Intranasal Inoculation of Cationic Crosslinked Carbon Dots‐Adjuvanted Respiratory Syncytial Virus F Subunit Vaccine Elicits Mucosal and Systemic Humoral and Cellular Immunity
ABSTRACT Respiratory syncytial virus (RSV) causes severe acute lower respiratory tract infections, especially in infants and the elderly. Developing an RSV vaccine that promotes a robust mucosal immune response is necessary to successfully prevent viral transmission and the development of severe dis...
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2025-04-01
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| Online Access: | https://doi.org/10.1002/mco2.70146 |
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| author | Hong Lei Aqu Alu Jingyun Yang Cai He Jie Shi Weiqi Hong Dandan Peng Yu Zhang Jian Liu Furong Qin Xiya Huang Chunjun Ye Lijiao Pei Xuemei He Hong Yan Guangwen Lu Xiangrong Song Xiawei Wei Yuquan Wei |
| author_facet | Hong Lei Aqu Alu Jingyun Yang Cai He Jie Shi Weiqi Hong Dandan Peng Yu Zhang Jian Liu Furong Qin Xiya Huang Chunjun Ye Lijiao Pei Xuemei He Hong Yan Guangwen Lu Xiangrong Song Xiawei Wei Yuquan Wei |
| author_sort | Hong Lei |
| collection | DOAJ |
| description | ABSTRACT Respiratory syncytial virus (RSV) causes severe acute lower respiratory tract infections, especially in infants and the elderly. Developing an RSV vaccine that promotes a robust mucosal immune response is necessary to successfully prevent viral transmission and the development of severe disease. We previously reported that crosslinked carbon dots (CCD) may be an excellent adjuvant candidate for intranasal (IN) protein subunit vaccines. Considering the strong immunogenicity of RSV prefused F protein (preF), we prepared an IN RSV vaccine composed of the CCD adjuvant and the preF protein as antigen (CCD/preF) and evaluated the induced antigen‐specific humoral and cellular immunity. We found that IN immunization with the CCD/preF vaccine elicited strong serum IgG responses and mucosal immunity, including secreted IgA antibodies, tissue‐resident memory T (TRM) cells, and antigen‐specific B cells, which lasted for at least 1 year. In addition, a combination of intramuscular and IN immunization with CCD/preF vaccine induced stronger systemic and mucosal immunity. Together, this study proved the high immunogenicity of the CCD/preF vaccines and supported the university of the mucosal CCD adjuvant, supporting further development of the CCD/preF vaccine in larger animal models and clinical studies. |
| format | Article |
| id | doaj-art-1c8650b35efa41c7a1d1af88c0f829e3 |
| institution | OA Journals |
| issn | 2688-2663 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | MedComm |
| spelling | doaj-art-1c8650b35efa41c7a1d1af88c0f829e32025-08-20T02:17:34ZengWileyMedComm2688-26632025-04-0164n/an/a10.1002/mco2.70146Intranasal Inoculation of Cationic Crosslinked Carbon Dots‐Adjuvanted Respiratory Syncytial Virus F Subunit Vaccine Elicits Mucosal and Systemic Humoral and Cellular ImmunityHong Lei0Aqu Alu1Jingyun Yang2Cai He3Jie Shi4Weiqi Hong5Dandan Peng6Yu Zhang7Jian Liu8Furong Qin9Xiya Huang10Chunjun Ye11Lijiao Pei12Xuemei He13Hong Yan14Guangwen Lu15Xiangrong Song16Xiawei Wei17Yuquan Wei18Laboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer Center National Clinical Research Center for Geriatrics West China Hospital Sichuan University Chengdu Sichuan People's Republic of ChinaLaboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer Center National Clinical Research Center for Geriatrics West China Hospital Sichuan University Chengdu Sichuan People's Republic of ChinaLaboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer Center National Clinical Research Center for Geriatrics West China Hospital Sichuan University Chengdu Sichuan People's Republic of ChinaLaboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer Center National Clinical Research Center for Geriatrics West China Hospital Sichuan University Chengdu Sichuan People's Republic of ChinaLaboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer Center National Clinical Research Center for Geriatrics West China Hospital Sichuan University Chengdu Sichuan People's Republic of ChinaLaboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer Center National Clinical Research Center for Geriatrics West China Hospital Sichuan University Chengdu Sichuan People's Republic of ChinaLaboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer Center National Clinical Research Center for Geriatrics West China Hospital Sichuan University Chengdu Sichuan People's Republic of ChinaLaboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer Center National Clinical Research Center for Geriatrics West China Hospital Sichuan University Chengdu Sichuan People's Republic of ChinaLaboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer Center National Clinical Research Center for Geriatrics West China Hospital Sichuan University Chengdu Sichuan People's Republic of ChinaLaboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer Center National Clinical Research Center for Geriatrics West China Hospital Sichuan University Chengdu