Limitations of methods for measuring the concentration of human genomic DNA and oligonucleotide samples

We compared different methods (absorbance, fluorescent dye-binding, and digital PCR) for measuring the concentrations of human genomic DNA from cultured cells and absorbance measurements of a synthetic DNA oligonucleotide. NIST Standard Reference Material (SRM) 2082, a pathlength absorbance standard...

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Bibliographic Details
Main Authors: Hua-Jun He, Erica V. Stein, Paul DeRose, Kenneth D. Cole
Format: Article
Language:English
Published: Taylor & Francis Group 2018-02-01
Series:BioTechniques
Subjects:
Online Access:https://www.future-science.com/doi/10.2144/btn-2017-0102
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