Matrine relieved DHAV-1-induced hepatocyte excessive interferon and pyroptosis by activating mitophagy
Duck hepatitis A virus type 1 (DHAV-1) is a significant pathogen affecting ducklings, capable of causing rapid mortality and adversely impacting the development of the duck industry. Matrine, the primary active ingredient in various Chinese herbal medicines, has demonstrated antiviral and anti-infla...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124011799 |
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| author | Weiran Wang Xiang Fu Bolin Gu Mengxin Hu Jiaguo Liu |
| author_facet | Weiran Wang Xiang Fu Bolin Gu Mengxin Hu Jiaguo Liu |
| author_sort | Weiran Wang |
| collection | DOAJ |
| description | Duck hepatitis A virus type 1 (DHAV-1) is a significant pathogen affecting ducklings, capable of causing rapid mortality and adversely impacting the development of the duck industry. Matrine, the primary active ingredient in various Chinese herbal medicines, has demonstrated antiviral and anti-inflammatory properties. Nevertheless, the effects and mechanisms of action of matrine against DHAV-1 infection remain unclear. This research investigates the effects of matrine on DHAV-1 infection and elucidates the mechanisms involved. We found that matrine mitigated the excessive retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) signaling response, pyroptosis, and mitochondrial damage induced by DHAV-1 in duckling livers and duck embryonic hepatocytes (DEHs). Additionally, by incorporating the autophagy inhibitor chloroquine, we observed that the effects of matrine on the regulation of excessive interferon (IFN) production, pyroptosis, mitochondrial damage, and oxidative stress were reversed. Overall, matrine inhibited excessive IFN production and pyroptosis by promoting mitophagy, suggesting that matrine may act as a possible therapeutic agent for addressing DHAV-1 infection and other viral hepatitis. |
| format | Article |
| id | doaj-art-1d59cbbbe6b04cbda9618477b636d9a2 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-1d59cbbbe6b04cbda9618477b636d9a22025-01-22T05:40:39ZengElsevierPoultry Science0032-57912025-01-011041104601Matrine relieved DHAV-1-induced hepatocyte excessive interferon and pyroptosis by activating mitophagyWeiran Wang0Xiang Fu1Bolin Gu2Mengxin Hu3Jiaguo Liu4MOE Joint International Research Laboratory of Animal Health and Food Safety and Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety and Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety and Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety and Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR ChinaCorresponding author.; MOE Joint International Research Laboratory of Animal Health and Food Safety and Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR ChinaDuck hepatitis A virus type 1 (DHAV-1) is a significant pathogen affecting ducklings, capable of causing rapid mortality and adversely impacting the development of the duck industry. Matrine, the primary active ingredient in various Chinese herbal medicines, has demonstrated antiviral and anti-inflammatory properties. Nevertheless, the effects and mechanisms of action of matrine against DHAV-1 infection remain unclear. This research investigates the effects of matrine on DHAV-1 infection and elucidates the mechanisms involved. We found that matrine mitigated the excessive retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) signaling response, pyroptosis, and mitochondrial damage induced by DHAV-1 in duckling livers and duck embryonic hepatocytes (DEHs). Additionally, by incorporating the autophagy inhibitor chloroquine, we observed that the effects of matrine on the regulation of excessive interferon (IFN) production, pyroptosis, mitochondrial damage, and oxidative stress were reversed. Overall, matrine inhibited excessive IFN production and pyroptosis by promoting mitophagy, suggesting that matrine may act as a possible therapeutic agent for addressing DHAV-1 infection and other viral hepatitis.http://www.sciencedirect.com/science/article/pii/S0032579124011799DHAV-1MatrineRLR signalingPyroptosisMitophagy |
| spellingShingle | Weiran Wang Xiang Fu Bolin Gu Mengxin Hu Jiaguo Liu Matrine relieved DHAV-1-induced hepatocyte excessive interferon and pyroptosis by activating mitophagy Poultry Science DHAV-1 Matrine RLR signaling Pyroptosis Mitophagy |
| title | Matrine relieved DHAV-1-induced hepatocyte excessive interferon and pyroptosis by activating mitophagy |
| title_full | Matrine relieved DHAV-1-induced hepatocyte excessive interferon and pyroptosis by activating mitophagy |
| title_fullStr | Matrine relieved DHAV-1-induced hepatocyte excessive interferon and pyroptosis by activating mitophagy |
| title_full_unstemmed | Matrine relieved DHAV-1-induced hepatocyte excessive interferon and pyroptosis by activating mitophagy |
| title_short | Matrine relieved DHAV-1-induced hepatocyte excessive interferon and pyroptosis by activating mitophagy |
| title_sort | matrine relieved dhav 1 induced hepatocyte excessive interferon and pyroptosis by activating mitophagy |
| topic | DHAV-1 Matrine RLR signaling Pyroptosis Mitophagy |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124011799 |
| work_keys_str_mv | AT weiranwang matrinerelieveddhav1inducedhepatocyteexcessiveinterferonandpyroptosisbyactivatingmitophagy AT xiangfu matrinerelieveddhav1inducedhepatocyteexcessiveinterferonandpyroptosisbyactivatingmitophagy AT bolingu matrinerelieveddhav1inducedhepatocyteexcessiveinterferonandpyroptosisbyactivatingmitophagy AT mengxinhu matrinerelieveddhav1inducedhepatocyteexcessiveinterferonandpyroptosisbyactivatingmitophagy AT jiaguoliu matrinerelieveddhav1inducedhepatocyteexcessiveinterferonandpyroptosisbyactivatingmitophagy |