Genetic Transformation of Common Bean (Phaseolus vulgaris L.) with the Gus Color Marker, the Bar Herbicide Resistance, and the Barley (Hordeum vulgare) HVA1 Drought Tolerance Genes
Five common bean (Phaseolus vulgaris L.) varieties including “Condor,” “Matterhorn,” “Sedona,” “Olathe,” and “Montcalm” were genetically transformed via the Biolistic bombardment of the apical shoot meristem primordium. Transgenes included gus color marker which visually confirmed transgenic events,...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Agronomy |
| Online Access: | http://dx.doi.org/10.1155/2012/198960 |
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| author | Kingdom Kwapata Thang Nguyen Mariam Sticklen |
| author_facet | Kingdom Kwapata Thang Nguyen Mariam Sticklen |
| author_sort | Kingdom Kwapata |
| collection | DOAJ |
| description | Five common bean (Phaseolus vulgaris L.) varieties including “Condor,” “Matterhorn,” “Sedona,” “Olathe,” and “Montcalm” were genetically transformed via the Biolistic bombardment of the apical shoot meristem primordium. Transgenes included gus color marker which visually confirmed transgenic events, the bar herbicide resistance selectable marker used for in vitro selection of transgenic cultures and which confirmed Liberty herbicide resistant plants, and the barley (Hordeum vulgare) late embryogenesis abundant protein (HVA1) which conferred drought tolerance with a corresponding increase in root length of transgenic plants. Research presented here might assist in production of better P. vulgaris germplasm. |
| format | Article |
| id | doaj-art-1d125ffd586c41d7b1522eaae35186a0 |
| institution | Kabale University |
| issn | 1687-8159 1687-8167 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Agronomy |
| spelling | doaj-art-1d125ffd586c41d7b1522eaae35186a02025-02-03T01:27:51ZengWileyInternational Journal of Agronomy1687-81591687-81672012-01-01201210.1155/2012/198960198960Genetic Transformation of Common Bean (Phaseolus vulgaris L.) with the Gus Color Marker, the Bar Herbicide Resistance, and the Barley (Hordeum vulgare) HVA1 Drought Tolerance GenesKingdom Kwapata0Thang Nguyen1Mariam Sticklen2Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI 48824, USADepartment of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI 48824, USADepartment of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI 48824, USAFive common bean (Phaseolus vulgaris L.) varieties including “Condor,” “Matterhorn,” “Sedona,” “Olathe,” and “Montcalm” were genetically transformed via the Biolistic bombardment of the apical shoot meristem primordium. Transgenes included gus color marker which visually confirmed transgenic events, the bar herbicide resistance selectable marker used for in vitro selection of transgenic cultures and which confirmed Liberty herbicide resistant plants, and the barley (Hordeum vulgare) late embryogenesis abundant protein (HVA1) which conferred drought tolerance with a corresponding increase in root length of transgenic plants. Research presented here might assist in production of better P. vulgaris germplasm.http://dx.doi.org/10.1155/2012/198960 |
| spellingShingle | Kingdom Kwapata Thang Nguyen Mariam Sticklen Genetic Transformation of Common Bean (Phaseolus vulgaris L.) with the Gus Color Marker, the Bar Herbicide Resistance, and the Barley (Hordeum vulgare) HVA1 Drought Tolerance Genes International Journal of Agronomy |
| title | Genetic Transformation of Common Bean (Phaseolus vulgaris L.) with the Gus Color Marker, the Bar Herbicide Resistance, and the Barley (Hordeum vulgare) HVA1 Drought Tolerance Genes |
| title_full | Genetic Transformation of Common Bean (Phaseolus vulgaris L.) with the Gus Color Marker, the Bar Herbicide Resistance, and the Barley (Hordeum vulgare) HVA1 Drought Tolerance Genes |
| title_fullStr | Genetic Transformation of Common Bean (Phaseolus vulgaris L.) with the Gus Color Marker, the Bar Herbicide Resistance, and the Barley (Hordeum vulgare) HVA1 Drought Tolerance Genes |
| title_full_unstemmed | Genetic Transformation of Common Bean (Phaseolus vulgaris L.) with the Gus Color Marker, the Bar Herbicide Resistance, and the Barley (Hordeum vulgare) HVA1 Drought Tolerance Genes |
| title_short | Genetic Transformation of Common Bean (Phaseolus vulgaris L.) with the Gus Color Marker, the Bar Herbicide Resistance, and the Barley (Hordeum vulgare) HVA1 Drought Tolerance Genes |
| title_sort | genetic transformation of common bean phaseolus vulgaris l with the gus color marker the bar herbicide resistance and the barley hordeum vulgare hva1 drought tolerance genes |
| url | http://dx.doi.org/10.1155/2012/198960 |
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