<i>Endarachne binghamiae</i> Extract Ameliorates Inflammatory Responses in Macrophages Through Regulation of MAPK, NF-kB and PI3K/AKT Pathways, and Prevents Acute Lung Injury in Mice
In this study, the anti-inflammatory effect of the hot water extract of Endarachne binghamiae (EB-WE), a type of marine brown algae, was investigated in LPS-stimulated RAW 264.7 cells and an acute lung injury (ALI) mouse model induced by intranasal LPS administration. Treatment with EB-WE significan...
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2025-01-01
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| author | Sang-Hoon Lee Sang-Seop Lee Ga-Young Lee Seung-Yun Han Dong-Sub Kim Bong-Ho Lee Yung-Choon Yoo |
| author_facet | Sang-Hoon Lee Sang-Seop Lee Ga-Young Lee Seung-Yun Han Dong-Sub Kim Bong-Ho Lee Yung-Choon Yoo |
| author_sort | Sang-Hoon Lee |
| collection | DOAJ |
| description | In this study, the anti-inflammatory effect of the hot water extract of Endarachne binghamiae (EB-WE), a type of marine brown algae, was investigated in LPS-stimulated RAW 264.7 cells and an acute lung injury (ALI) mouse model induced by intranasal LPS administration. Treatment with EB-WE significantly inhibited NO and pro-inflammatory cytokine (TNF-a and IL-6) production in LPS-stimulated RAW 264.7 cells. In mRNA analysis, the expression of pro-inflammatory cytokines, COX-2, and iNOS mRNAs, was down-regulated by EB-WE treatment. The phosphorylation of MAPK, IkB, and PI3K/AKT molecules responsible for signal pathways during inflammation in LPS-stimulated macrophages was also significantly inhibited by EB-WE. In an in vivo model for ALI, oral administration of EB-WE significantly reduced the level of pro-inflammatory cytokines (TNF-a, IL-1b, and IL-6) and chemokines (MCP-1, CXC-16, CXCL1, and TARC) in serum or bronchoalveolar lavage fluid (BALF) of mice. Similarly to the results in LPS-stimulated RAW 264.7 cells, treatment with EB-WE significantly inhibited intracellular signal pathways mediated by MAPK, IkB, and PI3K/AKT in lung tissues of mice with ALI, and also decreased the expression of mRNAs of inflammatory mediators such as TNF-a, IL-6, iNOS, and COX-2. Furthermore, the inhibitory effect of EB-WE on ALI was apparently confirmed in histological examination through lung tissue staining. Taken together, it is clear that EB-WE has potential activity to effectively ameliorate the inflammatory responses in macrophages through down-regulation of MAPK, NF-kB, and PI3K/AKT activation, and suppress acute lung injury induced by LPS. These findings strongly suggest that EB-WE is a promising natural product beneficial for developing preventive treatments and cures of inflammation-related diseases. |
| format | Article |
| id | doaj-art-1c2357c821884bc09dc651fc6e253178 |
| institution | Kabale University |
| issn | 2075-1729 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj-art-1c2357c821884bc09dc651fc6e2531782025-01-24T13:38:44ZengMDPI AGLife2075-17292025-01-011518810.3390/life15010088<i>Endarachne binghamiae</i> Extract Ameliorates Inflammatory Responses in Macrophages Through Regulation of MAPK, NF-kB and PI3K/AKT Pathways, and Prevents Acute Lung Injury in MiceSang-Hoon Lee0Sang-Seop Lee1Ga-Young Lee2Seung-Yun Han3Dong-Sub Kim4Bong-Ho Lee5Yung-Choon Yoo6Department of Microbiology, College of Medicine, Konyang University, Daejeon 32992, Republic of KoreaDepartment of Microbiology, College of Medicine, Konyang University, Daejeon 32992, Republic of KoreaDepartment of Microbiology, College of Medicine, Konyang University, Daejeon 32992, Republic of KoreaDepartment of Anatomy, College of Medicine, Konyang University, Daejeon 32992, Republic of KoreaDivision of Natural Product Research, Korea Prime Pharmacy Co., Ltd., Gwangju 58144, Republic of KoreaDepartment of Chemical Technology, Hanbat National