Participation of gut microbiota and bacterial translocation in chronic systemic inflammation in recently diagnosed rheumatoid arthritis patients
The objective of this study was to investigate the link between gut microbiota (GM) dysbiosis, gut inflammation, and bacterial translocation (BT) in recently diagnosed rheumatoid arthritis (RA). This case-control, observational study prospectively recruited recently diagnosed (<12 months) RA pati...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Current Research in Microbial Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666517425000288 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849330796808110080 |
|---|---|
| author | Catherine Dunyach-Remy Cassandra Pouget Yves-Marie Pers Cécile Gaujoux-Viala Christophe Demattei Florian Salipante Lucia Grenga Jean Armengaud Jean-Philippe Lavigne Christian Jorgensen |
| author_facet | Catherine Dunyach-Remy Cassandra Pouget Yves-Marie Pers Cécile Gaujoux-Viala Christophe Demattei Florian Salipante Lucia Grenga Jean Armengaud Jean-Philippe Lavigne Christian Jorgensen |
| author_sort | Catherine Dunyach-Remy |
| collection | DOAJ |
| description | The objective of this study was to investigate the link between gut microbiota (GM) dysbiosis, gut inflammation, and bacterial translocation (BT) in recently diagnosed rheumatoid arthritis (RA). This case-control, observational study prospectively recruited recently diagnosed (<12 months) RA patients and age-matched healthy controls (HC) from two French hospitals between July 2014 to March 2018. The primary objective was to investigate GM composition in each group using 16S rRNA sequencing and metaproteomics approaches. Three plasmatic BT markers (sCD14, LPS-binding protein, and number of 16S rRNA gene copies) and one intestinal permeability marker (I-FABP) were quantified in blood samples.Twenty-five were included in each group, and 50 stools and blood samples were analyzed. 16S rRNA gene analysis showed an decrease in Coprococcus in RA patients after Body Mass Index and HLA status. Circulating bacterial DNA (number of copies of the 16S rRNA gene) and plasmatic I-FABP were higher in RA patients compared to HCs (p < 0.01), indicating increased BT and intestinal permeability in these patients. Metaproteomics from stool samples highlighted an increased host humoral immune response in RA, with elevated levels of inflammatory proteins (azurocidin, cathepsin G, neutrophil defensing 1). Gut inflammation may contribute to increased intestinal permeability, leading to BT into the systemic circulation and thus chronic inflammation. |
| format | Article |
| id | doaj-art-1a7fbaa4d2964116a7ca3f663ed439f5 |
| institution | Kabale University |
| issn | 2666-5174 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Microbial Sciences |
| spelling | doaj-art-1a7fbaa4d2964116a7ca3f663ed439f52025-08-20T03:46:49ZengElsevierCurrent Research in Microbial Sciences2666-51742025-01-01810036610.1016/j.crmicr.2025.100366Participation of gut microbiota and bacterial translocation in chronic systemic inflammation in recently diagnosed rheumatoid arthritis patientsCatherine Dunyach-Remy0Cassandra Pouget1Yves-Marie Pers2Cécile Gaujoux-Viala3Christophe Demattei4Florian Salipante5Lucia Grenga6Jean Armengaud7Jean-Philippe Lavigne8Christian Jorgensen9Bacterial Virulence and Chronic Infections, INSERM U1047, Univ Montpellier, Department of Microbiology and Hospital Hygiene, CHU Nîmes, Nîmes, France; Correspondence author: Univeristy Hospital of Nîmes, place Robert Debré, 30 908 Nîmes, FranceBacterial Virulence and Chronic Infections, INSERM U1047, Univ Montpellier, Department of Microbiology and Hospital Hygiene, CHU Nîmes, Nîmes, FranceDepartment of Rheumatology, Stem cells, Cellular plasticity, Regenerative medicine and Immunotherapies, IRMB, INSERM UMR1183, University of Montpellier & University Hospital of Montpellier, Montpellier, FranceDesbrest Institute of Epidemiology and Public Health, University of Montpellier, INSERM, Department of Rheumatology, CHU Nîmes, Montpellier, FranceDepartment of Biostatistics, Epidemiology, Public Health and Innovation in Methodology (BESPIM), CHU Nîmes, Univ Montpellier, Nîmes, FranceDepartment of Biostatistics, Epidemiology, Public Health and Innovation in Methodology (BESPIM), CHU Nîmes, Univ Montpellier, Nîmes, FranceDepartment of Medicines and Technologies for Health, Atomic Energy and Alternative Energies Commission (CEA), Paris-Saclay University, Bagnols-sur-Cèze, FranceDepartment of Medicines and Technologies for Health, Atomic Energy and Alternative Energies Commission (CEA), Paris-Saclay University, Bagnols-sur-Cèze, FranceBacterial Virulence and Chronic Infections, INSERM U1047, Univ Montpellier, Department of Microbiology and Hospital Hygiene, CHU Nîmes, Nîmes, FranceDepartment of Rheumatology, Stem cells, Cellular plasticity, Regenerative