflammatory effect of Plantago asiatica crude extract in rat gout arthritis model Journal of Immunotoxicology Plantain extract phytochemicals acute gout arthritis anti-inflammatory IL-1β |
| title | Anti-inflammatory effect of Plantago asiatica crude extract in rat gout arthritis model |
| title_full | Anti-inflammatory effect of Plantago asiatica crude extract in rat gout arthritis model |
| title_fullStr | Anti-inflammatory effect of Plantago asiatica crude extract in rat gout arthritis model |
| title_full_unstemmed | Anti-inflammatory effect of Plantago asiatica crude extract in rat gout arthritis model |
| title_short | Anti-inflammatory effect of Plantago asiatica crude extract in rat gout arthritis model |
| title_sort | anti inflammatory effect of plantago asiatica crude extract in rat gout arthritis model |
| topic | Plantain extract phytochemicals acute gout arthritis anti-inflammatory IL-1β |
| url | https://www.tandfonline.com/doi/10.1080/1547691X.2025.2453156 |
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