Urinary Exosomal Long Noncoding RNA TERC as a Noninvasive Diagnostic and Prognostic Biomarker for Bladder Urothelial Carcinoma
Purpose. Bladder cancer is one of the most common urological malignancies worldwide, and approximately 90% of bladder cancer cases are histologically typed as bladder urothelial carcinoma (BLCA). Exosomes are 30 to 200 nm extracellular vesicles that transport microRNAs, long noncoding RNAs (lncRNAs)...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/9038808 |
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| author | Chen Chen Anquan Shang Zujun Sun Yuting Gao Jingjuan Huang Yili Ping Wenjing Chang Chenzheng Gu Junjun Sun Ping Ji Yi Yuan Renquan Lu Dong Li |
| author_facet | Chen Chen Anquan Shang Zujun Sun Yuting Gao Jingjuan Huang Yili Ping Wenjing Chang Chenzheng Gu Junjun Sun Ping Ji Yi Yuan Renquan Lu Dong Li |
| author_sort | Chen Chen |
| collection | DOAJ |
| description | Purpose. Bladder cancer is one of the most common urological malignancies worldwide, and approximately 90% of bladder cancer cases are histologically typed as bladder urothelial carcinoma (BLCA). Exosomes are 30 to 200 nm extracellular vesicles that transport microRNAs, long noncoding RNAs (lncRNAs), mRNAs, circular RNAs, and proteins across tissues and through circulation. Urinary exosomes may contain genetic information from tumor cells. Herein, we explored the clinical significance of urinary exosomal lncRNA telomerase RNA component (TERC) levels to provide an urgently needed diagnostic and prognostic biomarker for BLCA. Materials and Methods. In this study, we used RNA-sequencing of samples from four BLCA patients and three healthy controls to identify that TERC was differentially expressed in urinary exosomes. We then used quantitative PCR in different types of clinical samples to validate the biomarker and analyzed results using receiver operating characteristic curves. Results. We found that TERC was significantly upregulated in urinary exosomes from BLCA patients compared with those from healthy controls (P<0.0001). Urinary exosomal TERC showed higher sensitivity (78.65%) and accuracy (77.78%) than existing indicators including nuclear matrix protein-22 and urine cytometry. Using the cut-off value 4.302, the area under the curve for urinary exosomal TERC was 0.836 (95% confidence interval: 0.768–0.891, P<0.0001). Furthermore, this noninvasive assay could distinguish low-grade and high-grade tumors (P=0.0153). Conclusions. TERC is enriched in urinary exosomes from BLCA patients. Urinary exosomal TERC could become a diagnostic and prognostic biomarker for BLCA that allows clinicians to realize noninvasive detection of BLCA. |
| format | Article |
| id | doaj-art-1cd9fb2e9756414d8cb09ab3e1c8d802 |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-1cd9fb2e9756414d8cb09ab3e1c8d8022025-02-03T05:53:27ZengWileyJournal of Immunology Research2314-71562022-01-01202210.1155/2022/9038808Urinary Exosomal Long Noncoding RNA TERC as a Noninvasive Diagnostic and Prognostic Biomarker for Bladder Urothelial CarcinomaChen Chen0Anquan Shang1Zujun Sun2Yuting Gao3Jingjuan Huang4Yili Ping5Wenjing Chang6Chenzheng Gu7Junjun Sun8Ping Ji9Yi Yuan10Renquan Lu11Dong Li12Department of Laboratory MedicineDepartment of Laboratory MedicineDepartment of Laboratory MedicineDepartment of Laboratory MedicineDepartment of Laboratory MedicineDepartment of Laboratory MedicineDepartment of Laboratory MedicineDepartment of Laboratory MedicineDepartment of Laboratory MedicineDepartment of Laboratory MedicineDepartment of Laboratory MedicineDepartment of Clinical LaboratoryDepartment of Laboratory MedicinePurpose. Bladder cancer is one of the most common urological malignancies worldwide, and approximately 90% of bladder cancer cases are histologically typed as bladder urothelial carcinoma (BLCA). Exosomes are 30 to 200 nm extracellular vesicles that transport microRNAs, long noncoding RNAs (lncRNAs), mRNAs, circular RNAs, and proteins across tissues and through circulation. Urinary exosomes may contain genetic information from tumor cells. Herein, we explored the clinical significance of urinary exosomal lncRNA telomerase RNA component (TERC) levels to provide an urgently needed diagnostic and prognostic biomarker for BLCA. Materials and Methods. In this study, we used RNA-sequencing of samples from four BLCA patients and three healthy controls to identify that TERC was differentially expressed in urinary exosomes. We then used quantitative PCR in different types of clinical samples to validate the biomarker and analyzed results using receiver operating characteristic curves. Results. We found that TERC was significantly upregulated in urinary exosomes from BLCA patients compared with those from healthy controls (P<0.0001). Urinary exosomal TERC showed higher sensitivity (78.65%) and accuracy (77.78%) than existing indicators including nuclear matrix protein-22 and urine cytometry. Using the cut-off value 4.302, the area under the curve for urinary exosomal TERC was 0.836 (95% confidence interval: 0.768–0.891, P<0.0001). Furthermore, this noninvasive assay could distinguish low-grade and high-grade tumors (P=0.0153). Conclusions. TERC is enriched in urinary exosomes from BLCA patients. Urinary exosomal TERC could become a diagnostic and prognostic biomarker for BLCA that allows clinicians to realize noninvasive detection of BLCA.http://dx.doi.org/10.1155/2022/9038808 |
| spellingShingle | Chen Chen Anquan Shang Zujun Sun Yuting Gao Jingjuan Huang Yili Ping Wenjing Chang Chenzheng Gu Junjun Sun Ping Ji Yi Yuan Renquan Lu Dong Li Urinary Exosomal Long Noncoding RNA TERC as a Noninvasive Diagnostic and Prognostic Biomarker for Bladder Urothelial Carcinoma Journal of Immunology Research |
| title | Urinary Exosomal Long Noncoding RNA TERC as a Noninvasive Diagnostic and Prognostic Biomarker for Bladder Urothelial Carcinoma |
| title_full | Urinary Exosomal Long Noncoding RNA TERC as a Noninvasive Diagnostic and Prognostic Biomarker for Bladder Urothelial Carcinoma |
| title_fullStr | Urinary Exosomal Long Noncoding RNA TERC as a Noninvasive Diagnostic and Prognostic Biomarker for Bladder Urothelial Carcinoma |
| title_full_unstemmed | Urinary Exosomal Long Noncoding RNA TERC as a Noninvasive Diagnostic and Prognostic Biomarker for Bladder Urothelial Carcinoma |
| title_short | Urinary Exosomal Long Noncoding RNA TERC as a Noninvasive Diagnostic and Prognostic Biomarker for Bladder Urothelial Carcinoma |
| title_sort | urinary exosomal long noncoding rna terc as a noninvasive diagnostic and prognostic biomarker for bladder urothelial carcinoma |
| url | http://dx.doi.org/10.1155/2022/9038808 |
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