Fetal indusium griseum is a possible biomarker of the regularity of brain midline development in 3T MR imaging: A retrospective observational study

Abstract Introduction This study aimed to assess the visibility of the indusium griseum (IG) in magnetic resonance (MR) scans of the human fetal brain and to evaluate its reliability as an imaging biomarker of the normality of brain midline development. Material and methods The retrospective observa...

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Main Authors: Ivana Pogledic, Mihaela Bobić‐Rasonja, Christian Mitter, Andrija Štajduhar, Ernst Schwartz, Marija Milković‐Periša, Pascal A. Baltzer, Maarten Lequin, Elisabeth Krampl‐Bettelheim, Gregor Kasprian, Miloš Judaš, Daniela Prayer, Natasa Jovanov‐Milosevic
Format: Article
Language:English
Published: Wiley 2024-05-01
Series:Acta Obstetricia et Gynecologica Scandinavica
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Online Access:https://doi.org/10.1111/aogs.14781
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author Ivana Pogledic
Mihaela Bobić‐Rasonja
Christian Mitter
Andrija Štajduhar
Ernst Schwartz
Marija Milković‐Periša
Pascal A. Baltzer
Maarten Lequin
Elisabeth Krampl‐Bettelheim
Gregor Kasprian
Miloš Judaš
Daniela Prayer
Natasa Jovanov‐Milosevic
author_facet Ivana Pogledic
Mihaela Bobić‐Rasonja
Christian Mitter
Andrija Štajduhar
Ernst Schwartz
Marija Milković‐Periša
Pascal A. Baltzer
Maarten Lequin
Elisabeth Krampl‐Bettelheim
Gregor Kasprian
Miloš Judaš
Daniela Prayer
Natasa Jovanov‐Milosevic
author_sort Ivana Pogledic
collection DOAJ
description Abstract Introduction This study aimed to assess the visibility of the indusium griseum (IG) in magnetic resonance (MR) scans of the human fetal brain and to evaluate its reliability as an imaging biomarker of the normality of brain midline development. Material and methods The retrospective observational study encompassed T2‐w 3T MR images from 90 post‐mortem fetal brains and immunohistochemical sections from 41 fetal brains (16–40 gestational weeks) without cerebral pathology. Three raters independently inspected and evaluated the visibility of IG in post‐mortem and in vivo MR scans. Weighted kappa statistics and regression analysis were used to determine inter‐ and intra‐rater agreement and the type and strength of the association of IG visibility with gestational age. Results The visibility of the IG was the highest between the 25 and 30 gestational week period, with a very good inter‐rater variability (kappa 0.623–0.709) and excellent intra‐rater variability (kappa 0.81–0.93). The immunochemical analysis of the histoarchitecture of IG discloses the expression of highly hydrated extracellular molecules in IG as the substrate of higher signal intensity and best visibility of IG during the mid‐fetal period. Conclusions The knowledge of developmental brain histology and fetal age allows us to predict the IG‐visibility in magnetic resonance imaging (MRI) and use it as a biomarker to evaluate the morphogenesis of the brain midline. As a biomarker, IG is significant for post‐mortem pathological examination by MRI. Therefore, in the clinical in vivo imaging examination, IG should be anticipated when an assessment of the brain midline structures is needed in mid‐gestation, including corpus callosum thickness measurements.
