Characterisation of IGF-1 (rs7136446) and IL-6 (rs1800795) polymorphisms among breast cancer patients in western Algeria
Breast cancer (BC) is the most frequently diagnosed cancer among women worldwide, including Algeria. Certain single nucleotide polymorphisms (SNPs) have been linked to higher risk of BC. Several studies have been performed on the insulin- like growth factor 1 (IGF-1) T > C (rs7136446) and the int...
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2024-10-01
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| Series: | Polish Journal of Pathology |
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| Online Access: | https://www.termedia.pl/Characterisation-of-IGF-1-rs7136446-and-IL-6-rs1800795-polymorphisms-among-breast-cancer-patients-in-western-Algeria,55,54825,1,1.html |
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| author | Fatima Zohraa Boussouf Asmahane Medjdoub Zineb Tahari Moussa Messatfa Noria Bouras Chahinez Amira Dahmani Sonia Amal Seddiki Tewfik Sahraoui |
| author_facet | Fatima Zohraa Boussouf Asmahane Medjdoub Zineb Tahari Moussa Messatfa Noria Bouras Chahinez Amira Dahmani Sonia Amal Seddiki Tewfik Sahraoui |
| author_sort | Fatima Zohraa Boussouf |
| collection | DOAJ |
| description | Breast cancer (BC) is the most frequently diagnosed cancer among women worldwide, including Algeria. Certain single nucleotide polymorphisms (SNPs) have been linked to higher risk of BC. Several studies have been performed on the insulin- like growth factor 1 (IGF-1) T > C (rs7136446) and the interleukin 6 (IL-6) 174 G > C (rs1800795) SNPs to explore their role in BC. The aim of this study is to investigate the association between the 2 SNPs, IL-6 (rs1800795) and IGF-1 (rs7136446) with BC in the population of western Algeria.
This study was carried out for the first time among the Algerian population. This case-control study included 109 BC patients and 112 healthy controls. Genomic DNA was extracted from peripheral blood samples. DNA concentration was determined using the Qubit 2.0 fluorometer. The genotyping of selected SNPs was performed by real-time polymerase chain reaction followed by statistical analysis to assess genotypic frequencies and genetic association with BC.
The results showed that IGF-1 rs7136446 was positively associated with BC (p = 0.00001) and that the distribution of genotype frequencies of rs7136446 showed a statistically difference between human epidermal growth factor receptor 2 (HER-2) positive and HER-2 negative BC (p = 0.04), but this association did not last after the correction of Bonferroni. No association was found in genotype distribution of the IL-6 (rs1800795) among controls and BC patients or with clinicopathological parameters including HER-2 status in BC (p > 0.05).
In summary, our findings indicate that IGF-1 rs7136446 is associated with BC in our population of western Algeria. |
| format | Article |
| id | doaj-art-1d6b7c6456f84ccd83da3f634950697b |
| institution | Kabale University |
| issn | 1233-9687 2084-9869 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Polish Journal of Pathology |
| spelling | doaj-art-1d6b7c6456f84ccd83da3f634950697b2025-01-27T11:36:07ZengTermedia Publishing HousePolish Journal of Pathology1233-96872084-98692024-10-0175320521410.5114/pjp.2024.14323054825Characterisation of IGF-1 (rs7136446) and IL-6 (rs1800795) polymorphisms among breast cancer patients in western AlgeriaFatima Zohraa BoussoufAsmahane MedjdoubZineb TahariMoussa MessatfaNoria BourasChahinez Amira DahmaniSonia Amal SeddikiTewfik SahraouiBreast cancer (BC) is the most frequently diagnosed cancer among women worldwide, including Algeria. Certain single nucleotide polymorphisms (SNPs) have been linked to higher risk of BC. Several studies have been performed on the insulin- like growth factor 1 (IGF-1) T > C (rs7136446) and the interleukin 6 (IL-6) 174 G > C (rs1800795) SNPs to explore their role in BC. The aim of this study is to investigate the association between the 2 SNPs, IL-6 (rs1800795) and IGF-1 (rs7136446) with BC in the population of western Algeria. This study was carried out for the first time among the Algerian population. This case-control study included 109 BC patients and 112 healthy controls. Genomic DNA was extracted from peripheral blood samples. DNA concentration was determined using the Qubit 2.0 fluorometer. The genotyping of selected SNPs was performed by real-time polymerase chain reaction followed by statistical analysis to assess genotypic frequencies and genetic association with BC. The results showed that IGF-1 rs7136446 was positively associated with BC (p = 0.00001) and that the distribution of genotype frequencies of rs7136446 showed a statistically difference between human epidermal growth factor receptor 2 (HER-2) positive and HER-2 negative BC (p = 0.04), but this association did not last after the correction of Bonferroni. No association was found in genotype distribution of the IL-6 (rs1800795) among controls and BC patients or with clinicopathological parameters including HER-2 status in BC (p > 0.05). In summary, our findings indicate that IGF-1 rs7136446 is associated with BC in our population of western Algeria.https://www.termedia.pl/Characterisation-of-IGF-1-rs7136446-and-IL-6-rs1800795-polymorphisms-among-breast-cancer-patients-in-western-Algeria,55,54825,1,1.htmlbreast cancer (bc) interleukin 6 (il-6) insulin-like growth factor 1 (igf-1) human epidermal growth factor receptor 2 (her-2) single nucleotide polymorphisms (snps) western algeria. |
| spellingShingle | Fatima Zohraa Boussouf Asmahane Medjdoub Zineb Tahari Moussa Messatfa Noria Bouras Chahinez Amira Dahmani Sonia Amal Seddiki Tewfik Sahraoui Characterisation of IGF-1 (rs7136446) and IL-6 (rs1800795) polymorphisms among breast cancer patients in western Algeria Polish Journal of Pathology breast cancer (bc) interleukin 6 (il-6) insulin-like growth factor 1 (igf-1) human epidermal growth factor receptor 2 (her-2) single nucleotide polymorphisms (snps) western algeria. |
| title | Characterisation of IGF-1 (rs7136446) and IL-6 (rs1800795) polymorphisms among breast cancer patients in western Algeria |
| title_full | Characterisation of IGF-1 (rs7136446) and IL-6 (rs1800795) polymorphisms among breast cancer patients in western Algeria |
| title_fullStr | Characterisation of IGF-1 (rs7136446) and IL-6 (rs1800795) polymorphisms among breast cancer patients in western Algeria |
| title_full_unstemmed | Characterisation of IGF-1 (rs7136446) and IL-6 (rs1800795) polymorphisms among breast cancer patients in western Algeria |
| title_short | Characterisation of IGF-1 (rs7136446) and IL-6 (rs1800795) polymorphisms among breast cancer patients in western Algeria |
| title_sort | characterisation of igf 1 rs7136446 and il 6 rs1800795 polymorphisms among breast cancer patients in western algeria |
| topic | breast cancer (bc) interleukin 6 (il-6) insulin-like growth factor 1 (igf-1) human epidermal growth factor receptor 2 (her-2) single nucleotide polymorphisms (snps) western algeria. |
| url | https://www.termedia.pl/Characterisation-of-IGF-1-rs7136446-and-IL-6-rs1800795-polymorphisms-among-breast-cancer-patients-in-western-Algeria,55,54825,1,1.html |
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