The Effect of Mono- and Di-Saccharides on the Microbiome of Dairy Cow Manure and Its Odor
In a previous experiment, we showed that the odor of <i>Bos taurus</i> manure slurries could be improved by anaerobic incubation with the sugars glucose, lactose, and sucrose. This improvement was due to reductions in the concentrations of malodorants, including dimethyl disulfide, <i...
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2024-12-01
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| author | John H. Loughrin Getahun E. Agga |
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| description | In a previous experiment, we showed that the odor of <i>Bos taurus</i> manure slurries could be improved by anaerobic incubation with the sugars glucose, lactose, and sucrose. This improvement was due to reductions in the concentrations of malodorants, including dimethyl disulfide, <i>p</i>-cresol, <i>p</i>-ethylphenol, indole, and skatole, and a shift to the production of fruity esters, including ethyl butyrate and propyl propanoate. Due to large concentrations of lactic acid produced by the sugar-amended manure slurries, we inferred that lactic acid bacteria were involved in improving the manure slurry odor. Here, through 16S rRNA amplicon sequencing for microbiome analysis, we show that lactic acid bacterial growth was promoted by the addition of all three sugars. <i>Lactobacillus buchneri</i> and an unknown <i>Lactobacillus</i> sp. were the most prominent lactic acid bacteria stimulated by sugar addition. Lactobacillales were found only in trace abundances in unamended manure slurries. The relative abundance of orders such as Clostridiales, Bifidobacteriales, and Erysipelotrichales were not noticeably affected by sugar amendment. However, the disaccharides lactose and sucrose seemed to increase the relative abundance of <i>Bifidobacterium,</i> whereas the monosaccharide glucose did not. We conclude that lactic acid bacteria are the primary bacteria involved in improving odor in dairy cow manure slurries and present strategies to enhance their abundance in animal wastes. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-1af282c218ea492b8dded16b0ad2a90d2025-01-24T13:42:26ZengMDPI AGMicroorganisms2076-26072024-12-011315210.3390/microorganisms13010052The Effect of Mono- and Di-Saccharides on the Microbiome of Dairy Cow Manure and Its OdorJohn H. Loughrin0Getahun E. Agga1Food Animal Environmental Systems Research Unit, Agricultural Research Service, United States Department of Agriculture, 2413 Nashville Road, Suite B5, Bowling Green, KY 42101, USAFood Animal Environmental Systems Research Unit, Agricultural Research Service, United States Department of Agriculture, 2413 Nashville Road, Suite B5, Bowling Green, KY 42101, USAIn a previous experiment, we showed that the odor of <i>Bos taurus</i> manure slurries could be improved by anaerobic incubation with the sugars glucose, lactose, and sucrose. This improvement was due to reductions in the concentrations of malodorants, including dimethyl disulfide, <i>p</i>-cresol, <i>p</i>-ethylphenol, indole, and skatole, and a shift to the production of fruity esters, including ethyl butyrate and propyl propanoate. Due to large concentrations of lactic acid produced by the sugar-amended manure slurries, we inferred that lactic acid bacteria were involved in improving the manure slurry odor. Here, through 16S rRNA amplicon sequencing for microbiome analysis, we show that lactic acid bacterial growth was promoted by the addition of all three sugars. <i>Lactobacillus buchneri</i> and an unknown <i>Lactobacillus</i> sp. were the most prominent lactic acid bacteria stimulated by sugar addition. Lactobacillales were found only in trace abundances in unamended manure slurries. The relative abundance of orders such as Clostridiales, Bifidobacteriales, and Erysipelotrichales were not noticeably affected by sugar amendment. However, the disaccharides lactose and sucrose seemed to increase the relative abundance of <i>Bifidobacterium,</i> whereas the monosaccharide glucose did not. We conclude that lactic acid bacteria are the primary bacteria involved in improving odor in dairy cow manure slurries and present strategies to enhance their abundance in animal wastes.https://www.mdpi.com/2076-2607/13/1/5216S rRNAamplicon sequencinganimal manuredimethyl disulfidelactic acid bacteriamalodor |
| spellingShingle | John H. Loughrin Getahun E. Agga The Effect of Mono- and Di-Saccharides on the Microbiome of Dairy Cow Manure and Its Odor Microorganisms 16S rRNA amplicon sequencing animal manure dimethyl disulfide lactic acid bacteria malodor |
| title | The Effect of Mono- and Di-Saccharides on the Microbiome of Dairy Cow Manure and Its Odor |
| title_full | The Effect of Mono- and Di-Saccharides on the Microbiome of Dairy Cow Manure and Its Odor |
| title_fullStr | The Effect of Mono- and Di-Saccharides on the Microbiome of Dairy Cow Manure and Its Odor |
| title_full_unstemmed | The Effect of Mono- and Di-Saccharides on the Microbiome of Dairy Cow Manure and Its Odor |
| title_short | The Effect of Mono- and Di-Saccharides on the Microbiome of Dairy Cow Manure and Its Odor |
| title_sort | effect of mono and di saccharides on the microbiome of dairy cow manure and its odor |
| topic | 16S rRNA amplicon sequencing animal manure dimethyl disulfide lactic acid bacteria malodor |
| url | https://www.mdpi.com/2076-2607/13/1/52 |
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