University, Daejeon 34158, Republic of KoreaDepartment of Microbiology, College of Medicine, Konyang University, Daejeon 32992, Republic of KoreaIn this study, the anti-inflammatory effect of the hot water extract of Endarachne binghamiae (EB-WE), a type of marine brown algae, was investigated in LPS-stimulated RAW 264.7 cells and an acute lung injury (ALI) mouse model induced by intranasal LPS administration. Treatment with EB-WE significantly inhibited NO and pro-inflammatory cytokine (TNF-a and IL-6) production in LPS-stimulated RAW 264.7 cells. In mRNA analysis, the expression of pro-inflammatory cytokines, COX-2, and iNOS mRNAs, was down-regulated by EB-WE treatment. The phosphorylation of MAPK, IkB, and PI3K/AKT molecules responsible for signal pathways during inflammation in LPS-stimulated macrophages was also significantly inhibited by EB-WE. In an in vivo model for ALI, oral administration of EB-WE significantly reduced the level of pro-inflammatory cytokines (TNF-a, IL-1b, and IL-6) and chemokines (MCP-1, CXC-16, CXCL1, and TARC) in serum or bronchoalveolar lavage fluid (BALF) of mice. Similarly to the results in LPS-stimulated RAW 264.7 cells, treatment with EB-WE significantly inhibited intracellular signal pathways mediated by MAPK, IkB, and PI3K/AKT in lung tissues of mice with ALI, and also decreased the expression of mRNAs of inflammatory mediators such as TNF-a, IL-6, iNOS, and COX-2. Furthermore, the inhibitory effect of EB-WE on ALI was apparently confirmed in histological examination through lung tissue staining. Taken together, it is clear that EB-WE has potential activity to effectively ameliorate the inflammatory responses in macrophages through down-regulation of MAPK, NF-kB, and PI3K/AKT activation, and suppress acute lung injury induced by LPS. These findings strongly suggest that EB-WE is a promising natural product beneficial for developing preventive treatments and cures of inflammation-related diseases.https://www.mdpi.com/2075-1729/15/1/88<i>Endarachne binghamiae</i>inflammationacute lung injuryMAPKNF-kB |
| spellingShingle | Sang-Hoon Lee Sang-Seop Lee Ga-Young Lee Seung-Yun Han Dong-Sub Kim Bong-Ho Lee Yung-Choon Yoo <i>Endarachne binghamiae</i> Extract Ameliorates Inflammatory Responses in Macrophages Through Regulation of MAPK, NF-kB and PI3K/AKT Pathways, and Prevents Acute Lung Injury in Mice Life <i>Endarachne binghamiae</i> inflammation acute lung injury MAPK NF-kB |
| title | <i>Endarachne binghamiae</i> Extract Ameliorates Inflammatory Responses in Macrophages Through Regulation of MAPK, NF-kB and PI3K/AKT Pathways, and Prevents Acute Lung Injury in Mice |
| title_full | <i>Endarachne binghamiae</i> Extract Ameliorates Inflammatory Responses in Macrophages Through Regulation of MAPK, NF-kB and PI3K/AKT Pathways, and Prevents Acute Lung Injury in Mice |
| title_fullStr | <i>Endarachne binghamiae</i> Extract Ameliorates Inflammatory Responses in Macrophages Through Regulation of MAPK, NF-kB and PI3K/AKT Pathways, and Prevents Acute Lung Injury in Mice |
| title_full_unstemmed | <i>Endarachne binghamiae</i> Extract Ameliorates Inflammatory Responses in Macrophages Through Regulation of MAPK, NF-kB and PI3K/AKT Pathways, and Prevents Acute Lung Injury in Mice |
| title_short | <i>Endarachne binghamiae</i> Extract Ameliorates Inflammatory Responses in Macrophages Through Regulation of MAPK, NF-kB and PI3K/AKT Pathways, and Prevents Acute Lung Injury in Mice |
| title_sort | i endarachne binghamiae i extract ameliorates inflammatory responses in macrophages through regulation of mapk nf kb and pi3k akt pathways and prevents acute lung injury in mice |
| topic | <i>Endarachne binghamiae</i> inflammation acute lung injury MAPK NF-kB |
| url | https://www.mdpi.com/2075-1729/15/1/88 |
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