Knockdown of Zebrafish Blood Vessel Epicardial Substance Results in Incomplete Retinal Lamination
Cell polarity during eye development determines the normal retinal lamination and differentiation of photoreceptor cells in the retina. In vertebrates, blood vessel epicardial substance (Bves) is known to play an important role in the formation and maintenance of the tight junctions essential for ep...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/803718 |
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| author | Yu-Ching Wu Ruei-Feng Chen Chia-Yang Liu Fung Rong Hu Chang-Jen Huang I-Jong Wang |
| author_facet | Yu-Ching Wu Ruei-Feng Chen Chia-Yang Liu Fung Rong Hu Chang-Jen Huang I-Jong Wang |
| author_sort | Yu-Ching Wu |
| collection | DOAJ |
| description | Cell polarity during eye development determines the normal retinal lamination and differentiation of photoreceptor cells in the retina. In vertebrates, blood vessel epicardial substance (Bves) is known to play an important role in the formation and maintenance of the tight junctions essential for epithelial cell polarity. In the current study, we generated a transgenic zebrafish Bves (zbves) promoter-EGFP zebrafish line to investigate the expression pattern of Bves in the retina and to study the role of zbves in retinal lamination. Immunostaining with different specific antibodies from retinal cells and transmission electron microscopy were used to identify the morphological defects in normal and Bves knockdown zebrafish. In normal zebrafish, Bves is located at the apical junctions of embryonic retinal neuroepithelia during retinogenesis; later, it is strongly expressed around inner plexiform layer (IPL) and retinal pigment epithelium (RPE). In contrast, a loss of normal retinal lamination and cellular polarity was found with undifferentiated photoreceptor cells in Bves knockdown zebrafish. Herein, our results indicated that disruption of Bves will result in a loss of normal retinal lamination. |
| format | Article |
| id | doaj-art-1d26fed27ef74d9fad0d7994e50eff1f |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-1d26fed27ef74d9fad0d7994e50eff1f2025-02-03T01:30:17ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/803718803718Knockdown of Zebrafish Blood Vessel Epicardial Substance Results in Incomplete Retinal LaminationYu-Ching Wu0Ruei-Feng Chen1Chia-Yang Liu2Fung Rong Hu3Chang-Jen Huang4I-Jong Wang5Department of Life Science, College of Life Science, National Taiwan University, Taipei 10617, TaiwanDepartment of Life Science, College of Life Science, National Taiwan University, Taipei 10617, TaiwanDepartment of Ophthalmology, Graduate Program of Neuroscience, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USADepartment of Ophthalmology, National Taiwan University Hospital, Taipei 10002, TaiwanInstitute of Biological Chemistry, Academia Sinica, Taipei 11529, TaiwanDepartment of Ophthalmology, National Taiwan University Hospital, Taipei 10002, TaiwanCell polarity during eye development determines the normal retinal lamination and differentiation of photoreceptor cells in the retina. In vertebrates, blood vessel epicardial substance (Bves) is known to play an important role in the formation and maintenance of the tight junctions essential for epithelial cell polarity. In the current study, we generated a transgenic zebrafish Bves (zbves) promoter-EGFP zebrafish line to investigate the expression pattern of Bves in the retina and to study the role of zbves in retinal lamination. Immunostaining with different specific antibodies from retinal cells and transmission electron microscopy were used to identify the morphological defects in normal and Bves knockdown zebrafish. In normal zebrafish, Bves is located at the apical junctions of embryonic retinal neuroepithelia during retinogenesis; later, it is strongly expressed around inner plexiform layer (IPL) and retinal pigment epithelium (RPE). In contrast, a loss of normal retinal lamination and cellular polarity was found with undifferentiated photoreceptor cells in Bves knockdown zebrafish. Herein, our results indicated that disruption of Bves will result in a loss of normal retinal lamination.http://dx.doi.org/10.1155/2014/803718 |
| spellingShingle | Yu-Ching Wu Ruei-Feng Chen Chia-Yang Liu Fung Rong Hu Chang-Jen Huang I-Jong Wang Knockdown of Zebrafish Blood Vessel Epicardial Substance Results in Incomplete Retinal Lamination The Scientific World Journal |
| title | Knockdown of Zebrafish Blood Vessel Epicardial Substance Results in Incomplete Retinal Lamination |
| title_full | Knockdown of Zebrafish Blood Vessel Epicardial Substance Results in Incomplete Retinal Lamination |
| title_fullStr | Knockdown of Zebrafish Blood Vessel Epicardial Substance Results in Incomplete Retinal Lamination |
| title_full_unstemmed | Knockdown of Zebrafish Blood Vessel Epicardial Substance Results in Incomplete Retinal Lamination |
| title_short | Knockdown of Zebrafish Blood Vessel Epicardial Substance Results in Incomplete Retinal Lamination |
| title_sort | knockdown of zebrafish blood vessel epicardial substance results in incomplete retinal lamination |
| url | http://dx.doi.org/10.1155/2014/803718 |
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