Nutrient Stimulation of Indigenous Microorganisms for Oil-in-Water Emulsion in a Medium Temperature Petroleum Reservoir with Ca2+-Rich Brine
One of the challenges indigenous microbial enhanced oil recovery (MEOR) is facing is the high percentage of divalent ions, which obstruct the growth and metabolism of microorganisms and destabilize the oil-in-water (o/w) emulsion. Six formulas were selected for the stimulation of indigenous microbes...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Geofluids |
| Online Access: | http://dx.doi.org/10.1155/2021/6659038 |
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| author | Guoxin Shi Yuanyuan Jia Lei Bai Guoqiang Li Xiaoli Liu Meichen Liu Xuecheng Dai Xuefeng Tian Hongbo Wang Ting Ma |
| author_facet | Guoxin Shi Yuanyuan Jia Lei Bai Guoqiang Li Xiaoli Liu Meichen Liu Xuecheng Dai Xuefeng Tian Hongbo Wang Ting Ma |
| author_sort | Guoxin Shi |
| collection | DOAJ |
| description | One of the challenges indigenous microbial enhanced oil recovery (MEOR) is facing is the high percentage of divalent ions, which obstruct the growth and metabolism of microorganisms and destabilize the oil-in-water (o/w) emulsion. Six formulas were selected for the stimulation of indigenous microbes and to compare their performances on the oil emulsification and oil spreading in the Luliang oilfield containing Ca2+-rich brine. Illumina MiSeq sequencing of 16S rRNA genes was applied to investigate the structural response of microbial communities to various formulas. The results showed that the addition of proper organic phosphorus and the optimal P/N ratio (0.01) can facilitate production of biosurfactant and create stable o/w emulsion with specific reservoir condition containing Ca2+-rich brine, through direct stimulation of certain functional microbes. This study provides a promising path for direct enrichment of biosurfactant-producing and oil-degrading Dietzia genus and a potential instructional approach of indigenous MEOR in Luliang oilfield. |
| format | Article |
| id | doaj-art-1e32133c2e8541758973f884d3096616 |
| institution | Kabale University |
| issn | 1468-8115 1468-8123 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Geofluids |
| spelling | doaj-art-1e32133c2e8541758973f884d30966162025-02-03T01:04:37ZengWileyGeofluids1468-81151468-81232021-01-01202110.1155/2021/66590386659038Nutrient Stimulation of Indigenous Microorganisms for Oil-in-Water Emulsion in a Medium Temperature Petroleum Reservoir with Ca2+-Rich BrineGuoxin Shi0Yuanyuan Jia1Lei Bai2Guoqiang Li3Xiaoli Liu4Meichen Liu5Xuecheng Dai6Xuefeng Tian7Hongbo Wang8Ting Ma9Experimental Inspection Research Institute, Xinjiang Oilfield Co. Ltd., Karamay 834000, ChinaKey Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaExperimental Inspection Research Institute, Xinjiang Oilfield Co. Ltd., Karamay 834000, ChinaKey Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaExperimental Inspection Research Institute, Xinjiang Oilfield Co. Ltd., Karamay 834000, ChinaKey Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaExperimental Inspection Research Institute, Xinjiang Oilfield Co. Ltd., Karamay 834000, ChinaKey Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaExperimental Inspection Research Institute, Xinjiang Oilfield Co. Ltd., Karamay 834000, ChinaKey Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaOne of the challenges indigenous microbial enhanced oil recovery (MEOR) is facing is the high percentage of divalent ions, which obstruct the growth and metabolism of microorganisms and destabilize the oil-in-water (o/w) emulsion. Six formulas were selected for the stimulation of indigenous microbes and to compare their performances on the oil emulsification and oil spreading in the Luliang oilfield containing Ca2+-rich brine. Illumina MiSeq sequencing of 16S rRNA genes was applied to investigate the structural response of microbial communities to various formulas. The results showed that the addition of proper organic phosphorus and the optimal P/N ratio (0.01) can facilitate production of biosurfactant and create stable o/w emulsion with specific reservoir condition containing Ca2+-rich brine, through direct stimulation of certain functional microbes. This study provides a promising path for direct enrichment of biosurfactant-producing and oil-degrading Dietzia genus and a potential instructional approach of indigenous MEOR in Luliang oilfield.http://dx.doi.org/10.1155/2021/6659038 |
| spellingShingle | Guoxin Shi Yuanyuan Jia Lei Bai Guoqiang Li Xiaoli Liu Meichen Liu Xuecheng Dai Xuefeng Tian Hongbo Wang Ting Ma Nutrient Stimulation of Indigenous Microorganisms for Oil-in-Water Emulsion in a Medium Temperature Petroleum Reservoir with Ca2+-Rich Brine Geofluids |
| title | Nutrient Stimulation of Indigenous Microorganisms for Oil-in-Water Emulsion in a Medium Temperature Petroleum Reservoir with Ca2+-Rich Brine |
| title_full | Nutrient Stimulation of Indigenous Microorganisms for Oil-in-Water Emulsion in a Medium Temperature Petroleum Reservoir with Ca2+-Rich Brine |
| title_fullStr | Nutrient Stimulation of Indigenous Microorganisms for Oil-in-Water Emulsion in a Medium Temperature Petroleum Reservoir with Ca2+-Rich Brine |
| title_full_unstemmed | Nutrient Stimulation of Indigenous Microorganisms for Oil-in-Water Emulsion in a Medium Temperature Petroleum Reservoir with Ca2+-Rich Brine |
| title_short | Nutrient Stimulation of Indigenous Microorganisms for Oil-in-Water Emulsion in a Medium Temperature Petroleum Reservoir with Ca2+-Rich Brine |
| title_sort | nutrient stimulation of indigenous microorganisms for oil in water emulsion in a medium temperature petroleum reservoir with ca2 rich brine |
| url | http://dx.doi.org/10.1155/2021/6659038 |
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