Alsterpaullone, a Cyclin-Dependent Kinase Inhibitor, Mediated Toxicity in HeLa Cells through Apoptosis-Inducing Effect

Alsterpaullone, a small molecule cyclin-dependent kinase (CDK) inhibitor, regulates the cell cycle progression. Beyond death-inducing properties, we identified the effect of alsterpaullone on cycle procedure and apoptosis of HeLa cell. It was found that alsterpaullone inhibited HeLa cells in a time-...

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Main Authors: Chunying Cui, Yuji Wang, Yaonan Wang, Ming Zhao, Shiqi Peng
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:Journal of Analytical Methods in Chemistry
Online Access:http://dx.doi.org/10.1155/2013/602091
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author Chunying Cui
Yuji Wang
Yaonan Wang
Ming Zhao
Shiqi Peng
author_facet Chunying Cui
Yuji Wang
Yaonan Wang
Ming Zhao
Shiqi Peng
author_sort Chunying Cui
collection DOAJ
description Alsterpaullone, a small molecule cyclin-dependent kinase (CDK) inhibitor, regulates the cell cycle progression. Beyond death-inducing properties, we identified the effect of alsterpaullone on cycle procedure and apoptosis of HeLa cell. It was found that alsterpaullone inhibited HeLa cells in a time-dependent (0–72 h) and dose-dependent (0–30 μM) manner. In the presence of alsterpaullone, HeLa cells were arrested in G2/M prior to undergoing apoptosis via a mechanism that is involved in the regulation of various antiapoptotic genes, DNA-repair, transcription, and cell cycle progression. Compared to controls, alsterpaullone effectively prevented HeLa cells from entering S-phase. These potential therapeutic efficacies could be correlated with the activation of caspase-3.
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institution Kabale University
issn 2090-8865
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language English
publishDate 2013-01-01
publisher Wiley
record_format Article
series Journal of Analytical Methods in Chemistry
spelling doaj-art-1a3537e5169d43ecbc812850d92c5b502025-02-03T06:10:49ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/602091602091Alsterpaullone, a Cyclin-Dependent Kinase Inhibitor, Mediated Toxicity in HeLa Cells through Apoptosis-Inducing EffectChunying Cui0Yuji Wang1Yaonan Wang2Ming Zhao3Shiqi Peng4College of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, ChinaCollege of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, ChinaMedical Experiment and Test Center, Capital Medical University, Beijing 100069, ChinaCollege of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, ChinaCollege of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, ChinaAlsterpaullone, a small molecule cyclin-dependent kinase (CDK) inhibitor, regulates the cell cycle progression. Beyond death-inducing properties, we identified the effect of alsterpaullone on cycle procedure and apoptosis of HeLa cell. It was found that alsterpaullone inhibited HeLa cells in a time-dependent (0–72 h) and dose-dependent (0–30 μM) manner. In the presence of alsterpaullone, HeLa cells were arrested in G2/M prior to undergoing apoptosis via a mechanism that is involved in the regulation of various antiapoptotic genes, DNA-repair, transcription, and cell cycle progression. Compared to controls, alsterpaullone effectively prevented HeLa cells from entering S-phase. These potential therapeutic efficacies could be correlated with the activation of caspase-3.http://dx.doi.org/10.1155/2013/602091
spellingShingle Chunying Cui
Yuji Wang
Yaonan Wang
Ming Zhao
Shiqi Peng
Alsterpaullone, a Cyclin-Dependent Kinase Inhibitor, Mediated Toxicity in HeLa Cells through Apoptosis-Inducing Effect
Journal of Analytical Methods in Chemistry
title Alsterpaullone, a Cyclin-Dependent Kinase Inhibitor, Mediated Toxicity in HeLa Cells through Apoptosis-Inducing Effect
title_full Alsterpaullone, a Cyclin-Dependent Kinase Inhibitor, Mediated Toxicity in HeLa Cells through Apoptosis-Inducing Effect
title_fullStr Alsterpaullone, a Cyclin-Dependent Kinase Inhibitor, Mediated Toxicity in HeLa Cells through Apoptosis-Inducing Effect
title_full_unstemmed Alsterpaullone, a Cyclin-Dependent Kinase Inhibitor, Mediated Toxicity in HeLa Cells through Apoptosis-Inducing Effect
title_short Alsterpaullone, a Cyclin-Dependent Kinase Inhibitor, Mediated Toxicity in HeLa Cells through Apoptosis-Inducing Effect
title_sort alsterpaullone a cyclin dependent kinase inhibitor mediated toxicity in hela cells through apoptosis inducing effect
url http://dx.doi.org/10.1155/2013/602091
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AT yaonanwang alsterpaulloneacyclindependentkinaseinhibitormediatedtoxicityinhelacellsthroughapoptosisinducingeffect
AT mingzhao alsterpaulloneacyclindependentkinaseinhibitormediatedtoxicityinhelacellsthroughapoptosisinducingeffect
AT shiqipeng alsterpaulloneacyclindependentkinaseinhibitormediatedtoxicityinhelacellsthroughapoptosisinducingeffect