Impact of IL28B genetic variant's and viral genotype on treatment outcome of hepatitis C infected patients

Introduction: Viral genotype and variation in host genes involved in the immune response may predict the treatment response in patients infected with HCV. The present study was designed to determine the distribution pattern of HCV and host genotypes in Chronic Hepatitis C (CHC) patients and their a...

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Main Authors: Mosin S Khan, Abid Shoukat, Syed Mudassar, Zaffar Kawoosa, Altaf H Shah, Showkat Ali Zargar
Format: Article
Language:English
Published: The Journal of Infection in Developing Countries 2018-09-01
Series:Journal of Infection in Developing Countries
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Online Access:https://jidc.org/index.php/journal/article/view/10175
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author Mosin S Khan
Abid Shoukat
Syed Mudassar
Zaffar Kawoosa
Altaf H Shah
Showkat Ali Zargar
author_facet Mosin S Khan
Abid Shoukat
Syed Mudassar
Zaffar Kawoosa
Altaf H Shah
Showkat Ali Zargar
author_sort Mosin S Khan
collection DOAJ
description Introduction: Viral genotype and variation in host genes involved in the immune response may predict the treatment response in patients infected with HCV. The present study was designed to determine the distribution pattern of HCV and host genotypes in Chronic Hepatitis C (CHC) patients and their association with virological response and other risk factors. Methodology: Two hundred and fifty (n = 250) HCV positive patients were included in the study. HCV and Interleukin 28B (IL28B) genotyping was carried out by PCR-RFLP. Results: Viral genotype 3 was the predominant genotype seen in 187 (74.8%) patients. Wild genotype predominated in rs12979860, rs12980275 and rs8099917 SNP of IL28B gene. A significant difference was found in end stage virological response (EVR) between HCV genotype 1 infected patients with wild and variant genotype for rs12980275 and rs8099917 SNPs respectively (P < 0.05). On multivariate analysis all the SNPs were found to be associated with each other (P < 0.05) with rs12980275 SNP associated with history of Jaundice (P < 0.05). Viral genotype 3 was significantly associated with age (< 50 years) and rapid virological response (RVR) while as viral genotype 1 was significantly associated with history of surgery on multivariate analysis (P < 0.05). Conclusions: The viral genotype and IL28B polymorphisms are important factors to personalize antiviral therapy of patients with CHC.
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spelling doaj-art-1a49fb9985e14c0aaa85b4e0f674300a2025-08-20T03:48:46ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802018-09-01120910.3855/jidc.10175Impact of IL28B genetic variant's and viral genotype on treatment outcome of hepatitis C infected patientsMosin S Khan0Abid Shoukat1Syed Mudassar2Zaffar Kawoosa3Altaf H Shah4Showkat Ali Zargar5Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, IndiaSher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, IndiaSher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, IndiaSher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, IndiaSher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, IndiaSher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, India Introduction: Viral genotype and variation in host genes involved in the immune response may predict the treatment response in patients infected with HCV. The present study was designed to determine the distribution pattern of HCV and host genotypes in Chronic Hepatitis C (CHC) patients and their association with virological response and other risk factors. Methodology: Two hundred and fifty (n = 250) HCV positive patients were included in the study. HCV and Interleukin 28B (IL28B) genotyping was carried out by PCR-RFLP. Results: Viral genotype 3 was the predominant genotype seen in 187 (74.8%) patients. Wild genotype predominated in rs12979860, rs12980275 and rs8099917 SNP of IL28B gene. A significant difference was found in end stage virological response (EVR) between HCV genotype 1 infected patients with wild and variant genotype for rs12980275 and rs8099917 SNPs respectively (P < 0.05). On multivariate analysis all the SNPs were found to be associated with each other (P < 0.05) with rs12980275 SNP associated with history of Jaundice (P < 0.05). Viral genotype 3 was significantly associated with age (< 50 years) and rapid virological response (RVR) while as viral genotype 1 was significantly associated with history of surgery on multivariate analysis (P < 0.05). Conclusions: The viral genotype and IL28B polymorphisms are important factors to personalize antiviral therapy of patients with CHC. https://jidc.org/index.php/journal/article/view/10175Hepatitis Cchronic hepatitis CIL28Bviral genotypePCR-RFLPhepatocellular carcinoma
spellingShingle Mosin S Khan
Abid Shoukat
Syed Mudassar
Zaffar Kawoosa
Altaf H Shah
Showkat Ali Zargar
Impact of IL28B genetic variant's and viral genotype on treatment outcome of hepatitis C infected patients
Journal of Infection in Developing Countries
Hepatitis C
chronic hepatitis C
IL28B
viral genotype
PCR-RFLP
hepatocellular carcinoma
title Impact of IL28B genetic variant's and viral genotype on treatment outcome of hepatitis C infected patients
title_full Impact of IL28B genetic variant's and viral genotype on treatment outcome of hepatitis C infected patients
title_fullStr Impact of IL28B genetic variant's and viral genotype on treatment outcome of hepatitis C infected patients
title_full_unstemmed Impact of IL28B genetic variant's and viral genotype on treatment outcome of hepatitis C infected patients
title_short Impact of IL28B genetic variant's and viral genotype on treatment outcome of hepatitis C infected patients
title_sort impact of il28b genetic variant s and viral genotype on treatment outcome of hepatitis c infected patients
topic Hepatitis C
chronic hepatitis C
IL28B
viral genotype
PCR-RFLP
hepatocellular carcinoma
url https://jidc.org/index.php/journal/article/view/10175
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