Sequence Analysis of the UCP1 Gene in a Severe Obese Population from Southern Italy
Brown adipose tissue, where Uncoupling Protein 1 (UCP1) activity uncouples mitochondrial respiration, is an important site of facultative energy expenditure. This tissue may normally function to prevent obesity. Our aim was to investigate by sequence analysis the presence of UCP1 gene variations th...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Journal of Obesity |
| Online Access: | http://dx.doi.org/10.1155/2011/269043 |
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| author | Giuseppe Labruna Fabrizio Pasanisi Giuliana Fortunato Carmela Nardelli Carmine Finelli Eduardo Farinaro Franco Contaldo Lucia Sacchetti |
| author_facet | Giuseppe Labruna Fabrizio Pasanisi Giuliana Fortunato Carmela Nardelli Carmine Finelli Eduardo Farinaro Franco Contaldo Lucia Sacchetti |
| author_sort | Giuseppe Labruna |
| collection | DOAJ |
| description | Brown adipose tissue, where Uncoupling Protein 1 (UCP1) activity uncouples mitochondrial respiration, is an important site of facultative energy expenditure. This tissue may normally function to prevent obesity. Our aim was to investigate by sequence analysis the presence of UCP1 gene variations that may be associated with obesity. We studied 100 severe obese adults (BMI > 40 kg/m2) and 100 normal-weight control subjects (BMI range = 19–24.9 kg/m2). We identified 7 variations in the promoter region, 4 in the intronic region and 4 in the exonic region. Globally, 72% of obese patients bore UCP1 polymorphisms. Among UCP1 variants, g.IVS4−208T>G SNP was associated with obesity (OR: 1.77; 95% CI = 1.26–2.50; P=.001). Further, obese patients bearing the g.−451C>T (CT+TT) or the g.940G>A (GA+AA) genotypes showed a higher BMI than not polymorphic obese patients (P=.008 and P=.043, resp.). In conclusion, UCP1 SNPs could represent “thrifty” factors that promote energy storage in prone subjects. |
| format | Article |
| id | doaj-art-1a80a5e768174d318519213c0cac38f0 |
| institution | Kabale University |
| issn | 2090-0708 2090-0716 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Obesity |
| spelling | doaj-art-1a80a5e768174d318519213c0cac38f02025-02-03T01:22:29ZengWileyJournal of Obesity2090-07082090-07162011-01-01201110.1155/2011/269043269043Sequence Analysis of the UCP1 Gene in a Severe Obese Population from Southern ItalyGiuseppe Labruna0Fabrizio Pasanisi1Giuliana Fortunato2Carmela Nardelli3Carmine Finelli4Eduardo Farinaro5Franco Contaldo6Lucia Sacchetti7Fondazione IRCCS SDN, Istituto di Ricerca Diagnostica e Nucleare, Via Gianturco 113, 80143 Naples, ItalyCentro Interuniversitario di Studi e Ricerche sull'Obesità e Dipartimento di Medicina Clinica e Sperimentale, Università degli Studi di Napoli Federico II, Via Pansini 5, 80131 Naples, ItalyCEINGE Biotecnologie Avanzate S.C. a R.L., Via Gaetano Salvatore 486, 80145, Naples, ItalyCEINGE Biotecnologie Avanzate S.C. a R.L., Via Gaetano Salvatore 486, 80145, Naples, ItalyFondazione Stella Maris Mediterraneo, Centro Disturbi del Comportamento Alimentare e del Peso “G. Gioia”, Chiaromonte, C/da S. Lucia, 85100, Chiaromonte, Potenza, ItalyDipartimento di Scienze Mediche Preventive, Università degli Studi di Napoli Federico II, Via Pansini 5, 80131 Naples, ItalyCentro Interuniversitario di Studi e Ricerche sull'Obesità e Dipartimento di Medicina Clinica e Sperimentale, Università degli Studi di Napoli Federico II, Via Pansini 5, 80131 Naples, ItalyCEINGE Biotecnologie Avanzate S.C. a R.L., Via Gaetano Salvatore 486, 80145, Naples, ItalyBrown adipose tissue, where Uncoupling Protein 1 (UCP1) activity uncouples mitochondrial respiration, is an important site of facultative energy expenditure. This tissue may normally function to prevent obesity. Our aim was to investigate by sequence analysis the presence of UCP1 gene variations that may be associated with obesity. We studied 100 severe obese adults (BMI > 40 kg/m2) and 100 normal-weight control subjects (BMI range = 19–24.9 kg/m2). We identified 7 variations in the promoter region, 4 in the intronic region and 4 in the exonic region. Globally, 72% of obese patients bore UCP1 polymorphisms. Among UCP1 variants, g.IVS4−208T>G SNP was associated with obesity (OR: 1.77; 95% CI = 1.26–2.50; P=.001). Further, obese patients bearing the g.−451C>T (CT+TT) or the g.940G>A (GA+AA) genotypes showed a higher BMI than not polymorphic obese patients (P=.008 and P=.043, resp.). In conclusion, UCP1 SNPs could represent “thrifty” factors that promote energy storage in prone subjects.http://dx.doi.org/10.1155/2011/269043 |
| spellingShingle | Giuseppe Labruna Fabrizio Pasanisi Giuliana Fortunato Carmela Nardelli Carmine Finelli Eduardo Farinaro Franco Contaldo Lucia Sacchetti Sequence Analysis of the UCP1 Gene in a Severe Obese Population from Southern Italy Journal of Obesity |
| title | Sequence Analysis of the UCP1 Gene in a Severe Obese Population from Southern Italy |
| title_full | Sequence Analysis of the UCP1 Gene in a Severe Obese Population from Southern Italy |
| title_fullStr | Sequence Analysis of the UCP1 Gene in a Severe Obese Population from Southern Italy |
| title_full_unstemmed | Sequence Analysis of the UCP1 Gene in a Severe Obese Population from Southern Italy |
| title_short | Sequence Analysis of the UCP1 Gene in a Severe Obese Population from Southern Italy |
| title_sort | sequence analysis of the ucp1 gene in a severe obese population from southern italy |
| url | http://dx.doi.org/10.1155/2011/269043 |
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