High Serum Advanced Glycation End Products Are Associated with Decreased Insulin Secretion in Patients with Type 2 Diabetes: A Brief Report
Objective. Advanced glycation end products (AGEs) are important in the pathophysiology of type 2 diabetes mellitus (T2DM). They directly cause insulin secretory defects in animal and cell culture models and may promote insulin resistance in nondiabetic subjects. We have developed a highly sensitive...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2017/5139750 |
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| author | Tsuyoshi Okura Etsuko Ueta Risa Nakamura Yohei Fujioka Keisuke Sumi Kazuhisa Matsumoto Kyoko Shoji Kazuhiko Matsuzawa Shoichiro Izawa Yuri Nomi Hitomi Mihara Yuzuru Otsuka Masahiko Kato Shin-ichi Taniguchi Kazuhiro Yamamoto |
| author_facet | Tsuyoshi Okura Etsuko Ueta Risa Nakamura Yohei Fujioka Keisuke Sumi Kazuhisa Matsumoto Kyoko Shoji Kazuhiko Matsuzawa Shoichiro Izawa Yuri Nomi Hitomi Mihara Yuzuru Otsuka Masahiko Kato Shin-ichi Taniguchi Kazuhiro Yamamoto |
| author_sort | Tsuyoshi Okura |
| collection | DOAJ |
| description | Objective. Advanced glycation end products (AGEs) are important in the pathophysiology of type 2 diabetes mellitus (T2DM). They directly cause insulin secretory defects in animal and cell culture models and may promote insulin resistance in nondiabetic subjects. We have developed a highly sensitive liquid chromatography-tandem mass spectrometry method for measuring AGEs in human serum. Here, we use this method to investigate the relationship between AGEs and insulin secretion and resistance in patients with T2DM. Methods. Our study involved 15 participants with T2DM not on medication and 20 nondiabetic healthy participants. We measured the AGE carboxyethyllysine (CEL), carboxymethyllysine (CML), and methyl-glyoxal-hydro-imidazolone (MG-H1). Plasma glucose and insulin were measured in these participants during a meal tolerance test, and the glucose disposal rate was measured during a euglycemic-hyperinsulinemic clamp. Results. CML and CEL levels were significantly higher in T2DM than non-DM participants. CML showed a significant negative correlation with insulin secretion, HOMA-%B, and a significant positive correlation with the insulin sensitivity index in T2DM participants. There was no correlation between any of the AGEs measured and glucose disposal rate. Conclusions. These results suggest that AGE might play a role in the development or prediction of insulin secretory defects in type 2 diabetes. |
| format | Article |
| id | doaj-art-1cfd3c4fc4bb49a4806cd01f6eb18921 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-1cfd3c4fc4bb49a4806cd01f6eb189212025-02-03T05:48:19ZengWileyJournal of Diabetes Research2314-67452314-67532017-01-01201710.1155/2017/51397505139750High Serum Advanced Glycation End Products Are Associated with Decreased Insulin Secretion in Patients with Type 2 Diabetes: A Brief ReportTsuyoshi Okura0Etsuko Ueta1Risa Nakamura2Yohei Fujioka3Keisuke Sumi4Kazuhisa Matsumoto5Kyoko Shoji6Kazuhiko Matsuzawa7Shoichiro Izawa8Yuri Nomi9Hitomi Mihara10Yuzuru Otsuka11Masahiko Kato12Shin-ichi Taniguchi13Kazuhiro Yamamoto14Division of Cardiovascular Medicine, Endocrinology and Metabolism, Department of Molecular Medicine and Therapeutics, Tottori University Faculty of Medicine, Yonago, Tottori, JapanSchool of Health Science, Tottori University Faculty of Medicine, Yonago, Tottori, JapanDivision of Cardiovascular Medicine, Endocrinology and Metabolism, Department of Molecular Medicine and Therapeutics, Tottori University Faculty of Medicine, Yonago, Tottori, JapanDivision of Cardiovascular Medicine, Endocrinology and Metabolism, Department of Molecular Medicine and Therapeutics, Tottori University Faculty of Medicine, Yonago, Tottori, JapanDivision of Cardiovascular Medicine, Endocrinology and Metabolism, Department of Molecular Medicine and Therapeutics, Tottori University Faculty of Medicine, Yonago, Tottori, JapanDivision of Cardiovascular Medicine, Endocrinology and Metabolism, Department of Molecular Medicine and Therapeutics, Tottori