Optimization of Saccharomyces cerevisiae medium by response surface methodology
In order to improve the yield of Saccharomyces cerevisiae, using dry mass of S. cerevisiae as the response value, on the basis of single factor tests, the formula of S. cerevisiae medium was optimized by Plackett Burman tests, steepest climbing tests and response surface methodology. The results sho...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
Editorial Department of China Brewing
2024-06-01
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| Series: | Zhongguo niangzao |
| Subjects: | |
| Online Access: | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-6-206.pdf |
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| Summary: | In order to improve the yield of Saccharomyces cerevisiae, using dry mass of S. cerevisiae as the response value, on the basis of single factor tests, the formula of S. cerevisiae medium was optimized by Plackett Burman tests, steepest climbing tests and response surface methodology. The results showed that glucose, yeast extract and ammonium sulfate were selected as the main factors affecting the dry mass of yeast by Plackett Burman tests. Finally, the optimal fermentation medium determined by response surface methodology was as follows: glucose 48 g/L, yeast extract 38 g/L, ammonium sulfate 2 g/L, dipotassium hydrogen phosphate 3 g/L, calcium chloride 0.1 g/L, zinc sulfate 0.05 g/L, manganese sulfate 0.05 g/L, VB<sub>1</sub> 0.1 g/L, and VB<sub>7</sub> 0.1 g/L. Under the optimal medium combination, the dry cell mass of S. cerevisiae reached 22.31 g/L, which was 3.31 times of that before optimization. |
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| ISSN: | 0254-5071 |