Osteoblastic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells on P3HT Thin Polymer Film
Bone defects restoration has always been an arduous challenge in the orthopedic field due to the limitations of conventional grafts. Bone tissue engineering offers an alternative approach by using biomimetic materials, stem cells, and growth factors that are able to improve the regeneration of bone...
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MDPI AG
2025-01-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/16/1/10 |
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| author | Paola Campione Maria Giovanna Rizzo Luana Vittoria Bauso Ileana Ielo Grazia Maria Lucia Messina Giovanna Calabrese |
| author_facet | Paola Campione Maria Giovanna Rizzo Luana Vittoria Bauso Ileana Ielo Grazia Maria Lucia Messina Giovanna Calabrese |
| author_sort | Paola Campione |
| collection | DOAJ |
| description | Bone defects restoration has always been an arduous challenge in the orthopedic field due to the limitations of conventional grafts. Bone tissue engineering offers an alternative approach by using biomimetic materials, stem cells, and growth factors that are able to improve the regeneration of bone tissue. Different biomaterials have attracted great interest in BTE applications, including the poly(3-hexylthiofene) (P3HT) conductive polymer, whose primary advantage is its capability to provide a native extracellular matrix-like environment. Based on this evidence, in this study, we evaluated the biological response of human adipose-derived mesenchymal stem cells cultured on P3HT thin polymer film for 14 days. Our results suggest that P3HT represents a good substrate to induce osteogenic differentiation of osteoprogenitor cells, even in the absence of specific inductive growth factors, thus representing a promising strategy for bone regenerative medicine. Therefore, the system provided may offer an innovative platform for next-generation biocompatible materials for regenerative medicine. |
| format | Article |
| id | doaj-art-1bed09444f4c433ebe1ec0dc435e28f6 |
| institution | Kabale University |
| issn | 2079-4983 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj-art-1bed09444f4c433ebe1ec0dc435e28f62025-01-24T13:36:06ZengMDPI AGJournal of Functional Biomaterials2079-49832025-01-011611010.3390/jfb16010010Osteoblastic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells on P3HT Thin Polymer FilmPaola Campione0Maria Giovanna Rizzo1Luana Vittoria Bauso2Ileana Ielo3Grazia Maria Lucia Messina4Giovanna Calabrese5Department of Chemical Sciences, University of Catania and CSGI, Viale A. Doria 6, 95125 Catania, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences (ChiBioFarAm), University of Messina, Viale F. Stagno d’Alcontres 31, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences (ChiBioFarAm), University of Messina, Viale F. Stagno d’Alcontres 31, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences (ChiBioFarAm), University of Messina, Viale F. Stagno d’Alcontres 31, 98166 Messina, ItalyDepartment of Chemical Sciences, University of Catania and CSGI, Viale A. Doria 6, 95125 Catania, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences (ChiBioFarAm), University of Messina, Viale F. Stagno d’Alcontres 31, 98166 Messina, ItalyBone defects restoration has always been an arduous challenge in the orthopedic field due to the limitations of conventional grafts. Bone tissue engineering offers an alternative approach by using biomimetic materials, stem cells, and growth factors that are able to improve the regeneration of bone tissue. Different biomaterials have attracted great interest in BTE applications, including the poly(3-hexylthiofene) (P3HT) conductive polymer, whose primary advantage is its capability to provide a native extracellular matrix-like environment. Based on this evidence, in this study, we evaluated the biological response of human adipose-derived mesenchymal stem cells cultured on P3HT thin polymer film for 14 days. Our results suggest that P3HT represents a good substrate to induce osteogenic differentiation of osteoprogenitor cells, even in the absence of specific inductive growth factors, thus representing a promising strategy for bone regenerative medicine. Therefore, the system provided may offer an innovative platform for next-generation biocompatible materials for regenerative medicine.https://www.mdpi.com/2079-4983/16/1/10bone tissue engineeringsemiconductive polymerscytocompatibilityosteoconductivityosteoinductivity |
| spellingShingle | Paola Campione Maria Giovanna Rizzo Luana Vittoria Bauso Ileana Ielo Grazia Maria Lucia Messina Giovanna Calabrese Osteoblastic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells on P3HT Thin Polymer Film Journal of Functional Biomaterials bone tissue engineering semiconductive polymers cytocompatibility osteoconductivity osteoinductivity |
| title | Osteoblastic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells on P3HT Thin Polymer Film |
| title_full | Osteoblastic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells on P3HT Thin Polymer Film |
| title_fullStr | Osteoblastic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells on P3HT Thin Polymer Film |
| title_full_unstemmed | Osteoblastic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells on P3HT Thin Polymer Film |
| title_short | Osteoblastic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells on P3HT Thin Polymer Film |
| title_sort | osteoblastic differentiation of human adipose derived mesenchymal stem cells on p3ht thin polymer film |
| topic | bone tissue engineering semiconductive polymers cytocompatibility osteoconductivity osteoinductivity |
| url | https://www.mdpi.com/2079-4983/16/1/10 |
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