Study of the role of transmembrane emp24 domain-containing protein 2 in oral squamous cell carcinoma
Abstract Objective This study aimed to investigate the role of transmembrane emp24 domain-containing protein 2 (TMED2) in oral squamous cell carcinoma (OSCC). Methodology A bioinformatics analysis was first conducted to explore TMED2 expression in OSCC and its relation with overall survival. The...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of São Paulo
2025-01-01
|
| Series: | Journal of Applied Oral Science |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572025000100404&lng=en&tlng=en |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832542219522801664 |
|---|---|
| author | RUAN Zhao-wei JIAO Xue-feng XIAO Gao-tian CHEN Jin-ling LI Jun LV Shu-ying |
| author_facet | RUAN Zhao-wei JIAO Xue-feng XIAO Gao-tian CHEN Jin-ling LI Jun LV Shu-ying |
| author_sort | RUAN Zhao-wei |
| collection | DOAJ |
| description | Abstract Objective This study aimed to investigate the role of transmembrane emp24 domain-containing protein 2 (TMED2) in oral squamous cell carcinoma (OSCC). Methodology A bioinformatics analysis was first conducted to explore TMED2 expression in OSCC and its relation with overall survival. The analysis results were further verified by assessing TMED2 expression levels in human normal oral keratinocyte cells and human OSCC cell lines using quantitative real-time polymerase chain reaction and the Western blot. Finally, the effects of TMED2 knockdown and overexpression on the expression levels of TMED2, ADP-ribosylation factor 1, extracellular signal-regulated kinase ½, and phospho-extracellular signal-regulated kinase ½ proteins were examined in cells using the Western blot. Results The GEPIA2 database showed that OSCC tissues expressed more TMED2 than normal tissues. At the cellular level, TMED2 expression significantly increased in SCC-4, HSC-3, and CAL-27 cells than in human normal oral keratinocyte cells. TMED2 knockdown reduced cell proliferation, increased the apoptosis rate in SCC-4 cells, and led to a higher proportion of cells in the G0/G1 phase and a lower proportion in the S phase. Conclusion TMED2 may promote OSCC cell proliferation and inhibit apoptosis, potentially by activation of the ADP-ribosylation factor 1/ extracellular signal-regulated kinase ½ signaling pathway. |
| format | Article |
| id | doaj-art-1a2b4f9ce41a459b8be2c55649a477bb |
| institution | Kabale University |
| issn | 1678-7765 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | University of São Paulo |
| record_format | Article |
| series | Journal of Applied Oral Science |
| spelling | doaj-art-1a2b4f9ce41a459b8be2c55649a477bb2025-02-04T07:38:02ZengUniversity of São PauloJournal of Applied Oral Science1678-77652025-01-013310.1590/1678-7757-2024-0305Study of the role of transmembrane emp24 domain-containing protein 2 in oral squamous cell carcinomaRUAN Zhao-weiJIAO Xue-fengXIAO Gao-tianCHEN Jin-lingLI JunLV Shu-yinghttps://orcid.org/0009-0005-1306-7436Abstract Objective This study aimed to investigate the role of transmembrane emp24 domain-containing protein 2 (TMED2) in oral squamous cell carcinoma (OSCC). Methodology A bioinformatics analysis was first conducted to explore TMED2 expression in OSCC and its relation with overall survival. The analysis results were further verified by assessing TMED2 expression levels in human normal oral keratinocyte cells and human OSCC cell lines using quantitative real-time polymerase chain reaction and the Western blot. Finally, the effects of TMED2 knockdown and overexpression on the expression levels of TMED2, ADP-ribosylation factor 1, extracellular signal-regulated kinase ½, and phospho-extracellular signal-regulated kinase ½ proteins were examined in cells using the Western blot. Results The GEPIA2 database showed that OSCC tissues expressed more TMED2 than normal tissues. At the cellular level, TMED2 expression significantly increased in SCC-4, HSC-3, and CAL-27 cells than in human normal oral keratinocyte cells. TMED2 knockdown reduced cell proliferation, increased the apoptosis rate in SCC-4 cells, and led to a higher proportion of cells in the G0/G1 phase and a lower proportion in the S phase. Conclusion TMED2 may promote OSCC cell proliferation and inhibit apoptosis, potentially by activation of the ADP-ribosylation factor 1/ extracellular signal-regulated kinase ½ signaling pathway.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572025000100404&lng=en&tlng=enOral squamous cell carcinomaTMED2ARF1ERK |
| spellingShingle | RUAN Zhao-wei JIAO Xue-feng XIAO Gao-tian CHEN Jin-ling LI Jun LV Shu-ying Study of the role of transmembrane emp24 domain-containing protein 2 in oral squamous cell carcinoma Journal of Applied Oral Science Oral squamous cell carcinoma TMED2 ARF1 ERK |
| title | Study of the role of transmembrane emp24 domain-containing protein 2 in oral squamous cell carcinoma |
| title_full | Study of the role of transmembrane emp24 domain-containing protein 2 in oral squamous cell carcinoma |
| title_fullStr | Study of the role of transmembrane emp24 domain-containing protein 2 in oral squamous cell carcinoma |
| title_full_unstemmed | Study of the role of transmembrane emp24 domain-containing protein 2 in oral squamous cell carcinoma |
| title_short | Study of the role of transmembrane emp24 domain-containing protein 2 in oral squamous cell carcinoma |
| title_sort | study of the role of transmembrane emp24 domain containing protein 2 in oral squamous cell carcinoma |
| topic | Oral squamous cell carcinoma TMED2 ARF1 ERK |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572025000100404&lng=en&tlng=en |
| work_keys_str_mv | AT ruanzhaowei studyoftheroleoftransmembraneemp24domaincontainingprotein2inoralsquamouscellcarcinoma AT jiaoxuefeng studyoftheroleoftransmembraneemp24domaincontainingprotein2inoralsquamouscellcarcinoma AT xiaogaotian studyoftheroleoftransmembraneemp24domaincontainingprotein2inoralsquamouscellcarcinoma AT chenjinling studyoftheroleoftransmembraneemp24domaincontainingprotein2inoralsquamouscellcarcinoma AT lijun studyoftheroleoftransmembraneemp24domaincontainingprotein2inoralsquamouscellcarcinoma AT lvshuying studyoftheroleoftransmembraneemp24domaincontainingprotein2inoralsquamouscellcarcinoma |