Nitrogen uptake and remobilization from pre- and post-anthesis stages contribute towards grain yield and grain protein concentration in wheat grown in limited nitrogen conditions
Abstract Background In wheat, nitrogen (N) remobilization from vegetative tissues to developing grains largely depends on genetic and environmental factors. The evaluation of genetic potential of crops under limited resource inputs such as limited N supply would provide an opportunity to identify N-...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
CABI
2023-05-01
|
| Series: | CABI Agriculture and Bioscience |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43170-023-00153-7 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832570754528444416 |
|---|---|
| author | Sandeep Sharma Tarun Kumar M. John Foulkes Simon Orford Anju Mahendru Singh Luzie U. Wingen Venkatesh Karnam Lekshmy S. Nair Pranab Kumar Mandal Simon Griffiths Malcolm J. Hawkesford Peter R. Shewry Alison R. Bentley Renu Pandey |
| author_facet | Sandeep Sharma Tarun Kumar M. John Foulkes Simon Orford Anju Mahendru Singh Luzie U. Wingen Venkatesh Karnam Lekshmy S. Nair Pranab Kumar Mandal Simon Griffiths Malcolm J. Hawkesford Peter R. Shewry Alison R. Bentley Renu Pandey |
| author_sort | Sandeep Sharma |
| collection | DOAJ |
| description | Abstract Background In wheat, nitrogen (N) remobilization from vegetative tissues to developing grains largely depends on genetic and environmental factors. The evaluation of genetic potential of crops under limited resource inputs such as limited N supply would provide an opportunity to identify N-efficient lines with improved N utilisation efficiency and yield potential. We assessed the genetic variation in wheat recombinant inbred lines (RILs) for uptake, partitioning, and remobilization of N towards grain, its association with grain protein concentration (GPC) and grain yield. Methods We used the nested association mapping (NAM) population (195 lines) derived by crossing Paragon (P) with CIMMYT core germplasm (P × Cim), Baj (P × Baj), Watkins (P × Wat), and Wyalkatchem (P × Wya). These lines were evaluated in the field for two seasons under limited N supply. The plant sampling was done at anthesis and physiological maturity stages. Various physiological traits were recorded and total N uptake and other N related indices were calculated. The grain protein deviation (GPD) was calculated from the regression of grain yield on GPC. These lines were grouped into different clusters by hierarchical cluster analysis based on grain yield and N-remobilization efficiency (NRE). Results The genetic variation in accumulation of biomass at both pre- and post-anthesis stages were correlated with grain-yield. The NRE significantly correlated with aboveground N uptake at anthesis (AGNa) and grain yield but negatively associated with AGN at post-anthesis (AGNpa) suggesting higher N uptake till anthesis favours high N remobilization during grain filling. Hierarchical cluster analysis of these RILs based on NRE and yield resulted in four clusters, efficient (31), moderately efficient (59), moderately inefficient (58), and inefficient (47). In the N-efficient lines, AGNa contributed to 77% of total N accumulated in grains, while it was 63% in N-inefficient lines. Several N-efficient lines also exhibited positive grain protein deviation (GPD), combining high grain yield and GPC. Among crosses, the P × Cim were superior and N-efficient, while P × Wya responded poorly to low N input. Conclusions We propose that traits favouring pre- or post-anthesis biomass accumulation and pre-anthesis N uptake may be targeted for breeding to improve grain-yield under limited N. The lines with positive GPD, a first report of genotype-dependent GPD associated with both AGNpa and AGNa in wheat, may be used as varieties or genetic resources to improve grain yield with high GPC for sustainable development under limited N conditions. |
| format | Article |
| id | doaj-art-1a4ec08c7ef642b0b4c57685adf170f4 |
| institution | Kabale University |
| issn | 2662-4044 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | CABI |
| record_format | Article |
| series | CABI Agriculture and Bioscience |
