Isolation of group B Streptococcus with reduced β-lactam susceptibility from pregnant women
β-Lactam antibiotics are first-line agents for the treatment and prevention of group B Streptococcus (GBS) infections. We previously reported clinical GBS isolates with reduced β-lactam susceptibility (GBS-RBS) and characterized them as harbouring amino acid substitutions in penicillin-binding prote...
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Taylor & Francis Group
2019-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2018.1557987 |
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| author | Hiroaki Moroi Kouji Kimura Tomomi Kotani Hiroyuki Tsuda Hirotsugu Banno Wanchun Jin Jun-ichi Wachino Keiko Yamada Takashi Mitsui Mamoru Yamashita Fumitaka Kikkawa Yoshichika Arakawa |
| author_facet | Hiroaki Moroi Kouji Kimura Tomomi Kotani Hiroyuki Tsuda Hirotsugu Banno Wanchun Jin Jun-ichi Wachino Keiko Yamada Takashi Mitsui Mamoru Yamashita Fumitaka Kikkawa Yoshichika Arakawa |
| author_sort | Hiroaki Moroi |
| collection | DOAJ |
| description | β-Lactam antibiotics are first-line agents for the treatment and prevention of group B Streptococcus (GBS) infections. We previously reported clinical GBS isolates with reduced β-lactam susceptibility (GBS-RBS) and characterized them as harbouring amino acid substitutions in penicillin-binding proteins (PBPs). However, to our knowledge, GBS-RBS clinical isolates have never previously been isolated from pregnant women worldwide. We obtained 477 clinical GBS isolates from vaginal/rectal swabs of 4530 pregnant women in Japan. We determined the MICs of seven β-lactams for all 477 clinical isolates. Five clinical isolates showed reduced ceftibuten susceptibility. For these isolates, we performed sequencing analysis of pbp genes. None of the 477 isolates were non-susceptible to penicillin G, ampicillin, and meropenem. For five isolates, the MICs of ceftibuten were relatively high (64–128 μg/ml). Each of these isolates possessed a single amino acid substitution in PBP2X, and some of the substitutions had been previously found in GBS with reduced penicillin susceptibility. This is the first report of the isolation of clinical GBS-RBS isolates harbouring amino acid substitutions in PBP2X that confer reduced ceftibuten susceptibility from pregnant women. |
| format | Article |
| id | doaj-art-1baee73f16744ffda8dc65606ffda855 |
| institution | OA Journals |
| issn | 2222-1751 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-1baee73f16744ffda8dc65606ffda8552025-08-20T02:08:12ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512019-01-01812710.1080/22221751.2018.1557987Isolation of group B Streptococcus with reduced β-lactam susceptibility from pregnant womenHiroaki Moroi0Kouji Kimura1Tomomi Kotani2Hiroyuki Tsuda3Hirotsugu Banno4Wanchun Jin5Jun-ichi Wachino6Keiko Yamada7Takashi Mitsui8Mamoru Yamashita9Fumitaka Kikkawa10Yoshichika Arakawa11Department of Bacteriology, Nagoya University Graduate School of Medicine, Nagoya, JapanDepartment of Bacteriology, Nagoya University Graduate School of Medicine, Nagoya, JapanDepartment of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Nagoya, JapanDepartment of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Nagoya, JapanDepartment of Bacteriology, Nagoya University Graduate School of Medicine, Nagoya, JapanDepartment of Bacteriology, Nagoya University Graduate School of Medicine, Nagoya, JapanDepartment of Bacteriology, Nagoya University Graduate School of Medicine, Nagoya, JapanDepartment of Bacteriology, Nagoya University Graduate School of Medicine, Nagoya, JapanKishokai Medical Corporation, Inazawa, JapanKishokai Medical Corporation, Inazawa, JapanDepartment of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Nagoya, JapanDepartment of Bacteriology, Nagoya University Graduate School of Medicine, Nagoya, Japanβ-Lactam antibiotics are first-line agents for the treatment and prevention of group B Streptococcus (GBS) infections. We previously reported clinical GBS isolates with reduced β-lactam susceptibility (GBS-RBS) and characterized them as harbouring amino acid substitutions in penicillin-binding proteins (PBPs). However, to our knowledge, GBS-RBS clinical isolates have never previously been isolated from pregnant women worldwide. We obtained 477 clinical GBS isolates from vaginal/rectal swabs of 4530 pregnant women in Japan. We determined the MICs of seven β-lactams for all 477 clinical isolates. Five clinical isolates showed reduced ceftibuten susceptibility. For these isolates, we performed sequencing analysis of pbp genes. None of the 477 isolates were non-susceptible to penicillin G, ampicillin, and meropenem. For five isolates, the MICs of ceftibuten were relatively high (64–128 μg/ml). Each of these isolates possessed a single amino acid substitution in PBP2X, and some of the substitutions had been previously found in GBS with reduced penicillin susceptibility. This is the first report of the isolation of clinical GBS-RBS isolates harbouring amino acid substitutions in PBP2X that confer reduced ceftibuten susceptibility from pregnant women.https://www.tandfonline.com/doi/10.1080/22221751.2018.1557987Group B StreptococcusGBSStreptococcus agalactiaepregnant womenreduced β-lactam susceptibility |
| spellingShingle | Hiroaki Moroi Kouji Kimura Tomomi Kotani Hiroyuki Tsuda Hirotsugu Banno Wanchun Jin Jun-ichi Wachino Keiko Yamada Takashi Mitsui Mamoru Yamashita Fumitaka Kikkawa Yoshichika Arakawa Isolation of group B Streptococcus with reduced β-lactam susceptibility from pregnant women Emerging Microbes and Infections Group B Streptococcus GBS Streptococcus agalactiae pregnant women reduced β-lactam susceptibility |
| title | Isolation of group B Streptococcus with reduced β-lactam susceptibility from pregnant women |
| title_full | Isolation of group B Streptococcus with reduced β-lactam susceptibility from pregnant women |
| title_fullStr | Isolation of group B Streptococcus with reduced β-lactam susceptibility from pregnant women |
| title_full_unstemmed | Isolation of group B Streptococcus with reduced β-lactam susceptibility from pregnant women |
| title_short | Isolation of group B Streptococcus with reduced β-lactam susceptibility from pregnant women |
| title_sort | isolation of group b streptococcus with reduced β lactam susceptibility from pregnant women |
| topic | Group B Streptococcus GBS Streptococcus agalactiae pregnant women reduced β-lactam susceptibility |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2018.1557987 |
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