Both CD4+ and CD8+ Lymphocytes Participate in the IFN-γ Response to Filamentous Hemagglutinin from Bordetella pertussis in Infants, Children, and Adults
Infant CD4+ T-cell responses to bacterial infections or vaccines have been extensively studied, whereas studies on CD8+ T-cell responses focused mainly on viral and intracellular parasite infections. Here we investigated CD8+ T-cell responses upon Bordetella pertussis infection in infants, children,...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/795958 |
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| author | Violette Dirix Virginie Verscheure Françoise Vermeulen Iris De Schutter Tessa Goetghebuer Camille Locht Françoise Mascart |
| author_facet | Violette Dirix Virginie Verscheure Françoise Vermeulen Iris De Schutter Tessa Goetghebuer Camille Locht Françoise Mascart |
| author_sort | Violette Dirix |
| collection | DOAJ |
| description | Infant CD4+ T-cell responses to bacterial infections or vaccines have been extensively studied, whereas studies on CD8+ T-cell responses focused mainly on viral and intracellular parasite infections. Here we investigated CD8+ T-cell responses upon Bordetella pertussis infection in infants, children, and adults and pertussis vaccination in infants. Filamentous hemagglutinin-specific IFN-γ secretion by circulating lymphocytes was blocked by anti-MHC-I or -MHC-II antibodies, suggesting that CD4+ and CD8+ T lymphocytes are involved in IFN-γ production. Flow cytometry analyses confirmed that both cell types synthesized antigen-specific IFN-γ, although CD4+ lymphocytes were the major source of this cytokine. IFN-γ synthesis by CD8+ cells was CD4+ T cell dependent, as evidenced by selective depletion experiments. Furthermore, IFN-γ synthesis by CD4+ cells was sometimes inhibited by CD8+ lymphocytes, suggesting the presence of CD8+ regulatory T cells. The role of this dual IFN-γ secretion by CD4+ and CD8+ T lymphocytes in pertussis remains to be investigated. |
| format | Article |
| id | doaj-art-1c75d34bf0694e7aaca9779c37d836d2 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-1c75d34bf0694e7aaca9779c37d836d22025-02-03T01:03:29ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/795958795958Both CD4+ and CD8+ Lymphocytes Participate in the IFN-γ Response to Filamentous Hemagglutinin from Bordetella pertussis in Infants, Children, and AdultsViolette Dirix0Virginie Verscheure1Françoise Vermeulen2Iris De Schutter3Tessa Goetghebuer4Camille Locht5Françoise Mascart6Laboratory of Vaccinology and Mucosal Immunity, Université Libre de Bruxelles (ULB), 1070 Brussels, BelgiumLaboratory of Vaccinology and Mucosal Immunity, Université Libre de Bruxelles (ULB), 1070 Brussels, BelgiumPediatric Department, Hôpital Erasme, Université Libre de Bruxelles (ULB), 1070 Brussels, BelgiumPediatric Department, UZ Brussel, Vrije Universiteit Brussel (VUB), 1090 Brussels, BelgiumPediatric Department, Hôpital Saint-Pierre, Université Libre de Bruxelles (ULB), 1000 Brussels, BelgiumINSERM U1019, 59000 Lille, FranceLaboratory of Vaccinology and Mucosal Immunity, Université Libre de Bruxelles (ULB), 1070 Brussels, BelgiumInfant CD4+ T-cell responses to bacterial infections or vaccines have been extensively studied, whereas studies on CD8+ T-cell responses focused mainly on viral and intracellular parasite infections. Here we investigated CD8+ T-cell responses upon Bordetella pertussis infection in infants, children, and adults and pertussis vaccination in infants. Filamentous hemagglutinin-specific IFN-γ secretion by circulating lymphocytes was blocked by anti-MHC-I or -MHC-II antibodies, suggesting that CD4+ and CD8+ T lymphocytes are involved in IFN-γ production. Flow cytometry analyses confirmed that both cell types synthesized antigen-specific IFN-γ, although CD4+ lymphocytes were the major source of this cytokine. IFN-γ synthesis by CD8+ cells was CD4+ T cell dependent, as evidenced by selective depletion experiments. Furthermore, IFN-γ synthesis by CD4+ cells was sometimes inhibited by CD8+ lymphocytes, suggesting the presence of CD8+ regulatory T cells. The role of this dual IFN-γ secretion by CD4+ and CD8+ T lymphocytes in pertussis remains to be investigated.http://dx.doi.org/10.1155/2012/795958 |
| spellingShingle | Violette Dirix Virginie Verscheure Françoise Vermeulen Iris De Schutter Tessa Goetghebuer Camille Locht Françoise Mascart Both CD4+ and CD8+ Lymphocytes Participate in the IFN-γ Response to Filamentous Hemagglutinin from Bordetella pertussis in Infants, Children, and Adults Clinical and Developmental Immunology |
| title | Both CD4+ and CD8+ Lymphocytes Participate in the IFN-γ Response to Filamentous Hemagglutinin from Bordetella pertussis in Infants, Children, and Adults |
| title_full | Both CD4+ and CD8+ Lymphocytes Participate in the IFN-γ Response to Filamentous Hemagglutinin from Bordetella pertussis in Infants, Children, and Adults |
| title_fullStr | Both CD4+ and CD8+ Lymphocytes Participate in the IFN-γ Response to Filamentous Hemagglutinin from Bordetella pertussis in Infants, Children, and Adults |
| title_full_unstemmed | Both CD4+ and CD8+ Lymphocytes Participate in the IFN-γ Response to Filamentous Hemagglutinin from Bordetella pertussis in Infants, Children, and Adults |
| title_short | Both CD4+ and CD8+ Lymphocytes Participate in the IFN-γ Response to Filamentous Hemagglutinin from Bordetella pertussis in Infants, Children, and Adults |
| title_sort | both cd4 and cd8 lymphocytes participate in the ifn γ response to filamentous hemagglutinin from bordetella pertussis in infants children and adults |
| url | http://dx.doi.org/10.1155/2012/795958 |
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