Effects of Cytokinins on Morphogenesis, Total (Poly)Phenolic Content and Antioxidant Capacity of In Vitro-Cultured Hop Plantlets, cvs. Cascade and Columbus
(1) Background: <i>Humulus lupulus</i> L. plants constitute a rich source of bioactive compounds. The synthesis of bioactive compounds in plants is often triggered by the activation of secondary metabolism, which can be induced by biotic or abiotic elicitors. In vitro, the effect of the...
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MDPI AG
2025-01-01
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| author | Leandra Leto Valeria Guarrasi Anna Agosti Martina Nironi Benedetta Chiancone Jorge Juan Vicedo |
| author_facet | Leandra Leto Valeria Guarrasi Anna Agosti Martina Nironi Benedetta Chiancone Jorge Juan Vicedo |
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| description | (1) Background: <i>Humulus lupulus</i> L. plants constitute a rich source of bioactive compounds. The synthesis of bioactive compounds in plants is often triggered by the activation of secondary metabolism, which can be induced by biotic or abiotic elicitors. In vitro, the effect of the elicitors can be studied in a controlled environment and in a small space, independently of seasonal variations. Cytokinins are frequently used in plant tissue culture for bud regeneration, branching and shoot elongation due to their role in cell division enhancement. This study aimed to investigate the effects of different cytokinins on the growth parameters, total (poly)phenolic content and antioxidant capacity of in vitro-grown hop plants to evaluate hop vitro-derived biomass as a potential source of bioactive compounds. (2) Methods: unimodal hop (cvs. Cascade and Columbus) explants were cultured on media enriched with four cytokinins (kinetin, 6-benzylaminopurine, meta-topolin and 6-(γ,γ-dimethylallylamino)-purine) at four concentrations. (3) Results: A genotype-dependent response to different cytokinins was encountered. (4) Conclusions Columbus explants could root in culture media auxin-free, providing valuable opportunities for commercial nurseries. Moreover, cytokinins were confirmed to be valuable elicitors to stimulate the bioactive compound biosynthesis in micropropagated hop plants, making them a precious source for various industries. |
| format | Article |
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| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-1b9c6e3c6a4f4459be5d7adb90a2d9a42025-08-20T02:48:07ZengMDPI AGPlants2223-77472025-01-0114341810.3390/plants14030418Effects of Cytokinins on Morphogenesis, Total (Poly)Phenolic Content and Antioxidant Capacity of In Vitro-Cultured Hop Plantlets, cvs. Cascade and ColumbusLeandra Leto0Valeria Guarrasi1Anna Agosti2Martina Nironi3Benedetta Chiancone4Jorge Juan Vicedo5Department of Food and Drug, University of Parma, Viale Parco Area delle Scienze 27/A, 43124 Parma, ItalyInstitute of Biophysics, National Research Council (CNR), Via Ugo La Malfa 153, 90146 Palermo, ItalyDepartment of Food and Drug, University of Parma, Viale Parco Area delle Scienze 27/A, 43124 Parma, ItalyDepartment of Food and Drug, University of Parma, Viale Parco Area delle Scienze 27/A, 43124 Parma, ItalyDepartment of Food and Drug, University of Parma, Viale Parco Area delle Scienze 27/A, 43124 Parma, ItalyInstituto de Investigación en Medio Ambiente y Ciencia Marina IMEDMAR, Universidad Católica de Valencia San Vicente Mártir, Carrer Guillem de Castro, 94, 46001 València, Spain(1) Background: <i>Humulus lupulus</i> L. plants constitute a rich source of bioactive compounds. The synthesis of bioactive compounds in plants is often triggered by the activation of secondary metabolism, which can be induced by biotic or abiotic elicitors. In vitro, the effect of the elicitors can be studied in a controlled environment and in a small space, independently of seasonal variations. Cytokinins are frequently used in plant tissue culture for bud regeneration, branching and shoot elongation due to their role in cell division enhancement. This study aimed to investigate the effects of different cytokinins on the growth parameters, total (poly)phenolic content and antioxidant capacity of in vitro-grown hop plants to evaluate hop vitro-derived biomass as a potential source of bioactive compounds. (2) Methods: unimodal hop (cvs. Cascade and Columbus) explants were cultured on media enriched with four cytokinins (kinetin, 6-benzylaminopurine, meta-topolin and 6-(γ,γ-dimethylallylamino)-purine) at four concentrations. (3) Results: A genotype-dependent response to different cytokinins was encountered. (4) Conclusions Columbus explants could root in culture media auxin-free, providing valuable opportunities for commercial nurseries. Moreover, cytokinins were confirmed to be valuable elicitors to stimulate the bioactive compound biosynthesis in micropropagated hop plants, making them a precious source for various industries.https://www.mdpi.com/2223-7747/14/3/418bioactive compoundsgrowth regulators<i>Humulus lupulus</i> (L.), in vitro propagationsecondary metabolism |
| spellingShingle | Leandra Leto Valeria Guarrasi Anna Agosti Martina Nironi Benedetta Chiancone Jorge Juan Vicedo Effects of Cytokinins on Morphogenesis, Total (Poly)Phenolic Content and Antioxidant Capacity of In Vitro-Cultured Hop Plantlets, cvs. Cascade and Columbus Plants bioactive compounds growth regulators <i>Humulus lupulus</i> (L.), in vitro propagation secondary metabolism |
| title | Effects of Cytokinins on Morphogenesis, Total (Poly)Phenolic Content and Antioxidant Capacity of In Vitro-Cultured Hop Plantlets, cvs. Cascade and Columbus |
| title_full | Effects of Cytokinins on Morphogenesis, Total (Poly)Phenolic Content and Antioxidant Capacity of In Vitro-Cultured Hop Plantlets, cvs. Cascade and Columbus |
| title_fullStr | Effects of Cytokinins on Morphogenesis, Total (Poly)Phenolic Content and Antioxidant Capacity of In Vitro-Cultured Hop Plantlets, cvs. Cascade and Columbus |
| title_full_unstemmed | Effects of Cytokinins on Morphogenesis, Total (Poly)Phenolic Content and Antioxidant Capacity of In Vitro-Cultured Hop Plantlets, cvs. Cascade and Columbus |
| title_short | Effects of Cytokinins on Morphogenesis, Total (Poly)Phenolic Content and Antioxidant Capacity of In Vitro-Cultured Hop Plantlets, cvs. Cascade and Columbus |
| title_sort | effects of cytokinins on morphogenesis total poly phenolic content and antioxidant capacity of in vitro cultured hop plantlets cvs cascade and columbus |
| topic | bioactive compounds growth regulators <i>Humulus lupulus</i> (L.), in vitro propagation secondary metabolism |
| url | https://www.mdpi.com/2223-7747/14/3/418 |
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