Tensile force promotes osteogenic differentiation via ephrinB2-EphB4 signaling pathway in orthodontic tooth movement
Abstract Objective To investigating whether osteogenic differentiation of osteoblasts promoted by tension force (TF) is mediated by ephrinB2-EphB4 signaling. Methods TF was applied to MC3T3-E1 cells, then CCK-8 and live/dead staining were used to detect cell proliferation. Levels of osteogenic diffe...
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BMC
2025-01-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-025-05491-8 |
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| author | Hang Yu Xiaoxi Wei Huan Jiang Huichuan Qi Yi Zhang Min Hu |
| author_facet | Hang Yu Xiaoxi Wei Huan Jiang Huichuan Qi Yi Zhang Min Hu |
| author_sort | Hang Yu |
| collection | DOAJ |
| description | Abstract Objective To investigating whether osteogenic differentiation of osteoblasts promoted by tension force (TF) is mediated by ephrinB2-EphB4 signaling. Methods TF was applied to MC3T3-E1 cells, then CCK-8 and live/dead staining were used to detect cell proliferation. Levels of osteogenic differentiation-related factors were detected by ALP staining, ARS staining, qPCR and western blot. NVP-BHG712 was used to block EphB4 receptor. Establishing a rat orthodontic tooth movement (OTM) model, ephrinB2-Fc and NVP-BHG712 were used to treat rats. Micro-CT and H&E staining were used to detect alveolar bone. Changes of MAPK pathways were detected to investigate whether they were downstream of ephrinB2-EphB4 signaling in mediating TF promote osteogenic differentiation. Result We explored the effect of TF on MC3T3-E1 cells, and found that TF significantly promoted osteogenic differentiation, but when EphB4 receptor was blocked, the promotion was inhibited. In vivo, we found that TF improved alveolar bone formation through ephrinB2-EphB4 signaling. Further investigation into the signaling pathways revealed that TF significantly increased levels of MAPK pathways, however, when EphB4 receptor was blocked, only the promotion of p-ERK1/2 was decreased. Conclusion TF promotes osteogenic differentiation through ephrinB2-EphB4 signaling and ERK1/2 pathway is a downstream of ephrinB2-EphB4 signaling partially mediate mediates TF-induced promotion of osteogenic differentiation. |
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| institution | Kabale University |
| issn | 1472-6831 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
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| series | BMC Oral Health |
| spelling | doaj-art-1dcc2bc4f8d54fe3896f20a3f2a50e992025-01-26T12:55:20ZengBMCBMC Oral Health1472-68312025-01-0125111310.1186/s12903-025-05491-8Tensile force promotes osteogenic differentiation via ephrinB2-EphB4 signaling pathway in orthodontic tooth movementHang Yu0Xiaoxi Wei1Huan Jiang2Huichuan Qi3Yi Zhang4Min Hu5Department of Orthodontics, Stomatology School of Jilin UniversityDepartment of Orthodontics, Stomatology School of Jilin UniversityDepartment of Orthodontics, Stomatology School of Jilin UniversityDepartment of Orthodontics, Stomatology School of Jilin UniversityDepartment of Orthodontics, Stomatology School of Jilin UniversityDepartment of Orthodontics, Stomatology School of Jilin UniversityAbstract Objective To investigating whether osteogenic differentiation of osteoblasts promoted by tension force (TF) is mediated by ephrinB2-EphB4 signaling. Methods TF was applied to MC3T3-E1 cells, then CCK-8 and live/dead staining were used to detect cell proliferation. Levels of osteogenic differentiation-related factors were detected by ALP staining, ARS staining, qPCR and western blot. NVP-BHG712 was used to block EphB4 receptor. Establishing a rat orthodontic tooth movement (OTM) model, ephrinB2-Fc and NVP-BHG712 were used to treat rats. Micro-CT and H&E staining were used to detect alveolar bone. Changes of MAPK pathways were detected to investigate whether they were downstream of ephrinB2-EphB4 signaling in mediating TF promote osteogenic differentiation. Result We explored the effect of TF on MC3T3-E1 cells, and found that TF significantly promoted osteogenic differentiation, but when EphB4 receptor was blocked, the promotion was inhibited. In vivo, we found that TF improved alveolar bone formation through ephrinB2-EphB4 signaling. Further investigation into the signaling pathways revealed that TF significantly increased levels of MAPK pathways, however, when EphB4 receptor was blocked, only the promotion of p-ERK1/2 was decreased. Conclusion TF promotes osteogenic differentiation through ephrinB2-EphB4 signaling and ERK1/2 pathway is a downstream of ephrinB2-EphB4 signaling partially mediate mediates TF-induced promotion of osteogenic differentiation.https://doi.org/10.1186/s12903-025-05491-8Orthodontic tooth movementTensile forceOsteogenic differentiationephrinB2-EphB4ERK1/2 |
| spellingShingle | Hang Yu Xiaoxi Wei Huan Jiang Huichuan Qi Yi Zhang Min Hu Tensile force promotes osteogenic differentiation via ephrinB2-EphB4 signaling pathway in orthodontic tooth movement BMC Oral Health Orthodontic tooth movement Tensile force Osteogenic differentiation ephrinB2-EphB4 ERK1/2 |
| title | Tensile force promotes osteogenic differentiation via ephrinB2-EphB4 signaling pathway in orthodontic tooth movement |
| title_full | Tensile force promotes osteogenic differentiation via ephrinB2-EphB4 signaling pathway in orthodontic tooth movement |
| title_fullStr | Tensile force promotes osteogenic differentiation via ephrinB2-EphB4 signaling pathway in orthodontic tooth movement |
| title_full_unstemmed | Tensile force promotes osteogenic differentiation via ephrinB2-EphB4 signaling pathway in orthodontic tooth movement |
| title_short | Tensile force promotes osteogenic differentiation via ephrinB2-EphB4 signaling pathway in orthodontic tooth movement |
| title_sort | tensile force promotes osteogenic differentiation via ephrinb2 ephb4 signaling pathway in orthodontic tooth movement |
| topic | Orthodontic tooth movement Tensile force Osteogenic differentiation ephrinB2-EphB4 ERK1/2 |
| url | https://doi.org/10.1186/s12903-025-05491-8 |
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