University Faculty of Medicine, Yonago, Tottori, JapanDivision of Cardiovascular Medicine, Endocrinology and Metabolism, Department of Molecular Medicine and Therapeutics, Tottori University Faculty of Medicine, Yonago, Tottori, JapanDepartment of Regional Medicine, Tottori University Faculty of Medicine, Yonago, Tottori, JapanDivision of Cardiovascular Medicine, Endocrinology and Metabolism, Department of Molecular Medicine and Therapeutics, Tottori University Faculty of Medicine, Yonago, Tottori, JapanFaculty of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata, JapanDepartment of Food and Nutrition, Toita Women’s College, Tokyo, JapanDepartment of Food and Nutrition, Toita Women’s College, Tokyo, JapanDivision of Cardiovascular Medicine, Endocrinology and Metabolism, Department of Molecular Medicine and Therapeutics, Tottori University Faculty of Medicine, Yonago, Tottori, JapanDepartment of Regional Medicine, Tottori University Faculty of Medicine, Yonago, Tottori, JapanDivision of Cardiovascular Medicine, Endocrinology and Metabolism, Department of Molecular Medicine and Therapeutics, Tottori University Faculty of Medicine, Yonago, Tottori, JapanObjective. Advanced glycation end products (AGEs) are important in the pathophysiology of type 2 diabetes mellitus (T2DM). They directly cause insulin secretory defects in animal and cell culture models and may promote insulin resistance in nondiabetic subjects. We have developed a highly sensitive liquid chromatography-tandem mass spectrometry method for measuring AGEs in human serum. Here, we use this method to investigate the relationship between AGEs and insulin secretion and resistance in patients with T2DM. Methods. Our study involved 15 participants with T2DM not on medication and 20 nondiabetic healthy participants. We measured the AGE carboxyethyllysine (CEL), carboxymethyllysine (CML), and methyl-glyoxal-hydro-imidazolone (MG-H1). Plasma glucose and insulin were measured in these participants during a meal tolerance test, and the glucose disposal rate was measured during a euglycemic-hyperinsulinemic clamp. Results. CML and CEL levels were significantly higher in T2DM than non-DM participants. CML showed a significant negative correlation with insulin secretion, HOMA-%B, and a significant positive correlation with the insulin sensitivity index in T2DM participants. There was no correlation between any of the AGEs measured and glucose disposal rate. Conclusions. These results suggest that AGE might play a role in the development or prediction of insulin secretory defects in type 2 diabetes.http://dx.doi.org/10.1155/2017/5139750 |
| spellingShingle | Tsuyoshi Okura Etsuko Ueta Risa Nakamura Yohei Fujioka Keisuke Sumi Kazuhisa Matsumoto Kyoko Shoji Kazuhiko Matsuzawa Shoichiro Izawa Yuri Nomi Hitomi Mihara Yuzuru Otsuka Masahiko Kato Shin-ichi Taniguchi Kazuhiro Yamamoto High Serum Advanced Glycation End Products Are Associated with Decreased Insulin Secretion in Patients with Type 2 Diabetes: A Brief Report Journal of Diabetes Research |
| title | High Serum Advanced Glycation End Products Are Associated with Decreased Insulin Secretion in Patients with Type 2 Diabetes: A Brief Report |
| title_full | High Serum Advanced Glycation End Products Are Associated with Decreased Insulin Secretion in Patients with Type 2 Diabetes: A Brief Report |
| title_fullStr | High Serum Advanced Glycation End Products Are Associated with Decreased Insulin Secretion in Patients with Type 2 Diabetes: A Brief Report |
| title_full_unstemmed | High Serum Advanced Glycation End Products Are Associated with Decreased Insulin Secretion in Patients with Type 2 Diabetes: A Brief Report |
| title_short | High Serum Advanced Glycation End Products Are Associated with Decreased Insulin Secretion in Patients with Type 2 Diabetes: A Brief Report |
| title_sort | high serum advanced glycation end products are associated with decreased insulin secretion in patients with type 2 diabetes a brief report |
| url | http://dx.doi.org/10.1155/2017/5139750 |
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