Continuous Drip Flow System to Develop Biofilm of E. faecalis under Anaerobic Conditions
Purpose. To evaluate a structurally mature E. faecalis biofilm developed under anaerobic/dynamic conditions in an in vitro system. Methods. An experimental device was developed using a continuous drip flow system designed to develop biofilm under anaerobic conditions. The inoculum was replaced every...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/706189 |
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| author | Ana Maria Gonzalez Erika Corpus Amaury Pozos-Guillen Daniel Silva-Herzog Antonio Aragon-Piña Nestor Cohenca |
| author_facet | Ana Maria Gonzalez Erika Corpus Amaury Pozos-Guillen Daniel Silva-Herzog Antonio Aragon-Piña Nestor Cohenca |
| author_sort | Ana Maria Gonzalez |
| collection | DOAJ |
| description | Purpose. To evaluate a structurally mature E. faecalis biofilm developed under anaerobic/dynamic conditions in an in vitro system. Methods. An experimental device was developed using a continuous drip flow system designed to develop biofilm under anaerobic conditions. The inoculum was replaced every 24 hours with a fresh growth medium for up to 10 days to feed the system. Gram staining was done every 24 hours to control the microorganism purity. Biofilms developed under the system were evaluated under the scanning electron microscope (SEM). Results. SEM micrographs demonstrated mushroom-shaped structures, corresponding to a mature E. faecalis biofilm. In the mature biofilm bacterial cells are totally encased in a polymeric extracellular matrix. Conclusions. The proposed in vitro system model provides an additional useful tool to study the biofilm concept in endodontic microbiology, allowing for a better understanding of persistent root canal infections. |
| format | Article |
| id | doaj-art-1dfb097769444718961d51d730248d11 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-1dfb097769444718961d51d730248d112025-02-03T01:23:01ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/706189706189Continuous Drip Flow System to Develop Biofilm of E. faecalis under Anaerobic ConditionsAna Maria Gonzalez0Erika Corpus1Amaury Pozos-Guillen2Daniel Silva-Herzog3Antonio Aragon-Piña4Nestor Cohenca5Endodontics Postgraduate Program, Faculty of Dentistry, The Autonomous University of San Luis Potosí, Manuel Nava 2, 78290 San Luis Potosí, SLP, MexicoEndodontics Postgraduate Program, Faculty of Dentistry, The Autonomous University of San Luis Potosí, Manuel Nava 2, 78290 San Luis Potosí, SLP, MexicoBasic Sciences Laboratory, Faculty of Dentistry, The Autonomous University of San Luis Potosí, Manuel Nava 2, 78290 San Luis Potosí, SLP, MexicoEndodontics Postgraduate Program, Faculty of Dentistry, The Autonomous University of San Luis Potosí, Manuel Nava 2, 78290 San Luis Potosí, SLP, MexicoFaculty of Engineering, The Autonomous University of San Luis Potosí, Manuel Nava 8, 78290 San Luis Potosí, SLP, MexicoDepartment of Endodontics and Department of Pediatric Dentistry, School of Dentistry, University of Washington, 6222 NE 74th Street, Seattle, 98115 WA, USAPurpose. To evaluate a structurally mature E. faecalis biofilm developed under anaerobic/dynamic conditions in an in vitro system. Methods. An experimental device was developed using a continuous drip flow system designed to develop biofilm under anaerobic conditions. The inoculum was replaced every 24 hours with a fresh growth medium for up to 10 days to feed the system. Gram staining was done every 24 hours to control the microorganism purity. Biofilms developed under the system were evaluated under the scanning electron microscope (SEM). Results. SEM micrographs demonstrated mushroom-shaped structures, corresponding to a mature E. faecalis biofilm. In the mature biofilm bacterial cells are totally encased in a polymeric extracellular matrix. Conclusions. The proposed in vitro system model provides an additional useful tool to study the biofilm concept in endodontic microbiology, allowing for a better understanding of persistent root canal infections.http://dx.doi.org/10.1155/2014/706189 |
| spellingShingle | Ana Maria Gonzalez Erika Corpus Amaury Pozos-Guillen Daniel Silva-Herzog Antonio Aragon-Piña Nestor Cohenca Continuous Drip Flow System to Develop Biofilm of E. faecalis under Anaerobic Conditions The Scientific World Journal |
| title | Continuous Drip Flow System to Develop Biofilm of E. faecalis under Anaerobic Conditions |
| title_full | Continuous Drip Flow System to Develop Biofilm of E. faecalis under Anaerobic Conditions |
| title_fullStr | Continuous Drip Flow System to Develop Biofilm of E. faecalis under Anaerobic Conditions |
| title_full_unstemmed | Continuous Drip Flow System to Develop Biofilm of E. faecalis under Anaerobic Conditions |
| title_short | Continuous Drip Flow System to Develop Biofilm of E. faecalis under Anaerobic Conditions |
| title_sort | continuous drip flow system to develop biofilm of e faecalis under anaerobic conditions |
| url | http://dx.doi.org/10.1155/2014/706189 |
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