Utility of Cand PCR in the Diagnosis of Vulvovaginal Candidiasis in Pregnant Women
Vulvovaginal candidiasis (VVC) can lead to multiple complications when it occurs during pregnancy, so it is necessary to diagnose it promptly for effective treatment. Traditional methods for identifying <i>Candida</i> spp. are often too time-consuming and have limited specificity and sen...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/11/1/5 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832588181557477376 |
|---|---|
| author | Eduardo García-Salazar Paola Betancourt-Cisneros Xóchitl Ramírez-Magaña Hugo Díaz-Huerta Erick Martínez-Herrera María Guadalupe Frías-De-León |
| author_facet | Eduardo García-Salazar Paola Betancourt-Cisneros Xóchitl Ramírez-Magaña Hugo Díaz-Huerta Erick Martínez-Herrera María Guadalupe Frías-De-León |
| author_sort | Eduardo García-Salazar |
| collection | DOAJ |
| description | Vulvovaginal candidiasis (VVC) can lead to multiple complications when it occurs during pregnancy, so it is necessary to diagnose it promptly for effective treatment. Traditional methods for identifying <i>Candida</i> spp. are often too time-consuming and have limited specificity and sensitivity. In this work, we evaluated the diagnostic utility of an endpoint PCR assay (Cand PCR) in vaginal swab specimens. Using a cotton swab, 108 vaginal swab samples were taken from pregnant women who consented to participate in the study. The samples were inoculated in Sabouraud agar plates (the gold standard) and subsequently used to extract DNA directly from the exudate. The yeasts isolated from the Sabouraud agar were identified in CHROMagar™ Candida. DNA extracted from vaginal swabs was amplified by Cand PCR. Based on the results of the Cand PCR and the gold standard, sensitivity (S), specificity (E), positive predictive values (PPVs), and negative predictive values (NPVs) were determined. Cand PCR presented an S = 65%, E = 100%, PPV = 100% and NPV = 91%. Cand PCR showed low sensitivity for detecting <i>Candida</i> spp. directly from vaginal swabs, but it was useful for identifying the etiologic agent and reducing the time to obtain the result, which is usually at least 48 h. |
| format | Article |
| id | doaj-art-1a3b4e80aad54539983c30e31b781cba |
| institution | Kabale University |
| issn | 2309-608X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-1a3b4e80aad54539983c30e31b781cba2025-01-24T13:37:11ZengMDPI AGJournal of Fungi2309-608X2024-12-01111510.3390/jof11010005Utility of Cand PCR in the Diagnosis of Vulvovaginal Candidiasis in Pregnant WomenEduardo García-Salazar0Paola Betancourt-Cisneros1Xóchitl Ramírez-Magaña2Hugo Díaz-Huerta3Erick Martínez-Herrera4María Guadalupe Frías-De-León5Laboratorio de Micología Molecular, Unidad de Investigación Biomédica, Hospital Regional de Alta Especialidad de Ixtapaluca, Instituto Mexicano de Seguro Social para el Bienestar (IMSS-BIENESTAR), Carretera Federal México-Puebla Km 34.5, Ixtapaluca CP 56530, MexicoLaboratorio de Micología Molecular, Unidad de Investigación Biomédica, Hospital Regional de Alta Especialidad de Ixtapaluca, Instituto Mexicano de Seguro Social para el Bienestar (IMSS-BIENESTAR), Carretera Federal México-Puebla Km 34.5, Ixtapaluca CP 56530, MexicoServicio de Ginecología y Obstetricia, Hospital Regional de Alta Especialidad de Ixtapaluca, Instituto Mexicano de Seguro Social para el Bienestar (IMSS-BIENESTAR), Carretera Federal México-Puebla Km 34.5, Ixtapaluca CP 56530, MexicoUnidad de Calidad y Riesgo Biológico, Hospital Regional de Alta Especialidad de Ixtapaluca, Instituto Mexicano de Seguro Social para