Detection and Genetic Analysis of Porcine Bocavirus in Different Swine Herds in North Central China
Porcine Bocavirus (PBoV) has been reported to be associated with postweaning multisystemic wasting syndrome and pneumonia in pigs. In this study, a survey was conducted to evaluate the prevalence of PBoV in slaughter pigs, sick pigs, asymptomatic pigs and classical swine fever virus (CSFV) eradicati...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/947084 |
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| author | Mengmeng Liu Yi Li Dong Sun Yihe Xia Jinhai Huang Lili Guo |
| author_facet | Mengmeng Liu Yi Li Dong Sun Yihe Xia Jinhai Huang Lili Guo |
| author_sort | Mengmeng Liu |
| collection | DOAJ |
| description | Porcine Bocavirus (PBoV) has been reported to be associated with postweaning multisystemic wasting syndrome and pneumonia in pigs. In this study, a survey was conducted to evaluate the prevalence of PBoV in slaughter pigs, sick pigs, asymptomatic pigs and classical swine fever virus (CSFV) eradication plan herds in five provinces of China (Henan, Liaoning, Shandong, Hebei and Tianjin) by means of PCR targeting NS1 gene of PBoV. Among the total of 403 tissue samples, 11.41% were positive for PBoV. The positive rates of spleen (20.75%) and inguinal lymph node (27.18%) are higher than those of other organs. PCR products of twenty PBoV positive samples from slaughter pigs were sequenced for phylogenetic analysis. The result revealed that PBoV could be divided into 6 groups (PBoV-a~PBoV-f). All PBoV sequenced in this study belong to PBoV-a–PBoV-d with 90.1% to 99% nucleotide identities. Our results exhibited significant genetic diversity of PBoV and suggested a complex prevalence of PBoV in Chinese swine herds. Whether this diversity of PBoV has a significance to pig production or even public health remains to be further studied. |
| format | Article |
| id | doaj-art-1bf0e5576e0942198b7e35d87d965cff |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-1bf0e5576e0942198b7e35d87d965cff2025-02-03T01:11:20ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/947084947084Detection and Genetic Analysis of Porcine Bocavirus in Different Swine Herds in North Central ChinaMengmeng Liu0Yi Li1Dong Sun2Yihe Xia3Jinhai Huang4Lili Guo5School of Chemical Engineering and Technology, Tianjin University, No. 92, Weijin Road, Nankai District, Tianjin 300072, ChinaSchool of Chemical Engineering and Technology, Tianjin University, No. 92, Weijin Road, Nankai District, Tianjin 300072, ChinaDepartment of Veterinary Diagnosis and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USASchool of Chemical Engineering and Technology, Tianjin University, No. 92, Weijin Road, Nankai District, Tianjin 300072, ChinaSchool of Chemical Engineering and Technology, Tianjin University, No. 92, Weijin Road, Nankai District, Tianjin 300072, ChinaTianjin Center of Animal Disease Preventive and Control, Tianjin 300012, ChinaPorcine Bocavirus (PBoV) has been reported to be associated with postweaning multisystemic wasting syndrome and pneumonia in pigs. In this study, a survey was conducted to evaluate the prevalence of PBoV in slaughter pigs, sick pigs, asymptomatic pigs and classical swine fever virus (CSFV) eradication plan herds in five provinces of China (Henan, Liaoning, Shandong, Hebei and Tianjin) by means of PCR targeting NS1 gene of PBoV. Among the total of 403 tissue samples, 11.41% were positive for PBoV. The positive rates of spleen (20.75%) and inguinal lymph node (27.18%) are higher than those of other organs. PCR products of twenty PBoV positive samples from slaughter pigs were sequenced for phylogenetic analysis. The result revealed that PBoV could be divided into 6 groups (PBoV-a~PBoV-f). All PBoV sequenced in this study belong to PBoV-a–PBoV-d with 90.1% to 99% nucleotide identities. Our results exhibited significant genetic diversity of PBoV and suggested a complex prevalence of PBoV in Chinese swine herds. Whether this diversity of PBoV has a significance to pig production or even public health remains to be further studied.http://dx.doi.org/10.1155/2014/947084 |
| spellingShingle | Mengmeng Liu Yi Li Dong Sun Yihe Xia Jinhai Huang Lili Guo Detection and Genetic Analysis of Porcine Bocavirus in Different Swine Herds in North Central China The Scientific World Journal |
| title | Detection and Genetic Analysis of Porcine Bocavirus in Different Swine Herds in North Central China |
| title_full | Detection and Genetic Analysis of Porcine Bocavirus in Different Swine Herds in North Central China |
| title_fullStr | Detection and Genetic Analysis of Porcine Bocavirus in Different Swine Herds in North Central China |
| title_full_unstemmed | Detection and Genetic Analysis of Porcine Bocavirus in Different Swine Herds in North Central China |
| title_short | Detection and Genetic Analysis of Porcine Bocavirus in Different Swine Herds in North Central China |
| title_sort | detection and genetic analysis of porcine bocavirus in different swine herds in north central china |
| url | http://dx.doi.org/10.1155/2014/947084 |
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