Mitochondrial COI Haplotype Diversity of Rhynchobatus australiae Collected from Ketapang Fish Port, Bangka Belitung Islands
Rhynchobatus australiae is a member of the Rhinidae family and can be found in the Indo Pacific. This species is categorized as Critically Endangered according to The International Union for Conservation of Nature and Natural Resources (IUCN) Red List and listed as Appendix II Convention on Internat...
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Diponegoro University; Association of Indonesian Coastal Management Experts
2022-06-01
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| Series: | Ilmu Kelautan |
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| Online Access: | https://ejournal.undip.ac.id/index.php/ijms/article/view/36075 |
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| author | Novita Permata Putri Irwani Irwani Munasik Munasik Ranny Ramadhani Yuneni Faqih Akbar Alghozali Angka Mahardini |
| author_facet | Novita Permata Putri Irwani Irwani Munasik Munasik Ranny Ramadhani Yuneni Faqih Akbar Alghozali Angka Mahardini |
| author_sort | Novita Permata Putri |
| collection | DOAJ |
| description | Rhynchobatus australiae is a member of the Rhinidae family and can be found in the Indo Pacific. This species is categorized as Critically Endangered according to The International Union for Conservation of Nature and Natural Resources (IUCN) Red List and listed as Appendix II Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES), due to population declines. Sustainable fisheries management is urgently needed. Information related to genetic diversity is one of the most important aspects of information for appropriate sustainable fisheries management. Therefore, this research aims to investigate the genetic diversity of Rhynchobatus australiae collected from Ketapang Fish Port Bangka Belitung Islands. Total 21 samples were collected at Ketapang Fish Port. DNA extraction was carried out using the 10% chelex method and amplification was done through PCR method on the mitochondrial DNA using Fish BCL and Fish BCH primer. Sequences in size of 653 base pairs were successfully obtained from all fish samples showing the existence of 2 clades from the construction of the phylogeny tree with close genetic distance. Results showed high haplotype diversity (Hd: 0.733 ± 0.082) and low nucleotide diversity (π: 0.00176± 0.00036) with 6 polymorphic sites (S) from 7 unique haplotypes (h). This research provides basic information of genetic diversity of Rhynchobatus australiae collected from Ketapang Fish Port and complements other information to better understand the status of the threatened Rhynchobatus australiae population. |
| format | Article |
| id | doaj-art-1b38b957d84d4a61aac3d2f0a30d0230 |
| institution | DOAJ |
| issn | 0853-7291 2406-7598 |
| language | English |
| publishDate | 2022-06-01 |
| publisher | Diponegoro University; Association of Indonesian Coastal Management Experts |
| record_format | Article |
| series | Ilmu Kelautan |
| spelling | doaj-art-1b38b957d84d4a61aac3d2f0a30d02302025-08-20T02:43:50ZengDiponegoro University; Association of Indonesian Coastal Management ExpertsIlmu Kelautan0853-72912406-75982022-06-0127213314010.14710/ik.ijms.27.2.133-14020588Mitochondrial COI Haplotype Diversity of Rhynchobatus australiae Collected from Ketapang Fish Port, Bangka Belitung IslandsNovita Permata Putri0Irwani Irwani1Munasik Munasik2Ranny Ramadhani Yuneni3Faqih Akbar Alghozali4Angka Mahardini5Department Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro, IndonesiaDepartment Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro, IndonesiaDepartment Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro, IndonesiaWWF Indonesia, IndonesiaWWF Indonesia, Indonesia3Jeju Marine Research Center, Korea Institute of Ocean Science and Technology, IndonesiaRhynchobatus australiae is a member of the Rhinidae family and can be found in the Indo Pacific. This species is categorized as Critically Endangered according to The International Union for Conservation of Nature and Natural Resources (IUCN) Red List and listed as Appendix II Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES), due to population declines. Sustainable fisheries management is urgently needed. Information related to genetic diversity is one of the most important aspects of information for appropriate sustainable fisheries management. Therefore, this research aims to investigate the genetic diversity of Rhynchobatus australiae collected from Ketapang Fish Port Bangka Belitung Islands. Total 21 samples were collected at Ketapang Fish Port. DNA extraction was carried out using the 10% chelex method and amplification was done through PCR method on the mitochondrial DNA using Fish BCL and Fish BCH primer. Sequences in size of 653 base pairs were successfully obtained from all fish samples showing the existence of 2 clades from the construction of the phylogeny tree with close genetic distance. Results showed high haplotype diversity (Hd: 0.733 ± 0.082) and low nucleotide diversity (π: 0.00176± 0.00036) with 6 polymorphic sites (S) from 7 unique haplotypes (h). This research provides basic information of genetic diversity of Rhynchobatus australiae collected from Ketapang Fish Port and complements other information to better understand the status of the threatened Rhynchobatus australiae population.https://ejournal.undip.ac.id/index.php/ijms/article/view/36075genetic diversitywedgefishrhynchobatus australiaebangka belitung islands |
| spellingShingle | Novita Permata Putri Irwani Irwani Munasik Munasik Ranny Ramadhani Yuneni Faqih Akbar Alghozali Angka Mahardini Mitochondrial COI Haplotype Diversity of Rhynchobatus australiae Collected from Ketapang Fish Port, Bangka Belitung Islands Ilmu Kelautan genetic diversity wedgefish rhynchobatus australiae bangka belitung islands |
| title | Mitochondrial COI Haplotype Diversity of Rhynchobatus australiae Collected from Ketapang Fish Port, Bangka Belitung Islands |
| title_full | Mitochondrial COI Haplotype Diversity of Rhynchobatus australiae Collected from Ketapang Fish Port, Bangka Belitung Islands |
| title_fullStr | Mitochondrial COI Haplotype Diversity of Rhynchobatus australiae Collected from Ketapang Fish Port, Bangka Belitung Islands |
| title_full_unstemmed | Mitochondrial COI Haplotype Diversity of Rhynchobatus australiae Collected from Ketapang Fish Port, Bangka Belitung Islands |
| title_short | Mitochondrial COI Haplotype Diversity of Rhynchobatus australiae Collected from Ketapang Fish Port, Bangka Belitung Islands |
| title_sort | mitochondrial coi haplotype diversity of rhynchobatus australiae collected from ketapang fish port bangka belitung islands |
| topic | genetic diversity wedgefish rhynchobatus australiae bangka belitung islands |
| url | https://ejournal.undip.ac.id/index.php/ijms/article/view/36075 |
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