| spelling | doaj-art-1a4ec08c7ef642b0b4c57685adf170f42025-02-02T13:57:26ZengCABICABI Agriculture and Bioscience2662-40442023-05-014112010.1186/s43170-023-00153-7Nitrogen uptake and remobilization from pre- and post-anthesis stages contribute towards grain yield and grain protein concentration in wheat grown in limited nitrogen conditionsSandeep Sharma0Tarun Kumar1M. John Foulkes2Simon Orford3Anju Mahendru Singh4Luzie U. Wingen5Venkatesh Karnam6Lekshmy S. Nair7Pranab Kumar Mandal8Simon Griffiths9Malcolm J. Hawkesford10Peter R. Shewry11Alison R. Bentley12Renu Pandey13Division of Plant Physiology, ICAR-Indian Agricultural Research InstituteDivision of Plant Physiology, ICAR-Indian Agricultural Research InstituteDivision of Plant and Crop Sciences, School of Biosciences, University of NottinghamCrop Genetics, John Innes Centre, Norwich Research ParkDivision of Genetics, ICAR-Indian Agricultural Research InstituteCrop Genetics, John Innes Centre, Norwich Research ParkICAR-Indian Institute of Wheat and Barley ResearchDivision of Plant Physiology, ICAR-Indian Agricultural Research InstituteNational Institute of Plant BiotechnologyCrop Genetics, John Innes Centre, Norwich Research ParkPlant Sciences Department, Rothamsted ResearchPlant Sciences Department, Rothamsted ResearchNational Institute for Agricultural BotanyDivision of Plant Physiology, ICAR-Indian Agricultural Research InstituteAbstract Background In wheat, nitrogen (N) remobilization from vegetative tissues to developing grains largely depends on genetic and environmental factors. The evaluation of genetic potential of crops under limited resource inputs such as limited N supply would provide an opportunity to identify N-efficient lines with improved N utilisation efficiency and yield potential. We assessed the genetic variation in wheat recombinant inbred lines (RILs) for uptake, partitioning, and remobilization of N towards grain, its association with grain protein concentration (GPC) and grain yield. Methods We used the nested association mapping (NAM) population (195 lines) derived by crossing Paragon (P) with CIMMYT core germplasm (P × Cim), Baj (P × Baj), Watkins (P × Wat), and Wyalkatchem (P × Wya). These lines were evaluated in the field for two seasons under limited N supply. The plant sampling was done at anthesis and physiological maturity stages. Various physiological traits were recorded and total N uptake and other N related indices were calculated. The grain protein deviation (GPD) was calculated from the regression of grain yield on GPC. These lines were grouped into different clusters by hierarchical cluster analysis based on grain yield and N-remobilization efficiency (NRE). Results The genetic variation in accumulation of biomass at both pre- and post-anthesis stages were correlated with grain-yield. The NRE significantly correlated with aboveground N uptake at anthesis (AGNa) and grain yield but negatively associated with AGN at post-anthesis (AGNpa) suggesting higher N uptake till anthesis favours high N remobilization during grain filling. Hierarchical cluster analysis of these RILs based on NRE and yield resulted in four clusters, efficient (31), moderately efficient (59), moderately inefficient (58), and inefficient (47). In the N-efficient lines, AGNa contributed to 77% of total N accumulated in grains, while it was 63% in N-inefficient lines. Several N-efficient lines also exhibited positive grain protein deviation (GPD), combining high grain yield and GPC. Among crosses, the P × Cim were superior and N-efficient, while P × Wya responded poorly to low N input. Conclusions We propose that traits favouring pre- or post-anthesis biomass accumulation and pre-anthesis N uptake may be targeted for breeding to improve grain-yield under limited N. The lines with positive GPD, a first report of genotype-dependent GPD associated with both AGNpa and AGNa in wheat, may be used as varieties or genetic resources to improve grain yield with high GPC for sustainable development under limited N conditions.https://doi.org/10.1186/s43170-023-00153-7Biomass partitioningGrain protein concentrationGrain protein deviationLow soil nitrogenNitrogen remobilization efficiencyTriticum aestivum |
