Identification of Key Genes and Pathways Involved in Circulating Tumor Cells in Colorectal Cancer
Background. Characterization of the features associated with circulating tumor cells (CTCs) is one of major interests for predicting clinical outcome of colorectal cancer (CRC) patients. However, the molecular features of CTCs remain largely unclear. Methods. For identification of key genes and path...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2022/9943571 |
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| author | Ruijun Pan Chaoran Yu Yanfei Shao Hiju Hong Jing Sun Zhou Zhang Peiyong Li Minhua Zheng |
| author_facet | Ruijun Pan Chaoran Yu Yanfei Shao Hiju Hong Jing Sun Zhou Zhang Peiyong Li Minhua Zheng |
| author_sort | Ruijun Pan |
| collection | DOAJ |
| description | Background. Characterization of the features associated with circulating tumor cells (CTCs) is one of major interests for predicting clinical outcome of colorectal cancer (CRC) patients. However, the molecular features of CTCs remain largely unclear. Methods. For identification of key genes and pathways, GSE31023, contained CTCs from six metastatic CRC patients and three controls, was retrieved for differentially expressed gene (DEG) analysis. Protein-protein interaction networks of DEGs were constructed. Hub genes from the network were prognostic analyzed, as well as the association with tumor-infiltrating immune cells. Results. 1353 DEGs were identified between the CTC and control groups, with 403 genes upregulated and 950 downregulated. 32 pathways were significantly enriched in KEGG, with ribosome pathway as top. The top 10 hub genes were included, including eukaryotic translation elongation factor 2 (EEF2), ribosomal protein S2 (RPS2), ribosomal protein S5 (RPS5), ribosomal protein L3 (RPL3), ribosomal protein S3 (RPS3), ribosomal protein S14 (RPS14), ribosomal protein SA (RPSA), eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), ribosomal protein S15a (RPS15A), and ribosomal protein L4 (RPL4). The correlation between CD4+ T cells and RPS14 (correlation=−0.5) was the highest in colon cancer while CD8+ T and RPS2 (correlation=−0.53) was the highest in rectal cancer. Conclusion. This study identified potential role of ribosome pathway in CTC, providing further insightful therapeutic targets and biomarkers for CRC. |
| format | Article |
| id | doaj-art-1ca204e6fa7c4ff39752cf2b1a0fe293 |
| institution | Kabale University |
| issn | 2210-7185 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-1ca204e6fa7c4ff39752cf2b1a0fe2932025-02-03T01:11:55ZengWileyAnalytical Cellular Pathology2210-71852022-01-01202210.1155/2022/9943571Identification of Key Genes and Pathways Involved in Circulating Tumor Cells in Colorectal CancerRuijun Pan0Chaoran Yu1Yanfei Shao2Hiju Hong3Jing Sun4Zhou Zhang5Peiyong Li6Minhua Zheng7Department of General SurgeryDepartment of General SurgeryDepartment of General SurgeryDepartment of General SurgeryDepartment of General SurgeryDepartment of General SurgeryDepartment of Nuclear MedicineDepartment of General SurgeryBackground. Characterization of the features associated with circulating tumor cells (CTCs) is one of major interests for predicting clinical outcome of colorectal cancer (CRC) patients. However, the molecular features of CTCs remain largely unclear. Methods. For identification of key genes and pathways, GSE31023, contained CTCs from six metastatic CRC patients and three controls, was retrieved for differentially expressed gene (DEG) analysis. Protein-protein interaction networks of DEGs were constructed. Hub genes from the network were prognostic analyzed, as well as the association with tumor-infiltrating immune cells. Results. 1353 DEGs were identified between the CTC and control groups, with 403 genes upregulated and 950 downregulated. 32 pathways were significantly enriched in KEGG, with ribosome pathway as top. The top 10 hub genes were included, including eukaryotic translation elongation factor 2 (EEF2), ribosomal protein S2 (RPS2), ribosomal protein S5 (RPS5), ribosomal protein L3 (RPL3), ribosomal protein S3 (RPS3), ribosomal protein S14 (RPS14), ribosomal protein SA (RPSA), eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), ribosomal protein S15a (RPS15A), and ribosomal protein L4 (RPL4). The correlation between CD4+ T cells and RPS14 (correlation=−0.5) was the highest in colon cancer while CD8+ T and RPS2 (correlation=−0.53) was the highest in rectal cancer. Conclusion. This study identified potential role of ribosome pathway in CTC, providing further insightful therapeutic targets and biomarkers for CRC.http://dx.doi.org/10.1155/2022/9943571 |
| spellingShingle | Ruijun Pan Chaoran Yu Yanfei Shao Hiju Hong Jing Sun Zhou Zhang Peiyong Li Minhua Zheng Identification of Key Genes and Pathways Involved in Circulating Tumor Cells in Colorectal Cancer Analytical Cellular Pathology |
| title | Identification of Key Genes and Pathways Involved in Circulating Tumor Cells in Colorectal Cancer |
| title_full | Identification of Key Genes and Pathways Involved in Circulating Tumor Cells in Colorectal Cancer |
| title_fullStr | Identification of Key Genes and Pathways Involved in Circulating Tumor Cells in Colorectal Cancer |
| title_full_unstemmed | Identification of Key Genes and Pathways Involved in Circulating Tumor Cells in Colorectal Cancer |
| title_short | Identification of Key Genes and Pathways Involved in Circulating Tumor Cells in Colorectal Cancer |
| title_sort | identification of key genes and pathways involved in circulating tumor cells in colorectal cancer |
| url | http://dx.doi.org/10.1155/2022/9943571 |
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