Variation in mitochondrial genome primary sequence among whole-genome-sequenced strains of Neurospora crassa
Eighteen classical mutant strains of Neurospora crassa were subject to whole genome sequence analysis and the mitochondrial genome is analyzed. Overall, the mitochondrial genomes of the classical mutant strains are 99.45 to 99.98 % identical to the reference genome. Two-thirds of the SNPs and three...
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BMC
2012-06-01
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| Series: | IMA Fungus |
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| author | K. McCluskey |
| author_facet | K. McCluskey |
| author_sort | K. McCluskey |
| collection | DOAJ |
| description | Eighteen classical mutant strains of Neurospora crassa were subject to whole genome sequence analysis and the mitochondrial genome is analyzed. Overall, the mitochondrial genomes of the classical mutant strains are 99.45 to 99.98 % identical to the reference genome. Two-thirds of the SNPs and three-fourths of indels identified in this analysis are shared among more than one strain. Most of the limited variability in mitochondrial genome sequence is neutral with regard to protein structure. Despite the fact that the mitochondrial genome is present in multiple copies per cell, many of the polymorphisms were homozygous within each strain. Conversely, some polymorphisms, especially those associated with large scale rearrangements are only present in a fraction of the reads covering each region. The impact of this variation is unknown and further studies will be necessary to ascertain if this level of polymorphism is common among fungi and whether it reflects the impact of ageing cultures. |
| format | Article |
| id | doaj-art-1bb065ef16bb4a148b941085b0412296 |
| institution | Kabale University |
| issn | 2210-6340 2210-6359 |
| language | English |
| publishDate | 2012-06-01 |
| publisher | BMC |
| record_format | Article |
| series | IMA Fungus |
| spelling | doaj-art-1bb065ef16bb4a148b941085b04122962025-02-02T20:43:27ZengBMCIMA Fungus2210-63402210-63592012-06-01319398Variation in mitochondrial genome primary sequence among whole-genome-sequenced strains of Neurospora crassaK. McCluskeyEighteen classical mutant strains of Neurospora crassa were subject to whole genome sequence analysis and the mitochondrial genome is analyzed. Overall, the mitochondrial genomes of the classical mutant strains are 99.45 to 99.98 % identical to the reference genome. Two-thirds of the SNPs and three-fourths of indels identified in this analysis are shared among more than one strain. Most of the limited variability in mitochondrial genome sequence is neutral with regard to protein structure. Despite the fact that the mitochondrial genome is present in multiple copies per cell, many of the polymorphisms were homozygous within each strain. Conversely, some polymorphisms, especially those associated with large scale rearrangements are only present in a fraction of the reads covering each region. The impact of this variation is unknown and further studies will be necessary to ascertain if this level of polymorphism is common among fungi and whether it reflects the impact of ageing cultures.http://www.ingentaconnect.com/content/ima/imafung/2012/00000003/00000001/art00022SNPIndelsequence polymorphismorganelle genomeclassical mutant |
| spellingShingle | K. McCluskey Variation in mitochondrial genome primary sequence among whole-genome-sequenced strains of Neurospora crassa IMA Fungus SNP Indel sequence polymorphism organelle genome classical mutant |
| title | Variation in mitochondrial genome primary sequence among whole-genome-sequenced strains of Neurospora crassa |
| title_full | Variation in mitochondrial genome primary sequence among whole-genome-sequenced strains of Neurospora crassa |
| title_fullStr | Variation in mitochondrial genome primary sequence among whole-genome-sequenced strains of Neurospora crassa |
| title_full_unstemmed | Variation in mitochondrial genome primary sequence among whole-genome-sequenced strains of Neurospora crassa |
| title_short | Variation in mitochondrial genome primary sequence among whole-genome-sequenced strains of Neurospora crassa |
| title_sort | variation in mitochondrial genome primary sequence among whole genome sequenced strains of neurospora crassa |
| topic | SNP Indel sequence polymorphism organelle genome classical mutant |
| url | http://www.ingentaconnect.com/content/ima/imafung/2012/00000003/00000001/art00022 |
| work_keys_str_mv | AT kmccluskey variationinmitochondrialgenomeprimarysequenceamongwholegenomesequencedstrainsofneurosporacrassa |