NIPAL1 as a prognostic biomarker associated with pancreatic adenocarcinoma progression and immune infiltration
Abstract Pancreatic adenocarcinoma (PAAD) is a highly malignant tumor in the digestive system, with an increasing incidence and mortality rate globally. Recent genetic studies have revealed that the abnormal expression and functional dysregulation of various genes are involved in the occurrence and...
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BMC
2025-01-01
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| Online Access: | https://doi.org/10.1186/s12885-025-13567-5 |
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| author | Youlong Zhu Zongze Qi Shaoqi Zu Fangchao Yang Yanming Wang Lei Zhu Xintong Li Ruixue Li Hong Zhu |
| author_facet | Youlong Zhu Zongze Qi Shaoqi Zu Fangchao Yang Yanming Wang Lei Zhu Xintong Li Ruixue Li Hong Zhu |
| author_sort | Youlong Zhu |
| collection | DOAJ |
| description | Abstract Pancreatic adenocarcinoma (PAAD) is a highly malignant tumor in the digestive system, with an increasing incidence and mortality rate globally. Recent genetic studies have revealed that the abnormal expression and functional dysregulation of various genes are involved in the occurrence and progression of pancreatic cancer. NIPA-like proteins (NIPAs) are expressed in a variety of cancer types, yet the role of NIPAL1 in cancer remains unclear. Therefore, further research is required to determine its diagnostic significance and understand its biological functions in cancer. Primitive RNA sequencing (RNA-seq) data of PAAD from The Cancer Genome Atlas (TCGA) was utilized for bioinformatics analysis to characterize the expression levels of NIPAL1 in tumor and normal tissues. Differentially expressed genes (DEGs) were identified, and Gene Set Enrichment Analysis (GSEA) was performed to elucidate potential biological mechanisms of NIPAL1 involved in PAAD development. Additionally, we analyzed the correlation between NIPAL1 expression, immune cell infiltration, and PAAD progression. The Genomics of Drug Sensitivity in Cancer (GDSC) database was utilized to investigate the relationship between NIPAL1 expression and the efficacy of common drugs used in chemotherapy and targeted therapy in patients with pancreatic cancer. Subsequently, we predicted five small-molecule drugs targeted at NIPAL1 using molecular docking. Finally, high expression of NIPAL1 in tumor tissues was validated through immunohistochemistry. In pancreatic cancer cell lines, changes in phenotypes such as proliferation, migration, and invasion following the knockdown of NIPAL1 were assessed. Finally, we established a subcutaneous tumor-bearing mouse model to further validate its therapeutic significance in vivo. |
| format | Article |
| id | doaj-art-1a63faaa12974ca4b117c5c06e5b09b5 |
| institution | Kabale University |
| issn | 1471-2407 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj-art-1a63faaa12974ca4b117c5c06e5b09b52025-02-02T12:28:36ZengBMCBMC Cancer1471-24072025-01-0125111810.1186/s12885-025-13567-5NIPAL1 as a prognostic biomarker associated with pancreatic adenocarcinoma progression and immune infiltrationYoulong Zhu0Zongze Qi1Shaoqi Zu2Fangchao Yang3Yanming Wang4Lei Zhu5Xintong Li6Ruixue Li7Hong Zhu8First Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital of Kunming Medical UniversityDepartment of General Surgery, No. 1 Hospital of the Xundian CountyFirst Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital of Kunming Medical UniversityFirst Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital of Kunming Medical UniversityFirst Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital of Kunming Medical UniversityFirst Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital of Kunming Medical UniversityAcademy of Basic Medicine, Kunming Medical UniversityFirst Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital of Kunming Medical UniversityFirst Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital of Kunming Medical UniversityAbstract Pancreatic adenocarcinoma (PAAD) is a highly malignant tumor in the digestive system, with an increasing incidence and mortality rate globally. Recent genetic studies have revealed that the abnormal expression and functional dysregulation of various genes are involved in the occurrence and progression of pancreatic cancer. NIPA-like proteins (NIPAs) are expressed in a variety of cancer types, yet the role of NIPAL1 in cancer remains unclear. Therefore, further research is required to determine its diagnostic significance and understand its biological functions in cancer. Primitive RNA sequencing (RNA-seq) data of PAAD from The Cancer Genome Atlas (TCGA) was utilized for bioinformatics analysis to characterize the expression levels of NIPAL1 in tumor and normal tissues. Differentially expressed genes (DEGs) were identified, and Gene Set Enrichment Analysis (GSEA) was performed to elucidate potential biological mechanisms of NIPAL1 involved in PAAD development. Additionally, we analyzed the correlation between NIPAL1 expression, immune cell infiltration, and PAAD progression. The Genomics of Drug Sensitivity in Cancer (GDSC) database was utilized to investigate the relationship between NIPAL1 expression and the efficacy of common drugs used in chemotherapy and targeted therapy in patients with pancreatic cancer. Subsequently, we predicted five small-molecule drugs targeted at NIPAL1 using molecular docking. Finally, high expression of NIPAL1 in tumor tissues was validated through immunohistochemistry. In pancreatic cancer cell lines, changes in phenotypes such as proliferation, migration, and invasion following the knockdown of NIPAL1 were assessed. Finally, we established a subcutaneous tumor-bearing mouse model to further validate its therapeutic significance in vivo.https://doi.org/10.1186/s12885-025-13567-5Pancreatic cancerNIPAL1PrognosisBiomarkerImmune infiltration |
| spellingShingle | Youlong Zhu Zongze Qi Shaoqi Zu Fangchao Yang Yanming Wang Lei Zhu Xintong Li Ruixue Li Hong Zhu NIPAL1 as a prognostic biomarker associated with pancreatic adenocarcinoma progression and immune infiltration BMC Cancer Pancreatic cancer NIPAL1 Prognosis Biomarker Immune infiltration |
| title | NIPAL1 as a prognostic biomarker associated with pancreatic adenocarcinoma progression and immune infiltration |
| title_full | NIPAL1 as a prognostic biomarker associated with pancreatic adenocarcinoma progression and immune infiltration |
| title_fullStr | NIPAL1 as a prognostic biomarker associated with pancreatic adenocarcinoma progression and immune infiltration |
| title_full_unstemmed | NIPAL1 as a prognostic biomarker associated with pancreatic adenocarcinoma progression and immune infiltration |
| title_short | NIPAL1 as a prognostic biomarker associated with pancreatic adenocarcinoma progression and immune infiltration |
| title_sort | nipal1 as a prognostic biomarker associated with pancreatic adenocarcinoma progression and immune infiltration |
| topic | Pancreatic cancer NIPAL1 Prognosis Biomarker Immune infiltration |
| url | https://doi.org/10.1186/s12885-025-13567-5 |
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