The DEAD-Box RNA Helicase DDX3 Interacts with m6A RNA Demethylase ALKBH5
DDX3 is a member of the family of DEAD-box RNA helicases. DDX3 is a multifaceted helicase and plays essential roles in key biological processes such as cell cycle, stress response, apoptosis, and RNA metabolism. In this study, we found that DDX3 interacted with ALKBH5, an m6A RNA demethylase. The AT...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/8596135 |
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| _version_ | 1832562168587878400 |
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| author | Abdullah Shah Farooq Rashid Hassaan Mehboob Awan Shanshan Hu Xiaolin Wang Liang Chen Ge Shan |
| author_facet | Abdullah Shah Farooq Rashid Hassaan Mehboob Awan Shanshan Hu Xiaolin Wang Liang Chen Ge Shan |
| author_sort | Abdullah Shah |
| collection | DOAJ |
| description | DDX3 is a member of the family of DEAD-box RNA helicases. DDX3 is a multifaceted helicase and plays essential roles in key biological processes such as cell cycle, stress response, apoptosis, and RNA metabolism. In this study, we found that DDX3 interacted with ALKBH5, an m6A RNA demethylase. The ATP domain of DDX3 and DSBH domain of ALKBH5 were indispensable to their interaction with each other. Furthermore, DDX3 could modulate the demethylation of mRNAs. We also showed that DDX3 regulated the methylation status of microRNAs and there was an interaction between DDX3 and AGO2. The dynamics of m6A RNA modification is still a field demanding further investigation, and here, we add a link by showing that RNA demethylation can be regulated by proteins such as DDX3. |
| format | Article |
| id | doaj-art-1dd4a986837943aea80ab804d9ec2ae9 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-1dd4a986837943aea80ab804d9ec2ae92025-02-03T01:23:15ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/85961358596135The DEAD-Box RNA Helicase DDX3 Interacts with m6A RNA Demethylase ALKBH5Abdullah Shah0Farooq Rashid1Hassaan Mehboob Awan2Shanshan Hu3Xiaolin Wang4Liang Chen5Ge Shan6CAS Key Laboratory of Innate Immunity and Chronic Disease, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, University of Science and Technology of China, Hefei, ChinaCAS Key Laboratory of Innate Immunity and Chronic Disease, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, University of Science and Technology of China, Hefei, ChinaCAS Key Laboratory of Innate Immunity and Chronic Disease, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, University of Science and Technology of China, Hefei, ChinaAnhui Province Key Laboratory of Brain Function and Brain Disease, Hefei, Anhui 230001, ChinaCAS Key Laboratory of Innate Immunity and Chronic Disease, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, University of Science and Technology of China, Hefei, ChinaCAS Key Laboratory of Innate Immunity and Chronic Disease, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, University of Science and Technology of China, Hefei, ChinaCAS Key Laboratory of Innate Immunity and Chronic Disease, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, University of Science and Technology of China, Hefei, ChinaDDX3 is a member of the family of DEAD-box RNA helicases. DDX3 is a multifaceted helicase and plays essential roles in key biological processes such as cell cycle, stress response, apoptosis, and RNA metabolism. In this study, we found that DDX3 interacted with ALKBH5, an m6A RNA demethylase. The ATP domain of DDX3 and DSBH domain of ALKBH5 were indispensable to their interaction with each other. Furthermore, DDX3 could modulate the demethylation of mRNAs. We also showed that DDX3 regulated the methylation status of microRNAs and there was an interaction between DDX3 and AGO2. The dynamics of m6A RNA modification is still a field demanding further investigation, and here, we add a link by showing that RNA demethylation can be regulated by proteins such as DDX3.http://dx.doi.org/10.1155/2017/8596135 |
| spellingShingle | Abdullah Shah Farooq Rashid Hassaan Mehboob Awan Shanshan Hu Xiaolin Wang Liang Chen Ge Shan The DEAD-Box RNA Helicase DDX3 Interacts with m6A RNA Demethylase ALKBH5 Stem Cells International |
| title | The DEAD-Box RNA Helicase DDX3 Interacts with m6A RNA Demethylase ALKBH5 |
| title_full | The DEAD-Box RNA Helicase DDX3 Interacts with m6A RNA Demethylase ALKBH5 |
| title_fullStr | The DEAD-Box RNA Helicase DDX3 Interacts with m6A RNA Demethylase ALKBH5 |
| title_full_unstemmed | The DEAD-Box RNA Helicase DDX3 Interacts with m6A RNA Demethylase ALKBH5 |
| title_short | The DEAD-Box RNA Helicase DDX3 Interacts with m6A RNA Demethylase ALKBH5 |
| title_sort | dead box rna helicase ddx3 interacts with m6a rna demethylase alkbh5 |
| url | http://dx.doi.org/10.1155/2017/8596135 |
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