Sichuan People's Republic of ChinaLaboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer Center National Clinical Research Center for Geriatrics West China Hospital Sichuan University Chengdu Sichuan People's Republic of ChinaLaboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer Center National Clinical Research Center for Geriatrics West China Hospital Sichuan University Chengdu Sichuan People's Republic of ChinaLaboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer Center National Clinical Research Center for Geriatrics West China Hospital Sichuan University Chengdu Sichuan People's Republic of ChinaLaboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer Center National Clinical Research Center for Geriatrics West China Hospital Sichuan University Chengdu Sichuan People's Republic of ChinaLaboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer Center National Clinical Research Center for Geriatrics West China Hospital Sichuan University Chengdu Sichuan People's Republic of ChinaLaboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer Center National Clinical Research Center for Geriatrics West China Hospital Sichuan University Chengdu Sichuan People's Republic of ChinaLaboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer Center National Clinical Research Center for Geriatrics West China Hospital Sichuan University Chengdu Sichuan People's Republic of ChinaLaboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer Center National Clinical Research Center for Geriatrics West China Hospital Sichuan University Chengdu Sichuan People's Republic of ChinaLaboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer Center National Clinical Research Center for Geriatrics West China Hospital Sichuan University Chengdu Sichuan People's Republic of ChinaABSTRACT Respiratory syncytial virus (RSV) causes severe acute lower respiratory tract infections, especially in infants and the elderly. Developing an RSV vaccine that promotes a robust mucosal immune response is necessary to successfully prevent viral transmission and the development of severe disease. We previously reported that crosslinked carbon dots (CCD) may be an excellent adjuvant candidate for intranasal (IN) protein subunit vaccines. Considering the strong immunogenicity of RSV prefused F protein (preF), we prepared an IN RSV vaccine composed of the CCD adjuvant and the preF protein as antigen (CCD/preF) and evaluated the induced antigen‐specific humoral and cellular immunity. We found that IN immunization with the CCD/preF vaccine elicited strong serum IgG responses and mucosal immunity, including secreted IgA antibodies, tissue‐resident memory T (TRM) cells, and antigen‐specific B cells, which lasted for at least 1 year. In addition, a combination of intramuscular and IN immunization with CCD/preF vaccine induced stronger systemic and mucosal immunity. Together, this study proved the high immunogenicity of the CCD/preF vaccines and supported the university of the mucosal CCD adjuvant, supporting further development of the CCD/preF vaccine in larger animal models and clinical studies.https://doi.org/10.1002/mco2.70146respiratory syncytial virus (RSV)intranasal vaccinecrosslinked carbon dot (CCD)adjuvantmucosal immunity |
| spellingShingle | Hong Lei Aqu Alu Jingyun Yang Cai He Jie Shi Weiqi Hong Dandan Peng Yu Zhang Jian Liu Furong Qin Xiya Huang Chunjun Ye Lijiao Pei Xuemei He Hong Yan Guangwen Lu Xiangrong Song Xiawei Wei Yuquan Wei Intranasal Inoculation of Cationic Crosslinked Carbon Dots‐Adjuvanted Respiratory Syncytial Virus F Subunit Vaccine Elicits Mucosal and Systemic Humoral and Cellular Immunity MedComm respiratory syncytial virus (RSV) intranasal vaccine crosslinked carbon dot (CCD) adjuvant mucosal immunity |
| title | Intranasal Inoculation of Cationic Crosslinked Carbon Dots‐Adjuvanted Respiratory Syncytial Virus F Subunit Vaccine Elicits Mucosal and Systemic Humoral and Cellular Immunity |
| title_full | Intranasal Inoculation of Cationic Crosslinked Carbon Dots‐Adjuvanted Respiratory Syncytial Virus F Subunit Vaccine Elicits Mucosal and Systemic Humoral and Cellular Immunity |
| title_fullStr | Intranasal Inoculation of Cationic Crosslinked Carbon Dots‐Adjuvanted Respiratory Syncytial Virus F Subunit Vaccine Elicits Mucosal and Systemic Humoral and Cellular Immunity |
| title_full_unstemmed | Intranasal Inoculation of Cationic Crosslinked Carbon Dots‐Adjuvanted Respiratory Syncytial Virus F Subunit Vaccine Elicits Mucosal and Systemic Humoral and Cellular Immunity |
| title_short | Intranasal Inoculation of Cationic Crosslinked Carbon Dots‐Adjuvanted Respiratory Syncytial Virus F Subunit Vaccine Elicits Mucosal and Systemic Humoral and Cellular Immunity |
| title_sort | intranasal inoculation of cationic crosslinked carbon dots adjuvanted respiratory syncytial virus f subunit vaccine elicits mucosal and systemic humoral and cellular immunity |
| topic | respiratory syncytial virus (RSV) intranasal vaccine crosslinked carbon dot (CCD) adjuvant mucosal immunity |
| url | https://doi.org/10.1002/mco2.70146 |
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