medicine and Immunotherapies, IRMB, INSERM UMR1183, University of Montpellier & University Hospital of Montpellier, Montpellier, FranceThe objective of this study was to investigate the link between gut microbiota (GM) dysbiosis, gut inflammation, and bacterial translocation (BT) in recently diagnosed rheumatoid arthritis (RA). This case-control, observational study prospectively recruited recently diagnosed (<12 months) RA patients and age-matched healthy controls (HC) from two French hospitals between July 2014 to March 2018. The primary objective was to investigate GM composition in each group using 16S rRNA sequencing and metaproteomics approaches. Three plasmatic BT markers (sCD14, LPS-binding protein, and number of 16S rRNA gene copies) and one intestinal permeability marker (I-FABP) were quantified in blood samples.Twenty-five were included in each group, and 50 stools and blood samples were analyzed. 16S rRNA gene analysis showed an decrease in Coprococcus in RA patients after Body Mass Index and HLA status. Circulating bacterial DNA (number of copies of the 16S rRNA gene) and plasmatic I-FABP were higher in RA patients compared to HCs (p < 0.01), indicating increased BT and intestinal permeability in these patients. Metaproteomics from stool samples highlighted an increased host humoral immune response in RA, with elevated levels of inflammatory proteins (azurocidin, cathepsin G, neutrophil defensing 1). Gut inflammation may contribute to increased intestinal permeability, leading to BT into the systemic circulation and thus chronic inflammation.http://www.sciencedirect.com/science/article/pii/S2666517425000288Rheumatoid arthritisGut microbiotaBacterial translocationIntestinal permeability |
| spellingShingle | Catherine Dunyach-Remy Cassandra Pouget Yves-Marie Pers Cécile Gaujoux-Viala Christophe Demattei Florian Salipante Lucia Grenga Jean Armengaud Jean-Philippe Lavigne Christian Jorgensen Participation of gut microbiota and bacterial translocation in chronic systemic inflammation in recently diagnosed rheumatoid arthritis patients Current Research in Microbial Sciences Rheumatoid arthritis Gut microbiota Bacterial translocation Intestinal permeability |
| title | Participation of gut microbiota and bacterial translocation in chronic systemic inflammation in recently diagnosed rheumatoid arthritis patients |
| title_full | Participation of gut microbiota and bacterial translocation in chronic systemic inflammation in recently diagnosed rheumatoid arthritis patients |
| title_fullStr | Participation of gut microbiota and bacterial translocation in chronic systemic inflammation in recently diagnosed rheumatoid arthritis patients |
| title_full_unstemmed | Participation of gut microbiota and bacterial translocation in chronic systemic inflammation in recently diagnosed rheumatoid arthritis patients |
| title_short | Participation of gut microbiota and bacterial translocation in chronic systemic inflammation in recently diagnosed rheumatoid arthritis patients |
| title_sort | participation of gut microbiota and bacterial translocation in chronic systemic inflammation in recently diagnosed rheumatoid arthritis patients |
| topic | Rheumatoid arthritis Gut microbiota Bacterial translocation Intestinal permeability |
| url | http://www.sciencedirect.com/science/article/pii/S2666517425000288 |
| work_keys_str_mv | AT catherinedunyachremy participationofgutmicrobiotaandbacterialtranslocationinchronicsystemicinflammationinrecentlydiagnosedrheumatoidarthritispatients AT cassandrapouget participationofgutmicrobiotaandbacterialtranslocationinchronicsystemicinflammationinrecentlydiagnosedrheumatoidarthritispatients AT yvesmariepers participationofgutmicrobiotaandbacterialtranslocationinchronicsystemicinflammationinrecentlydiagnosedrheumatoidarthritispatients AT cecilegaujouxviala participationofgutmicrobiotaandbacterialtranslocationinchronicsystemicinflammationinrecentlydiagnosedrheumatoidarthritispatients AT christophedemattei participationofgutmicrobiotaandbacterialtranslocationinchronicsystemicinflammationinrecentlydiagnosedrheumatoidarthritispatients AT floriansalipante participationofgutmicrobiotaandbacterialtranslocationinchronicsystemicinflammationinrecentlydiagnosedrheumatoidarthritispatients AT luciagrenga participationofgutmicrobiotaandbacterialtranslocationinchronicsystemicinflammationinrecentlydiagnosedrheumatoidarthritispatients AT jeanarmengaud participationofgutmicrobiotaandbacterialtranslocationinchronicsystemicinflammationinrecentlydiagnosedrheumatoidarthritispatients AT jeanphilippelavigne participationofgutmicrobiotaandbacterialtranslocationinchronicsystemicinflammationinrecentlydiagnosedrheumatoidarthritispatients AT christianjorgensen participationofgutmicrobiotaandbacterialtranslocationinchronicsystemicinflammationinrecentlydiagnosedrheumatoidarthritispatients |