Antihyperglycemic Activity of Eucalyptus tereticornis in Insulin-Resistant Cells and a Nutritional Model of Diabetic Mice
Eucalyptus tereticornis is a plant used in traditional medicine to control diabetes, but this effect has not been proved scientifically. Here, we demonstrated through in vitro assays that E. tereticornis extracts increase glucose uptake and inhibit their production in insulin-resistant C2C12 and Hep...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Advances in Pharmacological Sciences |
| Online Access: | http://dx.doi.org/10.1155/2015/418673 |
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| author | Alis Guillén Sergio Granados Kevin Eduardo Rivas Omar Estrada Luis Fernando Echeverri Norman Balcázar |
| author_facet | Alis Guillén Sergio Granados Kevin Eduardo Rivas Omar Estrada Luis Fernando Echeverri Norman Balcázar |
| author_sort | Alis Guillén |
| collection | DOAJ |
| description | Eucalyptus tereticornis is a plant used in traditional medicine to control diabetes, but this effect has not been proved scientifically. Here, we demonstrated through in vitro assays that E. tereticornis extracts increase glucose uptake and inhibit their production in insulin-resistant C2C12 and HepG2 cells, respectively. Furthermore, in a nutritional model using diabetic mice, the administration of ethyl acetate extract of E. tereticornis reduced fasting glycaemia, improved tolerance to glucose, and reduced resistance to insulin. Likewise, this extract had anti-inflammatory effects in adipose tissue when compared to control diabetic mice. Via bioguided assays and sequential purification of the crude extract, a triterpenoid-rich fraction from ethyl acetate extracts was shown to be responsible for the biological activity. Similarly, we identified the main compound responsible for the antihyperglycemic activity in this extract. This study shows that triterpenes found in E. tereticornis extracts act as hypoglycemic/antidiabetic compounds and contribute to the understanding of their use in traditional medicine. |
| format | Article |
| id | doaj-art-1d9f81a13c2d49b4af7cd32e2ef01261 |
| institution | Kabale University |
| issn | 1687-6334 1687-6342 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Pharmacological Sciences |
| spelling | doaj-art-1d9f81a13c2d49b4af7cd32e2ef012612025-02-03T06:44:22ZengWileyAdvances in Pharmacological Sciences1687-63341687-63422015-01-01201510.1155/2015/418673418673Antihyperglycemic Activity of Eucalyptus tereticornis in Insulin-Resistant Cells and a Nutritional Model of Diabetic MiceAlis Guillén0Sergio Granados1Kevin Eduardo Rivas2Omar Estrada3Luis Fernando Echeverri4Norman Balcázar5Molecular Genetics Group, University of Antioquia, Calle 70, No. 52-21, A.A. 1226, Medellín, ColombiaDepartment of Physiology and Biochemistry, School of Medicine, University of Antioquia, Calle 70, No. 52-21, A.A. 1226, Medellín, ColombiaMolecular Genetics Group, University of Antioquia, Calle 70, No. 52-21, A.A. 1226, Medellín, ColombiaLaboratory of Cellular Physiology, Center of Biophysic and Biochemistry, IVIC, Carretera Panamericana km 11, Altos de Pipe, Caracas, VenezuelaGroup of Organic Natural Product Chemistry, Faculty of Natural and Exact Sciences, University of Antioquia, Calle 70, No. 52-21, A.A. 1226, Medellín, ColombiaMolecular Genetics Group, University of Antioquia, Calle 70, No. 52-21, A.A. 1226, Medellín, ColombiaEucalyptus tereticornis is a plant used in traditional medicine to control diabetes, but this effect has not been proved scientifically. Here, we demonstrated through in vitro assays that E. tereticornis extracts increase glucose uptake and inhibit their production in insulin-resistant C2C12 and HepG2 cells, respectively. Furthermore, in a nutritional model using diabetic mice, the administration of ethyl acetate extract of E. tereticornis reduced fasting glycaemia, improved tolerance to glucose, and reduced resistance to insulin. Likewise, this extract had anti-inflammatory effects in adipose tissue when compared to control diabetic mice. Via bioguided assays and sequential purification of the crude extract, a triterpenoid-rich fraction from ethyl acetate extracts was shown to be responsible for the biological activity. Similarly, we identified the main compound responsible for the antihyperglycemic activity in this extract. This study shows that triterpenes found in E. tereticornis extracts act as hypoglycemic/antidiabetic compounds and contribute to the understanding of their use in traditional medicine.http://dx.doi.org/10.1155/2015/418673 |
| spellingShingle | Alis Guillén Sergio Granados Kevin Eduardo Rivas Omar Estrada Luis Fernando Echeverri Norman Balcázar Antihyperglycemic Activity of Eucalyptus tereticornis in Insulin-Resistant Cells and a Nutritional Model of Diabetic Mice Advances in Pharmacological Sciences |
| title | Antihyperglycemic Activity of Eucalyptus tereticornis in Insulin-Resistant Cells and a Nutritional Model of Diabetic Mice |
| title_full | Antihyperglycemic Activity of Eucalyptus tereticornis in Insulin-Resistant Cells and a Nutritional Model of Diabetic Mice |
| title_fullStr | Antihyperglycemic Activity of Eucalyptus tereticornis in Insulin-Resistant Cells and a Nutritional Model of Diabetic Mice |
| title_full_unstemmed | Antihyperglycemic Activity of Eucalyptus tereticornis in Insulin-Resistant Cells and a Nutritional Model of Diabetic Mice |
| title_short | Antihyperglycemic Activity of Eucalyptus tereticornis in Insulin-Resistant Cells and a Nutritional Model of Diabetic Mice |
| title_sort | antihyperglycemic activity of eucalyptus tereticornis in insulin resistant cells and a nutritional model of diabetic mice |
| url | http://dx.doi.org/10.1155/2015/418673 |
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