Deletion of bZIP Transcription Factor <i>PratfA</i> Reveals Specialized Metabolites Potentially Regulating Stress Response in <i>Penicillium raistrickii</i>
Fungal secondary metabolism (SM) is highly correlated with physiological processes that are typically regulated by pleiotropic regulators. In this study, we purposefully altered <i>PratfA</i>, a crucial regulator associated with oxidative stress in <i>Penicillium raistrickii</i&...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/11/1/72 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832588183392485376 |
|---|---|
| author | Anxin Zhang Shu Zhang Xinran Xu Wen-Bing Yin |
| author_facet | Anxin Zhang Shu Zhang Xinran Xu Wen-Bing Yin |
| author_sort | Anxin Zhang |
| collection | DOAJ |
| description | Fungal secondary metabolism (SM) is highly correlated with physiological processes that are typically regulated by pleiotropic regulators. In this study, we purposefully altered <i>PratfA</i>, a crucial regulator associated with oxidative stress in <i>Penicillium raistrickii</i> CGMCC 3.1066. After the knockout of <i>PratfA</i>, a novel polyketide (PK) raistrilide A (<b>1</b>) and the known nonribosomal peptide (NRP) tunicoidine (<b>2</b>) subsequently disappeared. Notably, compound <b>1</b> is a rare octaketone derivative and contains two unsubstituted <i>cis</i>-double bonds, demonstrating its unique biosynthetic mechanism. The knockout of <i>PratfA</i> resulted in the disappearance of <b>1</b>–<b>2</b> and greatly increased the susceptibility of Δ<i>PratfA</i> mutant strain to oxidative stress, rendering it nearly impossible to survive in such environments. At present, the <i>OE⸬PratfA</i> strain showed no phenotypic or oxidative stress sensitivity differences compared to the wild-type strain. Our findings highlight that the oxidative-stress-related transcription factor (TF) <i>PratfA</i> influences SM pathways in <i>P</i>. <i>raistrickii</i>. The manipulation of regulatory factors can guide the discovery of novel natural products (NPs). |
| format | Article |
| id | doaj-art-1d327308a70e4f16895e405e991b6227 |
| institution | Kabale University |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-1d327308a70e4f16895e405e991b62272025-01-24T13:37:26ZengMDPI AGJournal of Fungi2309-608X2025-01-011117210.3390/jof11010072Deletion of bZIP Transcription Factor <i>PratfA</i> Reveals Specialized Metabolites Potentially Regulating Stress Response in <i>Penicillium raistrickii</i>Anxin Zhang0Shu Zhang1Xinran Xu2Wen-Bing Yin3State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaFungal secondary metabolism (SM) is highly correlated with physiological processes that are typically regulated by pleiotropic regulators. In this study, we purposefully altered <i>PratfA</i>, a crucial regulator associated with oxidative stress in <i>Penicillium raistrickii</i> CGMCC 3.1066. After the knockout of <i>PratfA</i>, a novel polyketide (PK) raistrilide A (<b>1</b>) and the known nonribosomal peptide (NRP) tunicoidine (<b>2</b>) subsequently disappeared. Notably, compound <b>1</b> is a rare octaketone derivative and contains two unsubstituted <i>cis</i>-double bonds, demonstrating its unique biosynthetic mechanism. The knockout of <i>PratfA</i> resulted in the disappearance of <b>1</b>–<b>2</b> and greatly increased the susceptibility of Δ<i>PratfA</i> mutant strain to oxidative stress, rendering it nearly impossible to survive in such environments. At present, the <i>OE⸬PratfA</i> strain showed no phenotypic or oxidative stress sensitivity differences compared to the wild-type strain. Our findings highlight that the oxidative-stress-related transcription factor (TF) <i>PratfA</i> influences SM pathways in <i>P</i>. <i>raistrickii</i>. The manipulation of regulatory factors can guide the discovery of novel natural products (NPs).https://www.mdpi.com/2309-608X/11/1/72<i>Penicillium raistrickii</i><i>PratfA</i>raistrilide A (<b>1</b>)tunicoidine F (<b>2</b>)oxidative stress |
| spellingShingle | Anxin Zhang Shu Zhang Xinran Xu Wen-Bing Yin Deletion of bZIP Transcription Factor <i>PratfA</i> Reveals Specialized Metabolites Potentially Regulating Stress Response in <i>Penicillium raistrickii</i> Journal of Fungi <i>Penicillium raistrickii</i> <i>PratfA</i> raistrilide A (<b>1</b>) tunicoidine F (<b>2</b>) oxidative stress |
| title | Deletion of bZIP Transcription Factor <i>PratfA</i> Reveals Specialized Metabolites Potentially Regulating Stress Response in <i>Penicillium raistrickii</i> |
| title_full | Deletion of bZIP Transcription Factor <i>PratfA</i> Reveals Specialized Metabolites Potentially Regulating Stress Response in <i>Penicillium raistrickii</i> |
| title_fullStr | Deletion of bZIP Transcription Factor <i>PratfA</i> Reveals Specialized Metabolites Potentially Regulating Stress Response in <i>Penicillium raistrickii</i> |
| title_full_unstemmed | Deletion of bZIP Transcription Factor <i>PratfA</i> Reveals Specialized Metabolites Potentially Regulating Stress Response in <i>Penicillium raistrickii</i> |
| title_short | Deletion of bZIP Transcription Factor <i>PratfA</i> Reveals Specialized Metabolites Potentially Regulating Stress Response in <i>Penicillium raistrickii</i> |
| title_sort | deletion of bzip transcription factor i pratfa i reveals specialized metabolites potentially regulating stress response in i penicillium raistrickii i |
| topic | <i>Penicillium raistrickii</i> <i>PratfA</i> raistrilide A (<b>1</b>) tunicoidine F (<b>2</b>) oxidative stress |
| url | https://www.mdpi.com/2309-608X/11/1/72 |
| work_keys_str_mv | AT anxinzhang deletionofbziptranscriptionfactoripratfairevealsspecializedmetabolitespotentiallyregulatingstressresponseinipenicilliumraistrickiii AT shuzhang deletionofbziptranscriptionfactoripratfairevealsspecializedmetabolitespotentiallyregulatingstressresponseinipenicilliumraistrickiii AT xinranxu deletionofbziptranscriptionfactoripratfairevealsspecializedmetabolitespotentiallyregulatingstressresponseinipenicilliumraistrickiii AT wenbingyin deletionofbziptranscriptionfactoripratfairevealsspecializedmetabolitespotentiallyregulatingstressresponseinipenicilliumraistrickiii |