Alsterpaullone, a Cyclin-Dependent Kinase Inhibitor, Mediated Toxicity in HeLa Cells through Apoptosis-Inducing Effect
Alsterpaullone, a small molecule cyclin-dependent kinase (CDK) inhibitor, regulates the cell cycle progression. Beyond death-inducing properties, we identified the effect of alsterpaullone on cycle procedure and apoptosis of HeLa cell. It was found that alsterpaullone inhibited HeLa cells in a time-...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/602091 |
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| author | Chunying Cui Yuji Wang Yaonan Wang Ming Zhao Shiqi Peng |
| author_facet | Chunying Cui Yuji Wang Yaonan Wang Ming Zhao Shiqi Peng |
| author_sort | Chunying Cui |
| collection | DOAJ |
| description | Alsterpaullone, a small molecule cyclin-dependent kinase (CDK) inhibitor, regulates the cell cycle progression. Beyond death-inducing properties, we identified the effect of alsterpaullone on cycle procedure and apoptosis of HeLa cell. It was found that alsterpaullone inhibited HeLa cells in a time-dependent (0–72 h) and dose-dependent (0–30 μM) manner. In the presence of alsterpaullone, HeLa cells were arrested in G2/M prior to undergoing apoptosis via a mechanism that is involved in the regulation of various antiapoptotic genes, DNA-repair, transcription, and cell cycle progression. Compared to controls, alsterpaullone effectively prevented HeLa cells from entering S-phase. These potential therapeutic efficacies could be correlated with the activation of caspase-3. |
| format | Article |
| id | doaj-art-1a3537e5169d43ecbc812850d92c5b50 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-1a3537e5169d43ecbc812850d92c5b502025-02-03T06:10:49ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/602091602091Alsterpaullone, a Cyclin-Dependent Kinase Inhibitor, Mediated Toxicity in HeLa Cells through Apoptosis-Inducing EffectChunying Cui0Yuji Wang1Yaonan Wang2Ming Zhao3Shiqi Peng4College of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, ChinaCollege of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, ChinaMedical Experiment and Test Center, Capital Medical University, Beijing 100069, ChinaCollege of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, ChinaCollege of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, ChinaAlsterpaullone, a small molecule cyclin-dependent kinase (CDK) inhibitor, regulates the cell cycle progression. Beyond death-inducing properties, we identified the effect of alsterpaullone on cycle procedure and apoptosis of HeLa cell. It was found that alsterpaullone inhibited HeLa cells in a time-dependent (0–72 h) and dose-dependent (0–30 μM) manner. In the presence of alsterpaullone, HeLa cells were arrested in G2/M prior to undergoing apoptosis via a mechanism that is involved in the regulation of various antiapoptotic genes, DNA-repair, transcription, and cell cycle progression. Compared to controls, alsterpaullone effectively prevented HeLa cells from entering S-phase. These potential therapeutic efficacies could be correlated with the activation of caspase-3.http://dx.doi.org/10.1155/2013/602091 |
| spellingShingle | Chunying Cui Yuji Wang Yaonan Wang Ming Zhao Shiqi Peng Alsterpaullone, a Cyclin-Dependent Kinase Inhibitor, Mediated Toxicity in HeLa Cells through Apoptosis-Inducing Effect Journal of Analytical Methods in Chemistry |
| title | Alsterpaullone, a Cyclin-Dependent Kinase Inhibitor, Mediated Toxicity in HeLa Cells through Apoptosis-Inducing Effect |
| title_full | Alsterpaullone, a Cyclin-Dependent Kinase Inhibitor, Mediated Toxicity in HeLa Cells through Apoptosis-Inducing Effect |
| title_fullStr | Alsterpaullone, a Cyclin-Dependent Kinase Inhibitor, Mediated Toxicity in HeLa Cells through Apoptosis-Inducing Effect |
| title_full_unstemmed | Alsterpaullone, a Cyclin-Dependent Kinase Inhibitor, Mediated Toxicity in HeLa Cells through Apoptosis-Inducing Effect |
| title_short | Alsterpaullone, a Cyclin-Dependent Kinase Inhibitor, Mediated Toxicity in HeLa Cells through Apoptosis-Inducing Effect |
| title_sort | alsterpaullone a cyclin dependent kinase inhibitor mediated toxicity in hela cells through apoptosis inducing effect |
| url | http://dx.doi.org/10.1155/2013/602091 |
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