Telomeric expression sites are highly conserved in Trypanosoma brucei.
Subtelomeric regions are often under-represented in genome sequences of eukaryotes. One of the best known examples of the use of telomere proximity for adaptive purposes are the bloodstream expression sites (BESs) of the African trypanosome Trypanosoma brucei. To enhance our understanding of BES str...
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Public Library of Science (PLoS)
2008-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0003527&type=printable |
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| author | Christiane Hertz-Fowler Luisa M Figueiredo Michael A Quail Marion Becker Andrew Jackson Nathalie Bason Karen Brooks Carol Churcher Samah Fahkro Ian Goodhead Paul Heath Magdalena Kartvelishvili Karen Mungall David Harris Heidi Hauser Mandy Sanders David Saunders Kathy Seeger Sarah Sharp Jesse E Taylor Danielle Walker Brian White Rosanna Young George A M Cross Gloria Rudenko J David Barry Edward J Louis Matthew Berriman |
| author_facet | Christiane Hertz-Fowler Luisa M Figueiredo Michael A Quail Marion Becker Andrew Jackson Nathalie Bason Karen Brooks Carol Churcher Samah Fahkro Ian Goodhead Paul Heath Magdalena Kartvelishvili Karen Mungall David Harris Heidi Hauser Mandy Sanders David Saunders Kathy Seeger Sarah Sharp Jesse E Taylor Danielle Walker Brian White Rosanna Young George A M Cross Gloria Rudenko J David Barry Edward J Louis Matthew Berriman |
| author_sort | Christiane Hertz-Fowler |
| collection | DOAJ |
| description | Subtelomeric regions are often under-represented in genome sequences of eukaryotes. One of the best known examples of the use of telomere proximity for adaptive purposes are the bloodstream expression sites (BESs) of the African trypanosome Trypanosoma brucei. To enhance our understanding of BES structure and function in host adaptation and immune evasion, the BES repertoire from the Lister 427 strain of T. brucei were independently tagged and sequenced. BESs are polymorphic in size and structure but reveal a surprisingly conserved architecture in the context of extensive recombination. Very small BESs do exist and many functioning BESs do not contain the full complement of expression site associated genes (ESAGs). The consequences of duplicated or missing ESAGs, including ESAG9, a newly named ESAG12, and additional variant surface glycoprotein genes (VSGs) were evaluated by functional assays after BESs were tagged with a drug-resistance gene. Phylogenetic analysis of constituent ESAG families suggests that BESs are sequence mosaics and that extensive recombination has shaped the evolution of the BES repertoire. This work opens important perspectives in understanding the molecular mechanisms of antigenic variation, a widely used strategy for immune evasion in pathogens, and telomere biology. |
| format | Article |
| id | doaj-art-1c78e94d79a84b92a201a3323f15f31e |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-1c78e94d79a84b92a201a3323f15f31e2025-08-20T02:38:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-01310e352710.1371/journal.pone.0003527Telomeric expression sites are highly conserved in Trypanosoma brucei.Christiane Hertz-FowlerLuisa M FigueiredoMichael A QuailMarion BeckerAndrew JacksonNathalie BasonKaren BrooksCarol ChurcherSamah FahkroIan GoodheadPaul HeathMagdalena KartvelishviliKaren MungallDavid HarrisHeidi HauserMandy SandersDavid SaundersKathy SeegerSarah SharpJesse E TaylorDanielle WalkerBrian WhiteRosanna YoungGeorge A M CrossGloria RudenkoJ David BarryEdward J LouisMatthew BerrimanSubtelomeric regions are often under-represented in genome sequences of eukaryotes. One of the best known examples of the use of telomere proximity for adaptive purposes are the bloodstream expression sites (BESs) of the African trypanosome Trypanosoma brucei. To enhance our understanding of BES structure and function in host adaptation and immune evasion, the BES repertoire from the Lister 427 strain of T. brucei were independently tagged and sequenced. BESs are polymorphic in size and structure but reveal a surprisingly conserved architecture in the context of extensive recombination. Very small BESs do exist and many functioning BESs do not contain the full complement of expression site associated genes (ESAGs). The consequences of duplicated or missing ESAGs, including ESAG9, a newly named ESAG12, and additional variant surface glycoprotein genes (VSGs) were evaluated by functional assays after BESs were tagged with a drug-resistance gene. Phylogenetic analysis of constituent ESAG families suggests that BESs are sequence mosaics and that extensive recombination has shaped the evolution of the BES repertoire. This work opens important perspectives in understanding the molecular mechanisms of antigenic variation, a widely used strategy for immune evasion in pathogens, and telomere biology.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0003527&type=printable |
| spellingShingle | Christiane Hertz-Fowler Luisa M Figueiredo Michael A Quail Marion Becker Andrew Jackson Nathalie Bason Karen Brooks Carol Churcher Samah Fahkro Ian Goodhead Paul Heath Magdalena Kartvelishvili Karen Mungall David Harris Heidi Hauser Mandy Sanders David Saunders Kathy Seeger Sarah Sharp Jesse E Taylor Danielle Walker Brian White Rosanna Young George A M Cross Gloria Rudenko J David Barry Edward J Louis Matthew Berriman Telomeric expression sites are highly conserved in Trypanosoma brucei. PLoS ONE |
| title | Telomeric expression sites are highly conserved in Trypanosoma brucei. |
| title_full | Telomeric expression sites are highly conserved in Trypanosoma brucei. |
| title_fullStr | Telomeric expression sites are highly conserved in Trypanosoma brucei. |
| title_full_unstemmed | Telomeric expression sites are highly conserved in Trypanosoma brucei. |
| title_short | Telomeric expression sites are highly conserved in Trypanosoma brucei. |
| title_sort | telomeric expression sites are highly conserved in trypanosoma brucei |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0003527&type=printable |
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