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spelling doaj-art-1d46b529d0c5440cbd54cf37e5305f8d2025-08-20T02:09:34ZengWileyActa Obstetricia et Gynecologica Scandinavica0001-63491600-04122024-05-01103589790610.1111/aogs.14781Fetal indusium griseum is a possible biomarker of the regularity of brain midline development in 3T MR imaging: A retrospective observational studyIvana Pogledic0Mihaela Bobić‐Rasonja1Christian Mitter2Andrija Štajduhar3Ernst Schwartz4Marija Milković‐Periša5Pascal A. Baltzer6Maarten Lequin7Elisabeth Krampl‐Bettelheim8Gregor Kasprian9Miloš Judaš10Daniela Prayer11Natasa Jovanov‐Milosevic12Division of Neuroradiology and Musculoskeletal Radiology, Department of Biomedical Imaging and Image‐guided Therapy, Allgemeines Krankenhaus Medical University of Vienna Vienna AustriaDepartment of Biology School of Medicine, University of Zagreb Zagreb CroatiaDivision of Neuroradiology and Musculoskeletal Radiology, Department of Biomedical Imaging and Image‐guided Therapy, Allgemeines Krankenhaus Medical University of Vienna Vienna AustriaCroatian Institute for Brain Research, Scientific Center of Excellence for Basic, Clinical and Translational Neuroscience School of Medicine University of Zagreb Zagreb CroatiaComputational Imaging Research Lab, Department of Biomedical Imaging and Image‐Guided Therapy Medical University of Vienna Vienna AustriaDepartment of Pathology and Cytology, School of Medicine University Hospital Center Zagreb, University of Zagreb Zagreb CroatiaDivision of Neuroradiology and Musculoskeletal Radiology, Department of Biomedical Imaging and Image‐guided Therapy, Allgemeines Krankenhaus Medical University of Vienna Vienna AustriaDepartment of Radiology University Medical Center Utrecht Utrecht The NetherlandsDepartment of Obstetrics and Feto‐maternal Medicine Medical University of Vienna Vienna AustriaDivision of Neuroradiology and Musculoskeletal Radiology, Department of Biomedical Imaging and Image‐guided Therapy, Allgemeines Krankenhaus Medical University of Vienna Vienna AustriaCroatian Institute for Brain Research, Scientific Center of Excellence for Basic, Clinical and Translational Neuroscience School of Medicine University of Zagreb Zagreb CroatiaDivision of Neuroradiology and Musculoskeletal Radiology, Department of Biomedical Imaging and Image‐guided Therapy, Allgemeines Krankenhaus Medical University of Vienna Vienna AustriaDepartment of Biology School of Medicine, University of Zagreb Zagreb CroatiaAbstract Introduction This study aimed to assess the visibility of the indusium griseum (IG) in magnetic resonance (MR) scans of the human fetal brain and to evaluate its reliability as an imaging biomarker of the normality of brain midline development. Material and methods The retrospective observational study encompassed T2‐w 3T MR images from 90 post‐mortem fetal brains and immunohistochemical sections from 41 fetal brains (16–40 gestational weeks) without cerebral pathology. Three raters independently inspected and evaluated the visibility of IG in post‐mortem and in vivo MR scans. Weighted kappa statistics and regression analysis were used to determine inter‐ and intra‐rater agreement and the type and strength of the association of IG visibility with gestational age. Results The visibility of the IG was the highest between the 25 and 30 gestational week period, with a very good inter‐rater variability (kappa 0.623–0.709) and excellent intra‐rater variability (kappa 0.81–0.93). The immunochemical analysis of the histoarchitecture of IG discloses the expression of highly hydrated extracellular molecules in IG as the substrate of higher signal intensity and best visibility of IG during the mid‐fetal period. Conclusions The knowledge of developmental brain histology and fetal age allows us to predict the IG‐visibility in magnetic resonance imaging (MRI) and use it as a biomarker to evaluate the morphogenesis of the brain midline. As a biomarker, IG is significant for post‐mortem pathological examination by MRI. Therefore, in the clinical in vivo imaging examination, IG should be anticipated when an assessment of the brain midline structures is needed in mid‐gestation, including corpus callosum thickness measurements.https://doi.org/10.1111/aogs.14781corpus callosumextracellular matrixindusium griseummalformations of cortical developmentprenatal diagnosissubplate
spellingShingle Ivana Pogledic
Mihaela Bobić‐Rasonja
Christian Mitter
Andrija Štajduhar
Ernst Schwartz
Marija Milković‐Periša
Pascal A. Baltzer
Maarten Lequin
Elisabeth Krampl‐Bettelheim
Gregor Kasprian
Miloš Judaš
Daniela Prayer
Natasa Jovanov‐Milosevic
Fetal indusium griseum is a possible biomarker of the regularity of brain midline development in 3T MR imaging: A retrospective observational study
Acta Obstetricia et Gynecologica Scandinavica
corpus callosum
extracellular matrix
indusium griseum
malformations of cortical development
prenatal diagnosis
subplate
title Fetal indusium griseum is a possible biomarker of the regularity of brain midline development in 3T MR imaging: A retrospective observational study
title_full Fetal indusium griseum is a possible biomarker of the regularity of brain midline development in 3T MR imaging: A retrospective observational study
title_fullStr Fetal indusium griseum is a possible biomarker of the regularity of brain midline development in 3T MR imaging: A retrospective observational study
title_full_unstemmed Fetal indusium griseum is a possible biomarker of the regularity of brain midline development in 3T MR imaging: A retrospective observational study
title_short Fetal indusium griseum is a possible biomarker of the regularity of brain midline development in 3T MR imaging: A retrospective observational study
title_sort fetal indusium griseum is a possible biomarker of the regularity of brain midline development in 3t mr imaging a retrospective observational study
topic corpus callosum
extracellular matrix
indusium griseum
malformations of cortical development
prenatal diagnosis
subplate
url https://doi.org/10.1111/aogs.14781
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