The CT-Element of The C-Myc Gene Does Not Predispose to Chromosomal Breakpoints in Burkitt'S Lymphoma
Background: Chromosomal translocations are causally related to the development of many tumors. In Burkitt's lymphoma, abnormalities involving the c-myc gene are essential. The CT-element of the c-myc promoter adopts non-B-conformation in vivo and in vitro, and therefore provides a potential fra...
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| Format: | Article |
| Language: | English |
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Wiley
2006-01-01
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| Series: | Cellular Oncology |
| Online Access: | http://dx.doi.org/10.1155/2006/695262 |
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| author | Achim Weber Marina I. Gutierrez David Levens |
| author_facet | Achim Weber Marina I. Gutierrez David Levens |
| author_sort | Achim Weber |
| collection | DOAJ |
| description | Background: Chromosomal translocations are causally related to the development of many tumors. In Burkitt's lymphoma, abnormalities involving the c-myc gene are essential. The CT-element of the c-myc promoter adopts non-B-conformation in vivo and in vitro, and therefore provides a potential fragile site. Methods: We have developed a LM-PCR-based approach to test if chromosomal breakpoints indeed cluster in this region. Results: Amplifying both, wild-type as well as the translocated c-myc gene by LM-PCR, it was shown that chromosomal breakpoints did not cluster within the CT-element. Conclusions: Therefore, the CT-element is not especially susceptible to the formation of breakpoints leading to chromosomal translocations in Burkitt's lymphoma. |
| format | Article |
| id | doaj-art-1b64a5763e3347d3ae43f60b05df9871 |
| institution | Kabale University |
| issn | 1570-5870 1875-8606 |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cellular Oncology |
| spelling | doaj-art-1b64a5763e3347d3ae43f60b05df98712025-08-20T03:54:23ZengWileyCellular Oncology1570-58701875-86062006-01-01281-2313510.1155/2006/695262The CT-Element of The C-Myc Gene Does Not Predispose to Chromosomal Breakpoints in Burkitt'S LymphomaAchim Weber0Marina I. Gutierrez1David Levens2Institute of Pathology, Johannes Gutenberg-University of Mainz, Langenbeckstr. 1, 55101 Mainz, GermanyKing Faisal Specialist Hospital & Research Centre, Children's Cancer Centre, PO Box 3354, MBC # 98-16, Riyadh 11211, Saudi ArabiaLaboratory of Pathology, Gene Regulation Section, National Cancer Institute, NIH, Bethesda 20892, MD, USABackground: Chromosomal translocations are causally related to the development of many tumors. In Burkitt's lymphoma, abnormalities involving the c-myc gene are essential. The CT-element of the c-myc promoter adopts non-B-conformation in vivo and in vitro, and therefore provides a potential fragile site. Methods: We have developed a LM-PCR-based approach to test if chromosomal breakpoints indeed cluster in this region. Results: Amplifying both, wild-type as well as the translocated c-myc gene by LM-PCR, it was shown that chromosomal breakpoints did not cluster within the CT-element. Conclusions: Therefore, the CT-element is not especially susceptible to the formation of breakpoints leading to chromosomal translocations in Burkitt's lymphoma.http://dx.doi.org/10.1155/2006/695262 |
| spellingShingle | Achim Weber Marina I. Gutierrez David Levens The CT-Element of The C-Myc Gene Does Not Predispose to Chromosomal Breakpoints in Burkitt'S Lymphoma Cellular Oncology |
| title | The CT-Element of The C-Myc Gene Does Not Predispose to Chromosomal Breakpoints in Burkitt'S Lymphoma |
| title_full | The CT-Element of The C-Myc Gene Does Not Predispose to Chromosomal Breakpoints in Burkitt'S Lymphoma |
| title_fullStr | The CT-Element of The C-Myc Gene Does Not Predispose to Chromosomal Breakpoints in Burkitt'S Lymphoma |
| title_full_unstemmed | The CT-Element of The C-Myc Gene Does Not Predispose to Chromosomal Breakpoints in Burkitt'S Lymphoma |
| title_short | The CT-Element of The C-Myc Gene Does Not Predispose to Chromosomal Breakpoints in Burkitt'S Lymphoma |
| title_sort | ct element of the c myc gene does not predispose to chromosomal breakpoints in burkitt s lymphoma |
| url | http://dx.doi.org/10.1155/2006/695262 |
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