Transcriptomics Analysis of Maize (<i>Zea mays</i> L.) in Response to the Infection by <i>Setosphaeria turcica</i>
Northern corn leaf blight (NCLB), caused by <i>Setosphaeria turcica</i> (<i>S. turcica</i>), is one of the devastating foliar diseases of maize (<i>Zea mays</i>) in maize-producing regions globally. Previous research has predominantly centered on elucidating the i...
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2024-12-01
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| author | Hui Jia Pan Li Bu Tao Yuwei Liu Zhihang Liu Mengfang Zhu He Zhou Maocun Wang Jingao Dong Shouqin Gu Xiaodong Gong |
| author_facet | Hui Jia Pan Li Bu Tao Yuwei Liu Zhihang Liu Mengfang Zhu He Zhou Maocun Wang Jingao Dong Shouqin Gu Xiaodong Gong |
| author_sort | Hui Jia |
| collection | DOAJ |
| description | Northern corn leaf blight (NCLB), caused by <i>Setosphaeria turcica</i> (<i>S. turcica</i>), is one of the devastating foliar diseases of maize (<i>Zea mays</i>) in maize-producing regions globally. Previous research has predominantly centered on elucidating the infection strategy and process of the pathogen, but the molecular mechanism of maize response to the pathogen is still largely unknown. In this study, we employed transcriptomics technology to comprehensively analyze alterations in RNA expression profiles within maize leaves at critical time points (hours post-infestation, 24 hpi, and 72 hpi) during <i>S. turcica</i> infection. Our study identified 7196 differentially expressed genes (DEGs) involved in the maize leaf response to <i>S. turcica</i> infection compared to the control (CK at 0 hpi). Functional analysis revealed that these DEGs were enriched in multiple metabolic pathways. Notably, genes associated with “benzoxazinone biosynthesis”, “tetracyclic pyrrole biosynthesis”, and “photosynthesis” were all down-regulated. In contrast, DEGs related to “phenol metabolism” and “phenylpropanoid metabolism” were significantly upregulated. Moreover, the genes belonging to the NAC, MYB-related, HB, and WRKY transcription factor families were also significantly enriched among the DEGs. The expression levels of six randomly selected DEGs were validated using qRT-PCR, confirming the accuracy of the RNA-Seq findings. This study delves into the functional genes and metabolic pathways closely associated with maize’s response to <i>S. turcica</i> infection, providing foundational data for a deeper understanding of the molecular mechanisms underlying the interaction between <i>S. turcica</i> and maize. |
| format | Article |
| id | doaj-art-1e2e2ddd64224451bf54be30c3ea9154 |
| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj-art-1e2e2ddd64224451bf54be30c3ea91542025-01-24T13:16:35ZengMDPI AGAgronomy2073-43952024-12-011516910.3390/agronomy15010069Transcriptomics Analysis of Maize (<i>Zea mays</i> L.) in Response to the Infection by <i>Setosphaeria turcica</i>Hui Jia0Pan Li1Bu Tao2Yuwei Liu3Zhihang Liu4Mengfang Zhu5He Zhou6Maocun Wang7Jingao Dong8Shouqin Gu9Xiaodong Gong10State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding 071000, ChinaCollege of Plant Protection, Hebei Agricultural University, Baoding 071000, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding 071000, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding 071000, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding 071000, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding 071000, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding 071000, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, ChinaNorthern corn leaf blight (NCLB), caused by <i>Setosphaeria turcica</i> (<i>S. turcica</i>), is one of the devastating foliar diseases of maize (<i>Zea mays</i>) in maize-producing regions globally. Previous research has predominantly centered on elucidating the infection strategy and process of the pathogen, but the molecular mechanism of maize response to the pathogen is still largely unknown. In this study, we employed transcriptomics technology to comprehensively analyze alterations in RNA expression profiles within maize leaves at critical time points (hours post-infestation, 24 hpi, and 72 hpi) during <i>S. turcica</i> infection. Our study identified 7196 differentially expressed genes (DEGs) involved in the maize leaf response to <i>S. turcica</i> infection compared to the control (CK at 0 hpi). Functional analysis revealed that these DEGs were enriched in multiple metabolic pathways. Notably, genes associated with “benzoxazinone biosynthesis”, “tetracyclic pyrrole biosynthesis”, and “photosynthesis” were all down-regulated. In contrast, DEGs related to “phenol metabolism” and “phenylpropanoid metabolism” were significantly upregulated. Moreover, the genes belonging to the NAC, MYB-related, HB, and WRKY transcription factor families were also significantly enriched among the DEGs. The expression levels of six randomly selected DEGs were validated using qRT-PCR, confirming the accuracy of the RNA-Seq findings. This study delves into the functional genes and metabolic pathways closely associated with maize’s response to <i>S. turcica</i> infection, providing foundational data for a deeper understanding of the molecular mechanisms underlying the interaction between <i>S. turcica</i> and maize.https://www.mdpi.com/2073-4395/15/1/69<i>Zea mays</i><i>Setosphaeria turcica</i>RNA-Seqdifferentially expressed genesenrichment analysis |
| spellingShingle | Hui Jia Pan Li Bu Tao Yuwei Liu Zhihang Liu Mengfang Zhu He Zhou Maocun Wang Jingao Dong Shouqin Gu Xiaodong Gong Transcriptomics Analysis of Maize (<i>Zea mays</i> L.) in Response to the Infection by <i>Setosphaeria turcica</i> Agronomy <i>Zea mays</i> <i>Setosphaeria turcica</i> RNA-Seq differentially expressed genes enrichment analysis |
| title | Transcriptomics Analysis of Maize (<i>Zea mays</i> L.) in Response to the Infection by <i>Setosphaeria turcica</i> |
| title_full | Transcriptomics Analysis of Maize (<i>Zea mays</i> L.) in Response to the Infection by <i>Setosphaeria turcica</i> |
| title_fullStr | Transcriptomics Analysis of Maize (<i>Zea mays</i> L.) in Response to the Infection by <i>Setosphaeria turcica</i> |
| title_full_unstemmed | Transcriptomics Analysis of Maize (<i>Zea mays</i> L.) in Response to the Infection by <i>Setosphaeria turcica</i> |
| title_short | Transcriptomics Analysis of Maize (<i>Zea mays</i> L.) in Response to the Infection by <i>Setosphaeria turcica</i> |
| title_sort | transcriptomics analysis of maize i zea mays i l in response to the infection by i setosphaeria turcica i |
| topic | <i>Zea mays</i> <i>Setosphaeria turcica</i> RNA-Seq differentially expressed genes enrichment analysis |
| url | https://www.mdpi.com/2073-4395/15/1/69 |
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