Influence of Epinastine Hydrochloride, an H1-Receptor Antagonist, on the Function of Mite Allergen-Pulsed Murine Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo
There is established concept that dendritic cells (DCs) play essential roles in the development of allergic immune responses. However, the influence of H1 receptor antagonists on DC functions is not well defined. The aim of the present study was to examine the effect of epinastine hydrochloride (EP)...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2009-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2009/738038 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832551590152634368 |
|---|---|
| author | Ken-Zaburo Oshima Kazuhito Asano Ken-Ichi Kanai Miyuki Suzuki Harumi Suzaki |
| author_facet | Ken-Zaburo Oshima Kazuhito Asano Ken-Ichi Kanai Miyuki Suzuki Harumi Suzaki |
| author_sort | Ken-Zaburo Oshima |
| collection | DOAJ |
| description | There is established concept that dendritic cells (DCs) play essential roles in the development of allergic immune responses. However, the influence of H1 receptor antagonists on DC functions is not well defined. The aim of the present study was to examine the effect of epinastine hydrochloride (EP), the most notable histamine H1 receptor antagonists in Japan, on Dermatophagoides farinae (Der f)-pulsed mouse bone marrow-derived DCs in vitro and in vivo. EP at more than 25 ng/mL could significantly inhibit the production of IL-6, TNF-𝛼 and IL-10 from Der f-pulsed DCs, which was increased by Der f challenge in vitro. On the other hand, EP increased the ability of Der f-pulsed DCs to produce IL-12. Intranasal instillation of Der f-pulsed DCs resulted in nasal eosinophilia associated with a significant increase in IL-5 levels in nasal lavage fluids. Der f-pulsed and EP-treated DCs significantly inhibited nasal eosinophila and reduced IL-5. These results indicate that EP inhibits the development of Th2 immune responses through the modulation of DC functions and results in favorable modification of clinical status of allergic diseases. |
| format | Article |
| id | doaj-art-1d752d174c91494c90513a3e551b6f3c |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-1d752d174c91494c90513a3e551b6f3c2025-02-03T06:01:01ZengWileyMediators of Inflammation0962-93511466-18612009-01-01200910.1155/2009/738038738038Influence of Epinastine Hydrochloride, an H1-Receptor Antagonist, on the Function of Mite Allergen-Pulsed Murine Bone Marrow-Derived Dendritic Cells In Vitro and In VivoKen-Zaburo Oshima0Kazuhito Asano1Ken-Ichi Kanai2Miyuki Suzuki3Harumi Suzaki4Department of Otolaryngology, School of Medicine, Showa University, Tokyo 142-8555, JapanDivision of Physiology, School of Nursing and Rehabilitation Sciences, Showa University, Yokohama 226-8555, JapanDepartment of Otolaryngology, School of Medicine, Showa University, Tokyo 142-8555, JapanDepartment of Otolaryngology, School of Medicine, Showa University, Tokyo 142-8555, JapanDepartment of Otolaryngology, School of Medicine, Showa University, Tokyo 142-8555, JapanThere is established concept that dendritic cells (DCs) play essential roles in the development of allergic immune responses. However, the influence of H1 receptor antagonists on DC functions is not well defined. The aim of the present study was to examine the effect of epinastine hydrochloride (EP), the most notable histamine H1 receptor antagonists in Japan, on Dermatophagoides farinae (Der f)-pulsed mouse bone marrow-derived DCs in vitro and in vivo. EP at more than 25 ng/mL could significantly inhibit the production of IL-6, TNF-𝛼 and IL-10 from Der f-pulsed DCs, which was increased by Der f challenge in vitro. On the other hand, EP increased the ability of Der f-pulsed DCs to produce IL-12. Intranasal instillation of Der f-pulsed DCs resulted in nasal eosinophilia associated with a significant increase in IL-5 levels in nasal lavage fluids. Der f-pulsed and EP-treated DCs significantly inhibited nasal eosinophila and reduced IL-5. These results indicate that EP inhibits the development of Th2 immune responses through the modulation of DC functions and results in favorable modification of clinical status of allergic diseases.http://dx.doi.org/10.1155/2009/738038 |
| spellingShingle | Ken-Zaburo Oshima Kazuhito Asano Ken-Ichi Kanai Miyuki Suzuki Harumi Suzaki Influence of Epinastine Hydrochloride, an H1-Receptor Antagonist, on the Function of Mite Allergen-Pulsed Murine Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo Mediators of Inflammation |
| title | Influence of Epinastine Hydrochloride, an H1-Receptor Antagonist, on the Function of Mite Allergen-Pulsed Murine Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo |
| title_full | Influence of Epinastine Hydrochloride, an H1-Receptor Antagonist, on the Function of Mite Allergen-Pulsed Murine Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo |
| title_fullStr | Influence of Epinastine Hydrochloride, an H1-Receptor Antagonist, on the Function of Mite Allergen-Pulsed Murine Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo |
| title_full_unstemmed | Influence of Epinastine Hydrochloride, an H1-Receptor Antagonist, on the Function of Mite Allergen-Pulsed Murine Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo |
| title_short | Influence of Epinastine Hydrochloride, an H1-Receptor Antagonist, on the Function of Mite Allergen-Pulsed Murine Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo |
| title_sort | influence of epinastine hydrochloride an h1 receptor antagonist on the function of mite allergen pulsed murine bone marrow derived dendritic cells in vitro and in vivo |
| url | http://dx.doi.org/10.1155/2009/738038 |
| work_keys_str_mv | AT kenzaburooshima influenceofepinastinehydrochlorideanh1receptorantagonistonthefunctionofmiteallergenpulsedmurinebonemarrowderiveddendriticcellsinvitroandinvivo AT kazuhitoasano influenceofepinastinehydrochlorideanh1receptorantagonistonthefunctionofmiteallergenpulsedmurinebonemarrowderiveddendriticcellsinvitroandinvivo AT kenichikanai influenceofepinastinehydrochlorideanh1receptorantagonistonthefunctionofmiteallergenpulsedmurinebonemarrowderiveddendriticcellsinvitroandinvivo AT miyukisuzuki influenceofepinastinehydrochlorideanh1receptorantagonistonthefunctionofmiteallergenpulsedmurinebonemarrowderiveddendriticcellsinvitroandinvivo AT harumisuzaki influenceofepinastinehydrochlorideanh1receptorantagonistonthefunctionofmiteallergenpulsedmurinebonemarrowderiveddendriticcellsinvitroandinvivo |