ClC-3 Expression and Its Association with Hyperglycemia Induced HT22 Hippocampal Neuronal Cell Apoptosis
Although apoptosis plays an important role in the development of Diabetic Encephalopathy (DE), the underlying molecular mechanisms remain unclear. With respect to this, the present work aims to study the variation in chloride/proton exchanger ClC-3 expression and its association with HT22 hippocampa...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2016/2984380 |
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| author | Feiyan Fan Tao Liu Xin Wang Dongni Ren Hui Liu Pengxing Zhang Zhen Wang Nan Liu Qian Li Yanyang Tu Jianfang Fu |
| author_facet | Feiyan Fan Tao Liu Xin Wang Dongni Ren Hui Liu Pengxing Zhang Zhen Wang Nan Liu Qian Li Yanyang Tu Jianfang Fu |
| author_sort | Feiyan Fan |
| collection | DOAJ |
| description | Although apoptosis plays an important role in the development of Diabetic Encephalopathy (DE), the underlying molecular mechanisms remain unclear. With respect to this, the present work aims to study the variation in chloride/proton exchanger ClC-3 expression and its association with HT22 hippocampal neuronal apoptosis under hyperglycemic condition in vitro. The cells were stimulated with added 0, 5, or 25 mM glucose or mannitol for up to 72 hours before assessing the rate of ClC-3 expression, cell viability, and apoptosis. In a consecutive experiment, cells received chloride channel blocker in addition to glucose. The rate of cellular death/apoptosis and viability was measured using Flow Cytometry and MTT assay, respectively. Changes in ClC-3 expression were assessed using immunofluorescence staining and western blot analysis. The results revealed a significant increase in cellular apoptosis and reduction in viability, associated with increased ClC-3 expression in high glucose group. Osmolarity had no role to play. Addition of chloride channel blocker completely abolished this effect. Thus we conclude that, with its increased expression, ClC-3 plays a major role in hyperglycemia induced hippocampal neuronal apoptosis. To strengthen our understanding of this aforesaid association, we conducted an extensive literature search which is presented in this paper. |
| format | Article |
| id | doaj-art-1d0a949fd1c04e91a04e620f1d51c962 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-1d0a949fd1c04e91a04e620f1d51c9622025-02-03T05:52:09ZengWileyJournal of Diabetes Research2314-67452314-67532016-01-01201610.1155/2016/29843802984380ClC-3 Expression and Its Association with Hyperglycemia Induced HT22 Hippocampal Neuronal Cell ApoptosisFeiyan Fan0Tao Liu1Xin Wang2Dongni Ren3Hui Liu4Pengxing Zhang5Zhen Wang6Nan Liu7Qian Li8Yanyang Tu9Jianfang Fu10Department of Experimental Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi’an 710038, ChinaDepartment of Dermatology, Tangdu Hospital, The Fourth Military Medical University, Xi’an 710038, ChinaDepartment of Neurosurgery, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02115, USADepartment of Experimental Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi’an 710038, ChinaDepartment of Experimental Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi’an 710038, ChinaDepartment of Experimental Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi’an 710038, ChinaDepartment of Experimental Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi’an 710038, ChinaDepartment of Experimental Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi’an 710038, ChinaDepartment of Experimental Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi’an 710038, ChinaDepartment of Experimental Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi’an 710038, ChinaDepartment of Endocrinology, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, ChinaAlthough apoptosis plays an important role in the development of Diabetic Encephalopathy (DE), the underlying molecular mechanisms remain unclear. With respect to this, the present work aims to study the variation in chloride/proton exchanger ClC-3 expression and its association with HT22 hippocampal neuronal apoptosis under hyperglycemic condition in vitro. The cells were stimulated with added 0, 5, or 25 mM glucose or mannitol for up to 72 hours before assessing the rate of ClC-3 expression, cell viability, and apoptosis. In a consecutive experiment, cells received chloride channel blocker in addition to glucose. The rate of cellular death/apoptosis and viability was measured using Flow Cytometry and MTT assay, respectively. Changes in ClC-3 expression were assessed using immunofluorescence staining and western blot analysis. The results revealed a significant increase in cellular apoptosis and reduction in viability, associated with increased ClC-3 expression in high glucose group. Osmolarity had no role to play. Addition of chloride channel blocker completely abolished this effect. Thus we conclude that, with its increased expression, ClC-3 plays a major role in hyperglycemia induced hippocampal neuronal apoptosis. To strengthen our understanding of this aforesaid association, we conducted an extensive literature search which is presented in this paper.http://dx.doi.org/10.1155/2016/2984380 |
| spellingShingle | Feiyan Fan Tao Liu Xin Wang Dongni Ren Hui Liu Pengxing Zhang Zhen Wang Nan Liu Qian Li Yanyang Tu Jianfang Fu ClC-3 Expression and Its Association with Hyperglycemia Induced HT22 Hippocampal Neuronal Cell Apoptosis Journal of Diabetes Research |
| title | ClC-3 Expression and Its Association with Hyperglycemia Induced HT22 Hippocampal Neuronal Cell Apoptosis |
| title_full | ClC-3 Expression and Its Association with Hyperglycemia Induced HT22 Hippocampal Neuronal Cell Apoptosis |
| title_fullStr | ClC-3 Expression and Its Association with Hyperglycemia Induced HT22 Hippocampal Neuronal Cell Apoptosis |
| title_full_unstemmed | ClC-3 Expression and Its Association with Hyperglycemia Induced HT22 Hippocampal Neuronal Cell Apoptosis |
| title_short | ClC-3 Expression and Its Association with Hyperglycemia Induced HT22 Hippocampal Neuronal Cell Apoptosis |
| title_sort | clc 3 expression and its association with hyperglycemia induced ht22 hippocampal neuronal cell apoptosis |
| url | http://dx.doi.org/10.1155/2016/2984380 |
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