Effect of Light Spectrum, Sucrose Concentration, and 6-Benzyl-aminopurine on In Vitro Adventitious Bulb Formation in <i>Tulipa tarda</i>
This study presents the optimal light spectrum and medium composition for the in vitro induction of adventitious bulbs in <i>T. tarda</i> Stapf. Bulb scales, used as explants, were cultivated on 100% MS solid media with 3% or 6% sucrose and 0 or 0.5 µM BAP (6-benzyl-aminopurine) under a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-03-01
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| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/15/3/642 |
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| Summary: | This study presents the optimal light spectrum and medium composition for the in vitro induction of adventitious bulbs in <i>T. tarda</i> Stapf. Bulb scales, used as explants, were cultivated on 100% MS solid media with 3% or 6% sucrose and 0 or 0.5 µM BAP (6-benzyl-aminopurine) under a 16 h photoperiod and different fluorescent light treatments (white, red, blue). Darkness was used as a control. The cultures were maintained at 20 ± 2 °C for 12 weeks. The obtained results revealed that white light combined with 6% sucrose yielded the highest adventitious bulb formation, with an average of 12.1 ± 1.3 bulbs per explant. The bulbs formed directly on the surface of the explants. Red light combined with 3% sucrose and 0.5 µM BAP completely inhibited bulb formation, while darkness promoted leaf development, with a maximum of 1.3 ± 0.1 leaves per bulb, under darkness on medium with 6% sucrose. The addition of BAP, in most cases, was essential for root formation, with a maximum of 2.9 ± 0.6 roots per bulb under the influence of white light. This study demonstrates that it is possible to obtain an effective and efficient method for <i>T. tarda</i> propagation from bulb scales treated with 6% sucrose under white light condition. This method offers the potential for the commercial cultivation of this ornamental species. |
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| ISSN: | 2073-4395 |