Evolution and biological characterization of H5N1 influenza viruses bearing the clade 2.3.2.1 hemagglutinin gene
H5N1 avian influenza viruses bearing the clade 2.3.2.1 hemagglutinin (HA) gene have been widely detected in birds and poultry in several countries. During our routine surveillance, we isolated 28 H5N1 viruses between January 2017 and October 2020. To investigate the genetic relationship of the globa...
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| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2023.2284294 |
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| _version_ | 1850259671669014528 |
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| author | Xin Xing Jianzhong Shi Pengfei Cui Cheng Yan Yaping Zhang Yuancheng Zhang Congcong Wang Yuan Chen Xianying Zeng Guobin Tian Liling Liu Yuntao Guan Chengjun Li Yasuo Suzuki Guohua Deng Hualan Chen |
| author_facet | Xin Xing Jianzhong Shi Pengfei Cui Cheng Yan Yaping Zhang Yuancheng Zhang Congcong Wang Yuan Chen Xianying Zeng Guobin Tian Liling Liu Yuntao Guan Chengjun Li Yasuo Suzuki Guohua Deng Hualan Chen |
| author_sort | Xin Xing |
| collection | DOAJ |
| description | H5N1 avian influenza viruses bearing the clade 2.3.2.1 hemagglutinin (HA) gene have been widely detected in birds and poultry in several countries. During our routine surveillance, we isolated 28 H5N1 viruses between January 2017 and October 2020. To investigate the genetic relationship of the globally circulating H5N1 viruses and the biological properties of those detected in China, we performed a detailed phylogenic analysis of 274 representative H5N1 strains and analyzed the antigenic properties, receptor-binding preference, and virulence in mice of the H5N1 viruses isolated in China. The phylogenic analysis indicated that the HA genes of the 274 viruses belonged to six subclades, namely clades 2.3.2.1a to 2.3.2.1f; these viruses acquired gene mutations and underwent complicated reassortment to form 58 genotypes, with G43 being the dominant genotype detected in eight Asian and African countries. The 28 H5N1 viruses detected in this study carried the HA of clade 2.3.2.1c (two strains), 2.3.2.1d (three strains), or 2.3.2.1f (23 strains), and formed eight genotypes. These viruses were antigenically well-matched with the H5-Re12 vaccine strain used in China. Animal studies showed that the pathogenicity of the H5N1 viruses ranged from non-lethal to highly lethal in mice. Moreover, the viruses exclusively bound to avian-type receptors and have not acquired the ability to bind to human-type receptors. Our study reveals the overall picture of the evolution of clade 2.3.2.1 H5N1 viruses and provides insights into the control of these viruses. |
| format | Article |
| id | doaj-art-1b28ce17fbeb4bfa9c73bc2a04d5f794 |
| institution | OA Journals |
| issn | 2222-1751 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-1b28ce17fbeb4bfa9c73bc2a04d5f7942025-08-20T01:55:49ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512024-12-0113110.1080/22221751.2023.2284294Evolution and biological characterization of H5N1 influenza viruses bearing the clade 2.3.2.1 hemagglutinin geneXin Xing0Jianzhong Shi1Pengfei Cui2Cheng Yan3Yaping Zhang4Yuancheng Zhang5Congcong Wang6Yuan Chen7Xianying Zeng8Guobin Tian9Liling Liu10Yuntao Guan11Chengjun Li12Yasuo Suzuki13Guohua Deng14Hualan Chen15State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of ChinaState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of ChinaState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of ChinaState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of ChinaState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of ChinaState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of ChinaState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of ChinaState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of ChinaState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of ChinaState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of ChinaState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of ChinaState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of ChinaState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of ChinaDepartment of Medical Biochemistry, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka, JapanState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of ChinaState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of ChinaH5N1 avian influenza viruses bearing the clade 2.3.2.1 hemagglutinin (HA) gene have been widely detected in birds and poultry in several countries. During our routine surveillance, we isolated 28 H5N1 viruses between January 2017 and October 2020. To investigate the genetic relationship of the globally circulating H5N1 viruses and the biological properties of those detected in China, we performed a detailed phylogenic analysis of 274 representative H5N1 strains and analyzed the antigenic properties, receptor-binding preference, and virulence in mice of the H5N1 viruses isolated in China. The phylogenic analysis indicated that the HA genes of the 274 viruses belonged to six subclades, namely clades 2.3.2.1a to 2.3.2.1f; these viruses acquired gene mutations and underwent complicated reassortment to form 58 genotypes, with G43 being the dominant genotype detected in eight Asian and African countries. The 28 H5N1 viruses detected in this study carried the HA of clade 2.3.2.1c (two strains), 2.3.2.1d (three strains), or 2.3.2.1f (23 strains), and formed eight genotypes. These viruses were antigenically well-matched with the H5-Re12 vaccine strain used in China. Animal studies showed that the pathogenicity of the H5N1 viruses ranged from non-lethal to highly lethal in mice. Moreover, the viruses exclusively bound to avian-type receptors and have not acquired the ability to bind to human-type receptors. Our study reveals the overall picture of the evolution of clade 2.3.2.1 H5N1 viruses and provides insights into the control of these viruses.https://www.tandfonline.com/doi/10.1080/22221751.2023.2284294Avian influenza virusH5N1clade 2.3.2.1evolutionpathogenicityantigenicity |
| spellingShingle | Xin Xing Jianzhong Shi Pengfei Cui Cheng Yan Yaping Zhang Yuancheng Zhang Congcong Wang Yuan Chen Xianying Zeng Guobin Tian Liling Liu Yuntao Guan Chengjun Li Yasuo Suzuki Guohua Deng Hualan Chen Evolution and biological characterization of H5N1 influenza viruses bearing the clade 2.3.2.1 hemagglutinin gene Emerging Microbes and Infections Avian influenza virus H5N1 clade 2.3.2.1 evolution pathogenicity antigenicity |
| title | Evolution and biological characterization of H5N1 influenza viruses bearing the clade 2.3.2.1 hemagglutinin gene |
| title_full | Evolution and biological characterization of H5N1 influenza viruses bearing the clade 2.3.2.1 hemagglutinin gene |
| title_fullStr | Evolution and biological characterization of H5N1 influenza viruses bearing the clade 2.3.2.1 hemagglutinin gene |
| title_full_unstemmed | Evolution and biological characterization of H5N1 influenza viruses bearing the clade 2.3.2.1 hemagglutinin gene |
| title_short | Evolution and biological characterization of H5N1 influenza viruses bearing the clade 2.3.2.1 hemagglutinin gene |
| title_sort | evolution and biological characterization of h5n1 influenza viruses bearing the clade 2 3 2 1 hemagglutinin gene |
| topic | Avian influenza virus H5N1 clade 2.3.2.1 evolution pathogenicity antigenicity |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2023.2284294 |
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