Identification of cytoplasm types in accessions of the family Brassicaceae (Brassicaceae Burnett) with DNA markers
Identification of cytoplasm types with the use of molecular markers enables to simplify the parent line pair selection for hybrid development based on CMS. Forty accessions of the family Brassicaceae (Brassicaceae Burnett) were analyzed with 22 pairs of PCR primers taken from previous research. Seve...
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2015-12-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/445 |
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| author | E. A. Domblides A. S. Domblides T. V. Zayachkovskaya L. L. Bondareva |
| author_facet | E. A. Domblides A. S. Domblides T. V. Zayachkovskaya L. L. Bondareva |
| author_sort | E. A. Domblides |
| collection | DOAJ |
| description | Identification of cytoplasm types with the use of molecular markers enables to simplify the parent line pair selection for hybrid development based on CMS. Forty accessions of the family Brassicaceae (Brassicaceae Burnett) were analyzed with 22 pairs of PCR primers taken from previous research. Seven types of cytoplasm (Ogura, Ogu-NWSUAF, nap, pol, cam, rad, ole) were observed among breeding accessions using standard and multiplex PCR technique. The sequence of PCR products that differentiated different types of sterile cytoplasm confirmed the presence of three genes, orf138, orf222, and orf224, in mitochondrial DNA associated with exhibition of CMS. The accession of Nappa cabbage (Brassica rapa Pekinensis Group) had two genes, orf138 and orf222, which corresponded to cytoplasm Ogu-NWSUAF. All accessions carrying Ogura cytoplasm had the 417 bp fragment, which was 100 % identical to the mitochondrial orf138 gene sequence corres- ponding to Type А (formerly nine sequence Types, from A to I, had been identified). An exception was the accession of white head cabbage Tekila F1 with a new allelic variant of orf138 gene, which combined the 39-bp deletion typical of the Type F orf138 sequence and two nonsynonymous substitutions, A→T and G→A, at positions 95 and 99, respectively. |
| format | Article |
| id | doaj-art-1d3f02080a634d8eb5094f6243461835 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2015-12-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-1d3f02080a634d8eb5094f62434618352025-02-01T09:58:02ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-12-0119552953710.18699/VJ15.069403Identification of cytoplasm types in accessions of the family Brassicaceae (Brassicaceae Burnett) with DNA markersE. A. Domblides0A. S. Domblides1T. V. Zayachkovskaya2L. L. Bondareva3All-Russian Research Institute of Vegetable Breeding and Seed Production, Moscow oblast, Odintsovo region, VNIISSOK, RussiaAll-Russian Research Institute of Vegetable Breeding and Seed Production, Moscow oblast, Odintsovo region, VNIISSOK, RussiaAll-Russian Research Institute of Vegetable Breeding and Seed Production, Moscow oblast, Odintsovo region, VNIISSOK, RussiaAll-Russian Research Institute of Vegetable Breeding and Seed Production, Moscow oblast, Odintsovo region, VNIISSOK, RussiaIdentification of cytoplasm types with the use of molecular markers enables to simplify the parent line pair selection for hybrid development based on CMS. Forty accessions of the family Brassicaceae (Brassicaceae Burnett) were analyzed with 22 pairs of PCR primers taken from previous research. Seven types of cytoplasm (Ogura, Ogu-NWSUAF, nap, pol, cam, rad, ole) were observed among breeding accessions using standard and multiplex PCR technique. The sequence of PCR products that differentiated different types of sterile cytoplasm confirmed the presence of three genes, orf138, orf222, and orf224, in mitochondrial DNA associated with exhibition of CMS. The accession of Nappa cabbage (Brassica rapa Pekinensis Group) had two genes, orf138 and orf222, which corresponded to cytoplasm Ogu-NWSUAF. All accessions carrying Ogura cytoplasm had the 417 bp fragment, which was 100 % identical to the mitochondrial orf138 gene sequence corres- ponding to Type А (formerly nine sequence Types, from A to I, had been identified). An exception was the accession of white head cabbage Tekila F1 with a new allelic variant of orf138 gene, which combined the 39-bp deletion typical of the Type F orf138 sequence and two nonsynonymous substitutions, A→T and G→A, at positions 95 and 99, respectively.https://vavilov.elpub.ru/jour/article/view/445brassicaceae burnettmitochondrial dna (mtdna)cytoplasmic male sterility (cms)cms oguracms ogu-nwsuaforf138pcr |
| spellingShingle | E. A. Domblides A. S. Domblides T. V. Zayachkovskaya L. L. Bondareva Identification of cytoplasm types in accessions of the family Brassicaceae (Brassicaceae Burnett) with DNA markers Вавиловский журнал генетики и селекции brassicaceae burnett mitochondrial dna (mtdna) cytoplasmic male sterility (cms) cms ogura cms ogu-nwsuaf orf138 pcr |
| title | Identification of cytoplasm types in accessions of the family Brassicaceae (Brassicaceae Burnett) with DNA markers |
| title_full | Identification of cytoplasm types in accessions of the family Brassicaceae (Brassicaceae Burnett) with DNA markers |
| title_fullStr | Identification of cytoplasm types in accessions of the family Brassicaceae (Brassicaceae Burnett) with DNA markers |
| title_full_unstemmed | Identification of cytoplasm types in accessions of the family Brassicaceae (Brassicaceae Burnett) with DNA markers |
| title_short | Identification of cytoplasm types in accessions of the family Brassicaceae (Brassicaceae Burnett) with DNA markers |
| title_sort | identification of cytoplasm types in accessions of the family brassicaceae brassicaceae burnett with dna markers |
| topic | brassicaceae burnett mitochondrial dna (mtdna) cytoplasmic male sterility (cms) cms ogura cms ogu-nwsuaf orf138 pcr |
| url | https://vavilov.elpub.ru/jour/article/view/445 |
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