PPAR-α regulates metabolic remodelling and participates in myocardial fibrosis in patients with atrial fibrillation of rheumatic heart disease
Introduction This study will explore the correlation of peroxisome proliferator activated receptor-α (PPAR-α) regulation of metabolic remodelling in the myocardial fibrosis of atrial fibrillation (AF) in rheumatic heart disease. Material and methods The left atrial appendage tissues were evaluated...
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| Language: | English |
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Termedia Publishing House
2024-07-01
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| Series: | Archives of Medical Science |
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| Online Access: | https://www.archivesofmedicalscience.com/PPAR-regulates-metabolic-remodelling-and-nparticipates-in-myocardial-fibrosis-in,181134,0,2.html |
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| _version_ | 1832584845953335296 |
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| author | Xiaoying Hu Daisong Jiang Zheng Zhang Zhenmei An |
| author_facet | Xiaoying Hu Daisong Jiang Zheng Zhang Zhenmei An |
| author_sort | Xiaoying Hu |
| collection | DOAJ |
| description | Introduction
This study will explore the correlation of peroxisome proliferator
activated receptor-α (PPAR-α) regulation of metabolic remodelling in
the myocardial fibrosis of atrial fibrillation (AF) in rheumatic heart disease.
Material and methods
The left atrial appendage tissues were evaluated
by Masson staining for fibrosis degree, and Western Blot was used to detect
the expression of proteins related to glucose metabolism disorder, lipid metabolism
abnormality, and mitochondrial dysfunction. The myocardial fibroblasts
were established by stimulation with ANG II, and the PPAR-α agonist
GW7647 was administered. The changes of phenotype transformation of
myocardial fibroblasts were detected by cellular immunofluorescence, the
secretion level of supernatant collagen was detected by ELISA. Finally, the
correlation between PPAR-α protein expression and myocardial fibrosis was
analysed and a conclusion was drawn.
Results
Masson staining showed that the degree of myocardial fibrosis in
patients with AF was significantly increased; WB analysis showed that there
were statistically significant differences in protein expression related to glucose
metabolism disorder, lipid metabolism abnormality, and mitochondrial
dysfunction. There was a correlation between PPAR-α protein expression
and myocardial fibrosis ( r = –0.5322, p < 0.0001). After stimulation with
PPAR-α agonist GW7647, the phenotypic differentiation of myocardial fibro-
blasts into myofibroblasts was inhibited. The protein expression related
to mitochondrial dysfunction was statistically different.
Conclusions
This study found that there is a negative correlation between
the expression of PPAR-α protein and myocardial fibrosis in rheumatic heart
disease AF, which plays a protective role. PPAR-α may participate in the
pathogenesis of myocardial fibrosis in rheumatic heart disease AF by regulating
glucose metabolism, lipid metabolism, and mitochondrial function. |
| format | Article |
| id | doaj-art-1d055be5d0ab4c608081fa5dfd2a351f |
| institution | Kabale University |
| issn | 1734-1922 1896-9151 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj-art-1d055be5d0ab4c608081fa5dfd2a351f2025-01-27T10:44:30ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512024-07-012051461147110.5114/aoms/181134181134PPAR-α regulates metabolic remodelling and participates in myocardial fibrosis in patients with atrial fibrillation of rheumatic heart diseaseXiaoying Hu0Daisong Jiang1Zheng Zhang2Zhenmei An3https://orcid.org/0000-0001-5952-3701Department of Laboratory Medicine, Zigong Fourth People’s Hospital, Zigong, ChinaDepartment of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, ChinaIntroduction This study will explore the correlation of peroxisome proliferator activated receptor-α (PPAR-α) regulation of metabolic remodelling in the myocardial fibrosis of atrial fibrillation (AF) in rheumatic heart disease. Material and methods The left atrial appendage tissues were evaluated by Masson staining for fibrosis degree, and Western Blot was used to detect the expression of proteins related to glucose metabolism disorder, lipid metabolism abnormality, and mitochondrial dysfunction. The myocardial fibroblasts were established by stimulation with ANG II, and the PPAR-α agonist GW7647 was administered. The changes of phenotype transformation of myocardial fibroblasts were detected by cellular immunofluorescence, the secretion level of supernatant collagen was detected by ELISA. Finally, the correlation between PPAR-α protein expression and myocardial fibrosis was analysed and a conclusion was drawn. Results Masson staining showed that the degree of myocardial fibrosis in patients with AF was significantly increased; WB analysis showed that there were statistically significant differences in protein expression related to glucose metabolism disorder, lipid metabolism abnormality, and mitochondrial dysfunction. There was a correlation between PPAR-α protein expression and myocardial fibrosis ( r = –0.5322, p < 0.0001). After stimulation with PPAR-α agonist GW7647, the phenotypic differentiation of myocardial fibro- blasts into myofibroblasts was inhibited. The protein expression related to mitochondrial dysfunction was statistically different. Conclusions This study found that there is a negative correlation between the expression of PPAR-α protein and myocardial fibrosis in rheumatic heart disease AF, which plays a protective role. PPAR-α may participate in the pathogenesis of myocardial fibrosis in rheumatic heart disease AF by regulating glucose metabolism, lipid metabolism, and mitochondrial function.https://www.archivesofmedicalscience.com/PPAR-regulates-metabolic-remodelling-and-nparticipates-in-myocardial-fibrosis-in,181134,0,2.htmlperoxisome proliferator activated receptor-αrheumatic heartdiseaseatrial fibrillationmetabolic remodellingmyocardial fibrosiscorrelation study |
| spellingShingle | Xiaoying Hu Daisong Jiang Zheng Zhang Zhenmei An PPAR-α regulates metabolic remodelling and participates in myocardial fibrosis in patients with atrial fibrillation of rheumatic heart disease Archives of Medical Science peroxisome proliferator activated receptor-α rheumatic heart disease atrial fibrillation metabolic remodelling myocardial fibrosis correlation study |
| title | PPAR-α regulates metabolic remodelling and
participates in myocardial fibrosis in patients with
atrial fibrillation of rheumatic heart disease |
| title_full | PPAR-α regulates metabolic remodelling and
participates in myocardial fibrosis in patients with
atrial fibrillation of rheumatic heart disease |
| title_fullStr | PPAR-α regulates metabolic remodelling and
participates in myocardial fibrosis in patients with
atrial fibrillation of rheumatic heart disease |
| title_full_unstemmed | PPAR-α regulates metabolic remodelling and
participates in myocardial fibrosis in patients with
atrial fibrillation of rheumatic heart disease |
| title_short | PPAR-α regulates metabolic remodelling and
participates in myocardial fibrosis in patients with
atrial fibrillation of rheumatic heart disease |
| title_sort | ppar α regulates metabolic remodelling and participates in myocardial fibrosis in patients with atrial fibrillation of rheumatic heart disease |
| topic | peroxisome proliferator activated receptor-α rheumatic heart disease atrial fibrillation metabolic remodelling myocardial fibrosis correlation study |
| url | https://www.archivesofmedicalscience.com/PPAR-regulates-metabolic-remodelling-and-nparticipates-in-myocardial-fibrosis-in,181134,0,2.html |
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