The Effects of Pitavastatin on Nuclear Factor-Kappa B and ICAM-1 in Human Saphenous Vein Graft Endothelial Culture
Objective. To study pitavastatin’s effects on nuclear factor-kappa B (NF-κB ) and adhesion molecules in human saphenous vein graft endothelial culture indicating its pleotropic properties. Materials and Method. Low-dose (0.1 μM/L) and high-dose (1μM/L) pitavastatin calcium were administered as a fro...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Cardiovascular Therapeutics |
| Online Access: | http://dx.doi.org/10.1155/2019/2549432 |
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| _version_ | 1832548036708925440 |
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| author | Bulent Demir Burak Onal Sibel Ozyazgan Esra Demir Vedat Bakuy Ahmet Gokhan Akkan |
| author_facet | Bulent Demir Burak Onal Sibel Ozyazgan Esra Demir Vedat Bakuy Ahmet Gokhan Akkan |
| author_sort | Bulent Demir |
| collection | DOAJ |
| description | Objective. To study pitavastatin’s effects on nuclear factor-kappa B (NF-κB ) and adhesion molecules in human saphenous vein graft endothelial culture indicating its pleotropic properties. Materials and Method. Low-dose (0.1 μM/L) and high-dose (1μM/L) pitavastatin calcium were administered as a frontline therapy in human saphenous endothelial cell culture, followed by induction of inflammation by TNF-α and determination of mRNA level alterations of ICAM-1 and NF-κB genes of endothelial cells using the qRT-PCR method. Additionally, immunofluorescence method was used to show the expression of NF-κB and ICAM-1. Finally, LDH levels were determined by the ELISA method to quantify cytotoxicity. Results. ICAM-1 mRNA expression in the low-dose pitavastatin+TNF-α group was significantly higher than that in the TNF-α group and significantly lower than that in the high-dose pitavastatin+TNF-α group (for all comparisons, P = 0.001). The low-dose pitavastatin+TNF-α group had a similar NF-κB mRNA expression with TNF-α and high-dose pitavastatin+TNF-α groups. Conclusion. Pitavastatin increases ICAM-1 mRNA expression in saphenous vein endothelial cells. Furthermore, the effect of pitavastatin on adhesion molecules appears independent of NF-κB. Novel studies are needed in this field. |
| format | Article |
| id | doaj-art-1cdcc18ca8b14eb8b0e46531cdef680d |
| institution | Kabale University |
| issn | 1755-5914 1755-5922 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cardiovascular Therapeutics |
| spelling | doaj-art-1cdcc18ca8b14eb8b0e46531cdef680d2025-02-03T06:42:18ZengWileyCardiovascular Therapeutics1755-59141755-59222019-01-01201910.1155/2019/25494322549432The Effects of Pitavastatin on Nuclear Factor-Kappa B and ICAM-1 in Human Saphenous Vein Graft Endothelial CultureBulent Demir0Burak Onal1Sibel Ozyazgan2Esra Demir3Vedat Bakuy4Ahmet Gokhan Akkan5Health Sciences University, Bakırköy Dr. Sadi Konuk Education And Research Hospital, Department Of Cardiology, Istanbul, TurkeyBiruni Univesity Medical Faculty, Department Of Medical Pharmacology, Istanbul, TurkeyIstabul Cerrahpasa University Medical Faculty, Department Of Medical Pharmacology, Istanbul, TurkeyHealth Sciences University, Kanuni Sultan Suleyman Education And Research Hospital, Department Of Internal Medicine, Istanbul, TurkeyIstanbul Baskent University Medical Faculty, Department Of Cardiovascular Surgery, Istanbul, TurkeyIstabul Cerrahpasa University Medical Faculty, Department Of Medical Pharmacology, Istanbul, TurkeyObjective. To study pitavastatin’s effects on nuclear factor-kappa B (NF-κB ) and adhesion molecules in human saphenous vein graft endothelial culture indicating its pleotropic properties. Materials and Method. Low-dose (0.1 μM/L) and high-dose (1μM/L) pitavastatin calcium were administered as a frontline therapy in human saphenous endothelial cell culture, followed by induction of inflammation by TNF-α and determination of mRNA level alterations of ICAM-1 and NF-κB genes of endothelial cells using the qRT-PCR method. Additionally, immunofluorescence method was used to show the expression of NF-κB and ICAM-1. Finally, LDH levels were determined by the ELISA method to quantify cytotoxicity. Results. ICAM-1 mRNA expression in the low-dose pitavastatin+TNF-α group was significantly higher than that in the TNF-α group and significantly lower than that in the high-dose pitavastatin+TNF-α group (for all comparisons, P = 0.001). The low-dose pitavastatin+TNF-α group had a similar NF-κB mRNA expression with TNF-α and high-dose pitavastatin+TNF-α groups. Conclusion. Pitavastatin increases ICAM-1 mRNA expression in saphenous vein endothelial cells. Furthermore, the effect of pitavastatin on adhesion molecules appears independent of NF-κB. Novel studies are needed in this field.http://dx.doi.org/10.1155/2019/2549432 |
| spellingShingle | Bulent Demir Burak Onal Sibel Ozyazgan Esra Demir Vedat Bakuy Ahmet Gokhan Akkan The Effects of Pitavastatin on Nuclear Factor-Kappa B and ICAM-1 in Human Saphenous Vein Graft Endothelial Culture Cardiovascular Therapeutics |
| title | The Effects of Pitavastatin on Nuclear Factor-Kappa B and ICAM-1 in Human Saphenous Vein Graft Endothelial Culture |
| title_full | The Effects of Pitavastatin on Nuclear Factor-Kappa B and ICAM-1 in Human Saphenous Vein Graft Endothelial Culture |
| title_fullStr | The Effects of Pitavastatin on Nuclear Factor-Kappa B and ICAM-1 in Human Saphenous Vein Graft Endothelial Culture |
| title_full_unstemmed | The Effects of Pitavastatin on Nuclear Factor-Kappa B and ICAM-1 in Human Saphenous Vein Graft Endothelial Culture |
| title_short | The Effects of Pitavastatin on Nuclear Factor-Kappa B and ICAM-1 in Human Saphenous Vein Graft Endothelial Culture |
| title_sort | effects of pitavastatin on nuclear factor kappa b and icam 1 in human saphenous vein graft endothelial culture |
| url | http://dx.doi.org/10.1155/2019/2549432 |
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