| spellingShingle | Sandeep Sharma Tarun Kumar M. John Foulkes Simon Orford Anju Mahendru Singh Luzie U. Wingen Venkatesh Karnam Lekshmy S. Nair Pranab Kumar Mandal Simon Griffiths Malcolm J. Hawkesford Peter R. Shewry Alison R. Bentley Renu Pandey Nitrogen uptake and remobilization from pre- and post-anthesis stages contribute towards grain yield and grain protein concentration in wheat grown in limited nitrogen conditions CABI Agriculture and Bioscience Biomass partitioning Grain protein concentration Grain protein deviation Low soil nitrogen Nitrogen remobilization efficiency Triticum aestivum |
| title | Nitrogen uptake and remobilization from pre- and post-anthesis stages contribute towards grain yield and grain protein concentration in wheat grown in limited nitrogen conditions |
| title_full | Nitrogen uptake and remobilization from pre- and post-anthesis stages contribute towards grain yield and grain protein concentration in wheat grown in limited nitrogen conditions |
| title_fullStr | Nitrogen uptake and remobilization from pre- and post-anthesis stages contribute towards grain yield and grain protein concentration in wheat grown in limited nitrogen conditions |
| title_full_unstemmed | Nitrogen uptake and remobilization from pre- and post-anthesis stages contribute towards grain yield and grain protein concentration in wheat grown in limited nitrogen conditions |
| title_short | Nitrogen uptake and remobilization from pre- and post-anthesis stages contribute towards grain yield and grain protein concentration in wheat grown in limited nitrogen conditions |
| title_sort | nitrogen uptake and remobilization from pre and post anthesis stages contribute towards grain yield and grain protein concentration in wheat grown in limited nitrogen conditions |
| topic | Biomass partitioning Grain protein concentration Grain protein deviation Low soil nitrogen Nitrogen remobilization efficiency Triticum aestivum |
| url | https://doi.org/10.1186/s43170-023-00153-7 |
| work_keys_str_mv | AT sandeepsharma nitrogenuptakeandremobilizationfrompreandpostanthesisstagescontributetowardsgrainyieldandgrainproteinconcentrationinwheatgrowninlimitednitrogenconditions AT tarunkumar nitrogenuptakeandremobilizationfrompreandpostanthesisstagescontributetowardsgrainyieldandgrainproteinconcentrationinwheatgrowninlimitednitrogenconditions AT mjohnfoulkes nitrogenuptakeandremobilizationfrompreandpostanthesisstagescontributetowardsgrainyieldandgrainproteinconcentrationinwheatgrowninlimitednitrogenconditions AT simonorford nitrogenuptakeandremobilizationfrompreandpostanthesisstagescontributetowardsgrainyieldandgrainproteinconcentrationinwheatgrowninlimitednitrogenconditions AT anjumahendrusingh nitrogenuptakeandremobilizationfrompreandpostanthesisstagescontributetowardsgrainyieldandgrainproteinconcentrationinwheatgrowninlimitednitrogenconditions AT luzieuwingen nitrogenuptakeandremobilizationfrompreandpostanthesisstagescontributetowardsgrainyieldandgrainproteinconcentrationinwheatgrowninlimitednitrogenconditions AT venkateshkarnam nitrogenuptakeandremobilizationfrompreandpostanthesisstagescontributetowardsgrainyieldandgrainproteinconcentrationinwheatgrowninlimitednitrogenconditions AT lekshmysnair nitrogenuptakeandremobilizationfrompreandpostanthesisstagescontributetowardsgrainyieldandgrainproteinconcentrationinwheatgrowninlimitednitrogenconditions AT pranabkumarmandal nitrogenuptakeandremobilizationfrompreandpostanthesisstagescontributetowardsgrainyieldandgrainproteinconcentrationinwheatgrowninlimitednitrogenconditions AT simongriffiths nitrogenuptakeandremobilizationfrompreandpostanthesisstagescontributetowardsgrainyieldandgrainproteinconcentrationinwheatgrowninlimitednitrogenconditions AT malcolmjhawkesford nitrogenuptakeandremobilizationfrompreandpostanthesisstagescontributetowardsgrainyieldandgrainproteinconcentrationinwheatgrowninlimitednitrogenconditions AT peterrshewry nitrogenuptakeandremobilizationfrompreandpostanthesisstagescontributetowardsgrainyieldandgrainproteinconcentrationinwheatgrowninlimitednitrogenconditions AT alisonrbentley nitrogenuptakeandremobilizationfrompreandpostanthesisstagescontributetowardsgrainyieldandgrainproteinconcentrationinwheatgrowninlimitednitrogenconditions AT renupandey nitrogenuptakeandremobilizationfrompreandpostanthesisstagescontributetowardsgrainyieldandgrainproteinconcentrationinwheatgrowninlimitednitrogenconditions |