Molecular Characterization of BsCu/Zn-Superoxide Dismutases and BsMn-Superoxide Dismutases from Chinese Black Sleeper (<i>Bostrychus sinensis</i>)
Superoxide dismutases (SODs) are critical antioxidant enzymes that protect organisms by catalyzing the dismutation of superoxide radicals into molecular oxygen (O<sub>2</sub>) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). In this study, we cloned the full-le...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
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| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/15/5/653 |
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| Summary: | Superoxide dismutases (SODs) are critical antioxidant enzymes that protect organisms by catalyzing the dismutation of superoxide radicals into molecular oxygen (O<sub>2</sub>) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). In this study, we cloned the full-length cDNA of two SODs (BsCu/Zn-SOD and BsMn-SOD) from the Chinese black sleeper. The cDNA of BsCu/Zn-SOD consists of 465 nucleotides encoding 154 amino acids, while the BsMn-SOD comprises 678 nucleotides encoding 225 amino acids. The PCR results indicated that in normal tissues, the highest expression of BsCu/Zn-SOD (1.02-fold) was primarily observed in muscle, whereas the highest expression of BsMn-SOD (6.42-fold) was noted in skin. Following treatment with <i>Vibrio parahaemolyticus</i>, the highest expression of BsCu/Zn-SOD (3.67-fold) was detected in the spleen, while BsMn-SOD (3.36-fold) presented the highest expression in the blood. After poly(I:C) treatment, the highest expression of BsCu/Zn-SOD (2.92-fold) was found in the blood, with BsMn-SOD (2.37-fold) showing the highest expression in the spleen. Following Cd<sup>2+</sup> treatment, the highest expression of BsCu/Zn-SOD (3.36-fold) was recorded in the liver at the 1/2 96 h LC50 concentration, and in the spleen at both the 1/4 96 h LC50 (2.50-fold) and 1/8 96 h LC50 concentrations. In contrast, BsMn-SOD presented the highest expression (2.60-fold) in the liver across all three concentrations. These findings suggest that both BsCu/Zn-SOD and BsMn-SOD play vital roles in the innate immune response to environmental challenges, laying the foundation for further exploration of antioxidant mechanisms. |
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| ISSN: | 2076-2615 |