el Bienestar (IMSS-BIENESTAR), Carretera Federal México-Puebla Km 34.5, Ixtapaluca CP 56530, MexicoSección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s/n, Col. Casco de Santo Tomas, Alcaldía Miguel Hidalgo, México City CP 11340, MexicoLaboratorio de Micología Molecular, Unidad de Investigación Biomédica, Hospital Regional de Alta Especialidad de Ixtapaluca, Instituto Mexicano de Seguro Social para el Bienestar (IMSS-BIENESTAR), Carretera Federal México-Puebla Km 34.5, Ixtapaluca CP 56530, MexicoVulvovaginal candidiasis (VVC) can lead to multiple complications when it occurs during pregnancy, so it is necessary to diagnose it promptly for effective treatment. Traditional methods for identifying <i>Candida</i> spp. are often too time-consuming and have limited specificity and sensitivity. In this work, we evaluated the diagnostic utility of an endpoint PCR assay (Cand PCR) in vaginal swab specimens. Using a cotton swab, 108 vaginal swab samples were taken from pregnant women who consented to participate in the study. The samples were inoculated in Sabouraud agar plates (the gold standard) and subsequently used to extract DNA directly from the exudate. The yeasts isolated from the Sabouraud agar were identified in CHROMagar™ Candida. DNA extracted from vaginal swabs was amplified by Cand PCR. Based on the results of the Cand PCR and the gold standard, sensitivity (S), specificity (E), positive predictive values (PPVs), and negative predictive values (NPVs) were determined. Cand PCR presented an S = 65%, E = 100%, PPV = 100% and NPV = 91%. Cand PCR showed low sensitivity for detecting <i>Candida</i> spp. directly from vaginal swabs, but it was useful for identifying the etiologic agent and reducing the time to obtain the result, which is usually at least 48 h.https://www.mdpi.com/2309-608X/11/1/5<i>Candida</i> spp.mycotic vulvovaginitismolecular identificationdiagnosispregnancy |
| spellingShingle | Eduardo García-Salazar Paola Betancourt-Cisneros Xóchitl Ramírez-Magaña Hugo Díaz-Huerta Erick Martínez-Herrera María Guadalupe Frías-De-León Utility of Cand PCR in the Diagnosis of Vulvovaginal Candidiasis in Pregnant Women Journal of Fungi <i>Candida</i> spp. mycotic vulvovaginitis molecular identification diagnosis pregnancy |
| title | Utility of Cand PCR in the Diagnosis of Vulvovaginal Candidiasis in Pregnant Women |
| title_full | Utility of Cand PCR in the Diagnosis of Vulvovaginal Candidiasis in Pregnant Women |
| title_fullStr | Utility of Cand PCR in the Diagnosis of Vulvovaginal Candidiasis in Pregnant Women |
| title_full_unstemmed | Utility of Cand PCR in the Diagnosis of Vulvovaginal Candidiasis in Pregnant Women |
| title_short | Utility of Cand PCR in the Diagnosis of Vulvovaginal Candidiasis in Pregnant Women |
| title_sort | utility of cand pcr in the diagnosis of vulvovaginal candidiasis in pregnant women |
| topic | <i>Candida</i> spp. mycotic vulvovaginitis molecular identification diagnosis pregnancy |
| url | https://www.mdpi.com/2309-608X/11/1/5 |
| work_keys_str_mv | AT eduardogarciasalazar utilityofcandpcrinthediagnosisofvulvovaginalcandidiasisinpregnantwomen AT paolabetancourtcisneros utilityofcandpcrinthediagnosisofvulvovaginalcandidiasisinpregnantwomen AT xochitlramirezmagana utilityofcandpcrinthediagnosisofvulvovaginalcandidiasisinpregnantwomen AT hugodiazhuerta utilityofcandpcrinthediagnosisofvulvovaginalcandidiasisinpregnantwomen AT erickmartinezherrera utilityofcandpcrinthediagnosisofvulvovaginalcandidiasisinpregnantwomen AT mariaguadalupefriasdeleon utilityofcandpcrinthediagnosisofvulvovaginalcandidiasisinpregnantwomen |